Molecular investigation and infection patterns of seven viruses of honey bee (Apis mellifera L, 1758) populations from southeastern Morocco.

An epidemiological survey of honey bee viruses was conducted on 87 clinically healthy beehives located in southeastern Morocco. The sampled colonies were analyzed by reverse transcriptase (RT)-PCR / Real Time RT-qPCR with the aim of detecting and / or quantifying the following viruses: acute bee paralysis virus (ABPV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), sacbrood virus (SBV), black queen cell virus (BQCV), Kashmir bee virus (KBV) and Israeli acute paralysis virus (IAPV). With the exception of the last two of these viruses, all the other five were detected with different prevalence rates.

Genomic and epidemiological evidence for the emergence of a hybrid with unusual epidemiology in northern Italy.

(.) is one of the main causative agents of animal and human leishmaniasis across many endemic areas in South America, Europe, North Africa, and Asia. Despite its clinical significance, little is known about the genetic diversity of circulating in a given endemic area.

Molecular Typing of spp. Causing Tegumentary Leishmaniasis in Northeastern Italy, 2014-2020.

Tegumentary leishmaniasis (TL) is endemic but neglected in southern Europe. Therefore, this study aimed to analyze the strains causing TL cases in northeastern Italy, where an upsurge of TL cases has been observed in the last decade. Sections from 109 formalin-fixed and paraffin-embedded (FFPE) biopsies of skin and mucosal tissues were collected from TL cases in the selected area.

Wildlife Hosts of in a Re-Emerging Focus of Human Leishmaniasis, in Emilia-Romagna, Northeast Italy.

In the last decade, an upsurge of human leishmaniasis has been reported in the Emilia-Romagna region, Northeast Italy. Epidemiologic data have raised doubts about the role of dogs as the main reservoirs for . In the present study, a total of 1,077 wild animals were screened for DNA in earlobe and spleen samples from 2019 to 2022.

The European Pine Marten (Linnaeus, 1758) Is Autochthonous in Sicily and Constitutes a Well-Characterised Major Phylogroup within the Species (Carnivora, Mustelidae).

No molecular data are currently available for the Sicilian populations of the European pine marten , thus preventing any sound inference about its native or non-native status on the island, as well as the local phylogeography of the species. In order to investigate these issues, we sequenced two mtDNA markers in road-killed specimens collected in Sicily. Both markers consistently demonstrated the existence of a well-characterised Sicilian clade of the species, which is endemic to the island and constitutes the sister group of a clade including the Mediterranean and Central-North European major phylogroups of the European pine marten.

Circulation of ST103 in a Free Stall Italian Dairy Farm.

We report here on an outbreak of mastitis caused by Streptococcus agalactiae, or group B Streptococcus, in a northern Italy (Lombardy Region) free stall dairy farm. This outbreak was unusual because it occurred in a closed dairy herd and proved to be extremely difficult to resolve even after the application of the classical control procedures, which are specifically focused on the contagious nature of S. agalactiae.

Powdered Sugar Examination as a Tool for the Assessment of Infection Levels in Honey Bee Colonies.

American Foulbrood (AFB) is a contagious and severe brood disease of honey bees caused by the spore-forming bacterium . The identification of honey bee colonies infected by is crucial for the effective control of AFB. We studied the possibility of identifying the infection levels by in honey bee colonies through the examination of powdered sugar samples collected in the hives.

Corrigendum: American Foulbrood in the Czech Republic: ERIC II Genotype of Is Prevalent.

[This corrects the article DOI: 10.3389/fvets.2021.

Host preference and Leishmania infantum natural infection of the sand fly Phlebotomus perfiliewi in northern Italy.

The host preference of hematophagous insects is important in determining the cycle of pathogens that they potentially transmit; for example, sand flies are competent vectors of Leishmania parasites. In this work, we evaluated the host preference of sand flies collected in the Emilia-Romagna region of Italy in 2018 and 2019 in an area in which Leishmania infantum circulates actively. Out of about 30,000 sampled sand flies, we obtained 252 engorged females, which were processed to identify the sources of blood meals.

American Foulbrood in the Czech Republic: ERIC II Genotype of Is Prevalent.

American foulbrood (AFB) is a dangerous disease of honeybees () caused by the spore-forming bacterium . According to the ERIC (enterobacterial repetitive intergenic consensus) classification, five genotypes are distinguished, i.e.

Co-Circulation of Phleboviruses and Parasites in Sand Flies from a Single Site in Italy Monitored between 2017 and 2020.

