Death and mourning in times of COVID-19. The experience of Zimbabwean migrants in South Africa.

The paper deals with the effects of the regulations and restrictions on the handling of corpses and funerals among Zimbabwean migrant families who lost relatives in South Africa during the COVID-19 epidemic in the years 2020 and 2021. Interviews were conducted with members of this migrant community. The interviews revealed a range of affective and material dimensions entangled in these multiple losses, highlighting therefore experiences of truncated grief.

Ability of new protected areas to counteract losses from downgrading, downsizing, and degazettement.

Protected area downgrading, downsizing, and degazettement (PADDD) is a common occurrence. Although PADDD is expected to weaken biodiversity protection, PADDD offsets and new unrelated protected areas (PAs) could help restore representation of biodiversity features to the reserve network affected by PADDD. Globally, we analyzed 16 territories with terrestrial PADDD and 4 territories with marine PADDD from 2011 to 2020.

Gasdermin D deficiency attenuates development of ascending aortic dissections in a novel mouse model.

Background: Thoracic aortic dissection (TAD) is a silent killer. Approximately two-thirds of the cases occur in the ascending aorta (i.e.

Proteomic analysis of granulomas from cattle and pigs naturally infected with complex by MALDI imaging.

Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has recently gained prominence for its ability to provide molecular and spatial information in tissue sections. This technology has the potential to uncover novel insights into proteins and other molecules in biological and immunological pathways activated along diseases with a complex host-pathogen interaction, such as animal tuberculosis. Thus, the present study conducted a data analysis of protein signature in granulomas of cattle and pigs naturally infected with the complex (MTC), identifying biological and immunological signaling pathways activated throughout the disease.

Applying deep learning on social media to investigate cultural ecosystem services in protected areas worldwide.

Protected areas (PAs) are the cornerstone of conservation efforts. Although they provide many benefits to humanity, the variability in the provision of cultural ecosystem services (CES) among global PAs remains unknown. To investigate this, we combined Convolutional Neural Networks with hierarchical clustering to categorize photos from Flickr taken in PAs worldwide.

The scene of lung pathology during PRRSV-1 infection.

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically important infectious diseases for the pig industry worldwide. The disease was firstly reported in 1987 and became endemic in many countries. Since then, outbreaks caused by strains of high virulence have been reported several times in Asia, America and Europe.

Using satellite data to assess spatial drivers of bird diversity.

Birds are useful indicators of overall biodiversity, which continues to decline globally, despite targets to reduce its loss. The aim of this paper is to understand the importance of different spatial drivers for modelling bird distributions. Specifically, it assesses the importance of satellite-derived measures of habitat productivity, heterogeneity and landscape structure for modelling bird diversity across Great Britain.

Intranational synergies and trade-offs reveal common and differentiated priorities of sustainable development goals in China.

Accelerating efforts for the Sustainable Development Goals requires understanding their synergies and trade-offs at the national and sub-national levels, which will help identify the key hurdles and opportunities to prioritize them in an indivisible manner for a country. Here, we present the importance of the 17 goals through synergy and trade-off networks. Our results reveal that 19 provinces show the highest trade-offs in SDG13 (Combating Climate Change) or SDG5 (Gender Equality) consistent with the national level, with other 12 provinces varying.

Droplet digital PCR as alternative to microbiological culture for complex detection in bovine lymph node tissue samples.

Introduction: Bovine tuberculosis (bTB) caused by complex (MTC) remains a significant concern for public health. Direct real-time PCR and droplet digital PCR (ddPCR) are proposed as alternative tools to enhance diagnostic precision and efficiency. This study aims to assess the diagnostic performance of a ddPCR assay targeting IS for the detection of MTC DNA in both microbiological culture and fresh lymph node (LN) tissue samples obtained from cattle, in comparison with the established reference standard, the microbiological culture followed by real-time PCR.

Climate change market-driven poleward shifts in cropland production create opportunities for tropical biodiversity conservation and habitat restoration.

