Spatial dispersal of porcine reproductive and respiratory syndrome virus-contaminated flies after contact with experimentally infected pigs.

Objective: To determine whether flies can acquire porcine reproductive and respiratory syndrome virus (PRRSV) and disperse the virus throughout a designated area.

Animals: 60 four-month-old pigs.

Procedure: On day 0, 28 of 60 pigs were inoculated with PRRSV MN 30-100 (index variant).

Detection of bovine viral diarrhea virus by TaqMan reverse transcription polymerase chain reaction.

Detection and elimination of calves and cows persistently infected with bovine viral diarrhea virus (BVDV) is important for the control of this pathogen. Historically, BVDV detection involved cell culture isolation followed by virus detection through immunofluorescence or immunoperoxidase monolayer assay (IPMA) methods. More recently, immunohistochemistry (IHC) has been added as a routine test for BVDV detection.