Sand flies transmit , which is responsible for causing leishmaniasis, as well as many phleboviruses, including the human pathogenic Toscana virus. We screened sand flies collected from a single site between 2017 and 2020 for the presence of both phleboviruses and . The sand flies were sampled with attractive carbon dioxide traps and CDC light traps between May and October.

West Nile and Usutu Viruses' Surveillance in Birds of the Province of Ferrara, Italy, from 2015 to 2019.

West Nile (WNV) and Usutu (USUV) viruses are mosquito-borne flaviviruses. Thanks to their importance as zoonotic diseases, a regional plan for surveillance of Arboviruses was implemented in Emilia-Romagna in 2009. The province of Ferrara belongs to the Emilia-Romagna region, and it is an endemic territory for these viruses, with favorable ecological conditions for abundance of mosquitoes and wild birds.

Evidence of Common Isolates of in Bovines and Humans in Emilia Romagna Region (Northern Italy).

(group B , GBS) is one of the most important agents of bovine mastitis and causes remarkable direct and indirect economic losses to the livestock sector. Moreover, this species can cause severe human diseases in susceptible individuals. To investigate the zoonotic potential of , 203 sympatric isolates from both humans and cattle, isolated in the same time frame (2018) and in the same geographic area (Emilia Romagna region, Northern Italy), were characterized by molecular capsular typing (MCT), pilus island typing (PI), and multi-locus sequence typing (MLST).

Autochthonous Cases of Mucosal Leishmaniasis in Northeastern Italy: Clinical Management and Novel Treatment Approaches.

Mucosal leishmaniasis (ML) is a rare clinical variant of tegumentary leishmaniasis in Mediterranean Europe. Here we report on three autochthonous cases of head and neck ML in patients living in Northeastern Italy. Patients presented with non-specific, long-standing symptoms of upper respiratory tract involvement, mimicking other diseases.

Isolation and Molecular Typing of from in a Re-Emerging Focus of Leishmaniasis, Northeastern Italy.

Visceral leishmaniasis (VL) caused by is a public health threat in the Emilia-Romagna region, northeastern Italy, but its epidemiology has not been fully elucidated in this area. The objective of this study was to characterize infection in sand flies collected in a re-emerging focus of VL in the Bologna province. During the summer of 2016, 6114 sand flies were collected, identified, and tested for detection.

Genetic Characterization of Canine Adenovirus Type 1 Detected by Real-Time Polymerase Chain Reaction in an Oral Sample of an Italian Wolf ().

We report the detection of canine adenovirus type 1 DNA by real-time PCR technique in an oral sample of an Italian wolf (). Genetic characterization of the virus revealed a strict relationship with viruses detected in dogs (), wolves, and red foxes (), suggesting that transmission between wild animals and dogs had occurred.

Isolation of a Trypanosome Related to (Kinetoplastea: Trypanosomatidae) from (Diptera: Psychodidae).

The group includes several trypanosome species hardly distinguishable due to the lack of discriminating morphological characters. Trypanosomes belonging to this group have been isolated from different bovine, ovine, and cervids in Europe, Africa, Asia, and Americas. The principal vectors of the group are considered tabanid flies; however, is transmitted exclusively by sheep keds.

Multilocus microsatellite typing (MLMT) reveals host-related population structure in Leishmania infantum from northeastern Italy.

Background: Visceral leishmaniasis (VL) caused by Leishmania infantum is an ongoing health problem in southern Europe, where dogs are considered the main reservoirs of the disease. Current data point to a northward spread of VL and canine leishmaniasis (CanL) in Italy, with new foci in northern regions previously regarded as non-endemic.

Methodology/principal Findings: Multilocus microsatellite typing (MLMT) was performed to investigate genetic diversity and population structure of L.

The associations for separated parents in Italy: Their role for parents' well-being and coparenting.

After separation or divorce, people generally experience a deterioration of health, not only in terms of physical well-being but also in terms of emotional and social well-being. In addition, when separated, individuals are parents as well and they are concerned with the well-being of their children. The main task for separated parents is to maintain a parental alliance (coparenting) for the sake of their children's well-being.

Rise and fall of outbreak-specific clone inside endemic pulsotype of 4,[5],12:i:-; insights from high-resolution molecular surveillance in Emilia-Romagna, Italy, 2012 to 2015.

Background and aimEpidemiology of human non-typhoid salmonellosis is characterised by recurrent emergence of new clones of the pathogen over time. Some clonal lines of have shaped epidemiology of the disease at global level, as happened for serotype Enteritidis or, more recently, for 4,[5],12:i:-, a monophasic variant of serotype Typhimurium. The same clonal behaviour is recognisable at sub-serotype level where single outbreaks or more generalised epidemics are attributable to defined clones.