Although the impacts of climate change on the yields of crops have been studied, how these changes will result in the eventual realized crop production through market feedbacks has received little attention. Using a combination of attainable yield predictions for wheat, rice, maize, soybean and sugarcane, computable general equilibrium and land rent models, we project market impacts and crop-specific land-use change up to 2100 and the resulting implications for carbon and biodiversity. The results show a general increase in crop prices in tropical regions and a decrease in sub-tropical and temperate regions.

Macrophage polarization in lymph node granulomas from cattle and pigs naturally infected with complex.

Tuberculosis in animals is caused by members of the complex (MTC), with the tuberculous granuloma being the main characteristic lesion. The macrophage is the main cell type involved in the development of the granuloma and presents a wide plasticity ranging from polarization to classically activated or pro-inflammatory macrophages (M1) or to alternatively activated or anti-inflammatory macrophages (M2). Thus, this study aimed to analyze macrophage polarization in granulomas from cattle and pig lymph nodes naturally infected with MTC.

Biodiversity footprints of 151 popular dishes from around the world.

Habitat loss for food production is a key threat to global biodiversity. Despite the importance of dietary choices on our capacity to mitigate the on-going biodiversity crisis, unlike with specific ingredients or products, consumers have limited information on the biodiversity implications of choosing to eat a certain popular dish. Here we estimated the biodiversity footprints of 151 popular local dishes from around the world when globally and locally produced and after calorical content standardization.

Time to Recurrence of Ameloblastoma and Associated Factors in a Multi-institutional Black Patient Cohort.

Ameloblastoma is a highly recurrent odontogenic neoplasm with variable global distribution. However, impact of race and ethnicity on ameloblastoma recurrence are still unclear. The primary aim of this study was to assess duration of time between primary and recurrent ameloblastomas in a predominantly Black multi-institutional patient cohort and secondarily to determine whether recurrent ameloblastomas are more readily discovered when clinically-symptomatic rather than by radiographic surveillance.

Range area and the fast-slow continuum of life history traits predict pathogen richness in wild mammals.

Surveillance of pathogen richness in wildlife is needed to identify host species with a high risk of zoonotic disease spillover. While several predictors of pathogen richness in wildlife hosts have been proposed, their relative importance has not been formally examined. This hampers our ability to identify potential disease reservoirs, particularly in remote areas with limited surveillance efforts.

Indigenous Peoples' Lands are critical for safeguarding vertebrate diversity across the tropics.

Indigenous Peoples are long-term custodians of their lands, but only recently are their contributions to conservation starting to be recognized in biodiversity policy and practice. Tropical forest loss and degradation are lower in Indigenous lands than unprotected areas, yet the role of Indigenous Peoples' Lands (IPL) in biodiversity conservation has not been properly assessed from regional to global scales. Using species distribution ranges of 11,872 tropical forest-dependent vertebrates to create area of habitat maps, we identified the overlap of these species ranges with IPL and then compared values inside and outside of IPL for species richness, extinction vulnerability, and range-size rarity.

Evaluation of the diagnostic accuracy of the serological test for paratuberculosis in cattle according to tuberculosis status.

Background: Enzyme-linked immunosorbent assays (ELISAs) are the most widely used diagnostic tools in bovine paratuberculosis (bPTB) control. However, their diagnostic accuracy may be compromised by bovine tuberculosis (bTB) infection, as both diseases share diagnostic targets.

Methods: The bPTB and bTB infection status of 228 animals was determined using microbiological tissue culture as a reference test.

Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of complex.

Bovine tuberculosis (bTB) is a zoonotic disease and a global health problem that is subjected to obligatory eradication programs in the European Union. Microbiological culture is an imperfect technique for bTB diagnosis. This study aims to compare and validate two DNA isolation protocols and three different specific DNA targets, IS6110, IS4, and mpb70, to confirm complex (MTC) infection by real-time PCR directly from fresh tissue samples.

Analysis of the spatial distribution of the serve and the type of serve-return in elite table tennis. Sex differences.

Serve and return of the serve are among the most critical technical-tactical factors influencing performance in table tennis (TT). The present study aimed to analyse sex differences in the spatial distribution of serve and serve-return in elite TT players. A total of 48 elite TT players (men:  = 24; women:  = 24) participated in the investigation.

Land-use change from market responses to oil palm intensification in Indonesia.

Oil palm is a major driver of tropical deforestation. A key intervention proposed to reduce the footprint of oil palm is intensifying production to free up spare land for nature, yet the indirect land-use implications of intensification through market forces are poorly understood. We used a spatially explicit land-rent modeling framework to characterize the supply and demand of oil palm in Indonesia under multiple yield improvement and demand elasticity scenarios and explored how shifts in market equilibria alter projections of crop expansion.

Diagnostic performance of faecal and tissue multiplex qPCR IS900/F57 for the detection of Mycobacterium avium subspecies paratuberculosis in cattle.

Mycobacterium avium subspecies paratuberculosis (MAP) is responsible for bovine-paratuberculosis (bPTB), which causes high production losses in cattle. A cross-sectional study was conducted in 228 cattle to evaluate the validity and diagnostic utility of a multiplex real-time PCR (qPCR) on faecal and intestinal samples [ileocaecal valve (ICV) and ileocaecal lymph nodes (ICLN)], using intestinal tissue culture as a reference test. Based on the sensitivity, specificity, and likelihood ratios (LR) obtained, the diagnostic value of faecal qPCR for confirming MAP infection was moderate (sensitivity 50.

National Early Warning Score-2 for Identification of Patients with Intermediate-High-Risk Pulmonary Embolism.

Consensus statements have proposed the use of the National Early Warning Score 2 (NEWS2) to identify stable patients with acute pulmonary embolism (PE) and an intermediate-high risk of adverse outcomes. We aimed to externally validate NEWS2 and compare it to another predictive score (Bova). Using NEWS2 (cutoff ≥5 and ≥7) and the Bova score (cutoff >4), we classified patients as intermediate-high risk (vs.

Impact of supplementation with dihydroxylated vitamin D on performance parameters and gut health in weaned Iberian piglets under indoor/outdoor conditions.

Background: Vitamin D may improve innate antimicrobial response and the integrity of the intestinal mucosal barrier representing an alternative to antibiotics for improving pig health. Therefore, benefits of dietary supplementation with a product based on vitamin D metabolite-rich plant extracts were assessed in 252 purebred Iberian piglets for a period of 60 days. The study group received 1,25 dihydroxyvitamin D (1,25(OH)D) (100 ppm) in the conventional feed, which already included vitamin D (2000 IU in the starter and 1000 IU in the adaptation diets, respectively).

Climate co-benefits of tiger conservation.

Biodiversity conservation is increasingly being recognized as an important co-benefit in climate change mitigation programmes that use nature-based climate solutions. However, the climate co-benefits of biodiversity conservation interventions, such as habitat protection and restoration, remain understudied. Here we estimate the forest carbon storage co-benefits of a national policy intervention for tiger (Panthera tigris) conservation in India.

Turning Garlic into a Modern Crop: State of the Art and Perspectives.

Garlic is cultivated worldwide for the value of its bulbs, but its cultivation is challenged by the infertility of commercial cultivars and the accumulation of pathogens over time, which occurs as a consequence of vegetative (clonal) propagation. In this review, we summarize the state of the art of garlic genetics and genomics, highlighting recent developments that will lead to its development as a modern crop, including the restoration of sexual reproduction in some garlic strains. The set of tools available to the breeder currently includes a chromosome-scale assembly of the garlic genome and multiple transcriptome assemblies that are furthering our understanding of the molecular processes underlying important traits like the infertility, the induction of flowering and bulbing, the organoleptic properties and resistance to various pathogens.