A roadmap for developing and engineering pulmonary fibrosis models.

Idiopathic pulmonary fibrosis (IPF) is a severe form of pulmonary fibrosis. IPF is a fatal disease with no cure and is challenging to diagnose. Unfortunately, due to the elusive etiology of IPF and a late diagnosis, there are no cures for IPF.

Interdisciplinary collaboration in critical care alarm research: A bibliometric analysis.

Background: Alarm fatigue in nurses is a major patient safety concern in the intensive care unit. This is caused by exposure to high rates of false and non-actionable alarms. Despite decades of research, the problem persists, leading to stress, burnout, and patient harm resulting from true missed events.

Osteopontin as a Biomarker in Chronic Kidney Disease.

Osteopontin (OPN) is a ubiquitously expressed protein with a wide range of physiological functions, including roles in bone mineralization, immune regulation, and wound healing. OPN has been implicated in the pathogenesis of several forms of chronic kidney disease (CKD) where it promotes inflammation and fibrosis and regulates calcium and phosphate metabolism. OPN expression is increased in the kidneys, blood, and urine of patients with CKD, particularly in those with diabetic kidney disease and glomerulonephritis.

Bridging the gap between health and safety performance and owner's satisfaction in construction projects adopting pro-environmental construction practices: role of economic performance.

The satisfaction of clients/owners, though very important as a determinant of construction project performance, has often been given very little attention in pro-environmental-related studies. To this end, this study's aim is to determine how owner's satisfaction (OS) can be realized via health and safety performance (HSP) and economic performance (EP) on construction projects that adopt pro-environmental construction practices (PCP) in Nigeria and to determine how the impact of HSP on OS can vary contingent on the level of EP. This study's aim was realized through data obtained from a survey of 249 construction projects and analyzed by adopting the partial least squares structural equation modeling (PLS-SEM) technique.

Changes in vineyard soil parameters after repeated application of organic-inorganic amendments based on spent mushroom substrate.

The changes of physicochemical and biochemical parameters of a silty loam (S1) and sandy loam (S2) vineyard soils added with spent mushroom substrate (SMS) or SMS composted with ophite (OF) as rock dust (SMS + OF) were studied. Two doses of SMS or SMS + OF (25 and 100 Mg ha) were applied for two consecutive years (2020-2021) and changes of soil physicochemical parameters, and dehydrogenase activity (DHA), respiration (RES), microbial biomass (BIO), and the phospholipid fatty acids (PLFAs) profile were assayed on a temporal basis. The results showed an increase in soil organic carbon (OC) content, total and mineralised N, P, and K, especially when the highest SMS dose was applied to soils.

Control Strategy of an Underactuated Underwater Drone-Shape Robot for Grasping Tasks.

In underwater environments, ensuring people's safety is complicated, with potentially life-threatening outcomes, especially when divers have to work in deeper conditions. To improve the available solutions for working with robots in this kind of environment, we propose the validation of a control strategy for robots when taking objects from the seabed. The control strategy proposed is based on acceleration feedback in the model of the system.

Theoretical Study at the Molecular Mechanics Level of the Interaction of Tetracycline and Chloramphenicol with the Antibiotic Receptors Present in (Q839F7) and (Q8DS20).

When dental infections occur, various types of antibiotics are used to combat them. The most common antibiotics to be used are tetracycline and chloramphenicol; likewise, the most common bacteria in dental infections are and . In the present work, we have studied by molecular mechanics methods the interactions of the ribosomal proteins L16 present in and , identified with UNIPROT code Q839F7 and Q8DS20, respectively.

Vitamin D3 Repletion Improves Vascular Function, as Measured by Cardiorenal Biomarkers in a High-Risk African American Cohort.

Background: 25-hydroxy vitamin D (Vit D)-deficiency is common among patients with chronic kidney disease (CKD) and contributes to cardiovascular disease (CVD). African Americans (AAs) suffer disproportionately from CKD and CVD, and 80% of AAs are Vit D-deficient. The impact of Vit D repletion on cardio-renal biomarkers in AAs is unknown.

Alcohol availability during withdrawal gates the impact of alcohol vapor exposure on responses to alcohol cues.

Rationale: Chronic intermittent ethanol (CIE) vapor inhalation is a widely used model of alcohol dependence, but the impact of CIE on cue-elicited alcohol seeking is poorly understood.

Objective: Here, we assessed the effects of CIE on alcohol-seeking elicited by cues paired with alcohol before or after CIE vapor inhalation.

Methods: In experiment 1, male and female Long-Evans rats were trained in a discriminative stimulus (DS) task, in which one auditory cue (the DS) predicts the availability of 15% ethanol and a control cue (the NS) predicts no ethanol.

A Robust Protocol for Decellularized Human Lung Bioink Generation Amenable to 2D and 3D Lung Cell Culture.

Decellularization efforts must balance the preservation of the extracellular matrix (ECM) components while eliminating the nucleic acid and cellular components. Following effective removal of nucleic acid and cell components, decellularized ECM (dECM) can be solubilized in an acidic environment with the assistance of various enzymes to develop biological scaffolds in different forms, such as sheets, tubular constructs, or three-dimensional (3D) hydrogels. Each organ or tissue that undergoes decellularization requires a distinct and optimized protocol to ensure that nucleic acids are removed, and the ECM components are preserved.

BOK controls apoptosis by Ca transfer through ER-mitochondrial contact sites.

Calcium transfer from the endoplasmic reticulum (ER) to mitochondria is a critical contributor to apoptosis. B cell lymphoma 2 (BCL-2) ovarian killer (BOK) localizes to the ER and binds the inositol 1,4,5-trisphosophate receptor (IP3R). Here, we show that BOK is necessary for baseline mitochondrial calcium levels and stimulus-induced calcium transfer from the ER to the mitochondria.

Omental free flap for surgical treatment of chronic osteomyelitis of lower limb: A technical note.

Soft tissue reconstruction of chronic lower extremity wounds with bone infection entails an important challenge in reconstructive surgery. We report our experience using the omentum free flap to provide coverage in two patients suffering chronic osteomyelitis of the lower limbs. After extensive soft tissue and bone debridement, an omentum free flap was performed in both cases, providing dead space obliteration and soft tissue coverage in behalf of its large size and pliability.

Field measurement and modelling of chlorotoluron and flufenacet persistence in unamended and amended soils.

The dissipation and persistence of two cereals herbicides, chlorotoluron and flufenacet, were studied in a field experiment including three replicated plots of unamended soil (S), soil amended with spent mushroom substrate (S + SMS), and soil amended with green compost (S + GC), during the winter wheat cultivation campaign. The SMS and GC organic residues were applied to the soil at rates of 140 or 85 t residue ha, and herbicides were sprayed as Erturon® and Herold® formulations for chlorotoluron and flufenacet, respectively. Concentrations of both herbicides and of their metabolites were regularly measured in the three soil treatments (0-10 cm) from 0 to 339 days.

Mobility monitoring of two herbicides in amended soils: A field study for modeling applications.

This paper reports the mobility and total balance of chlorotoluron (CTL), flufenacet (FNC) and bromide ion (Br) throughout a sandy soil profile after the application of spent mushroom substrate (SMS) and green compost (GC). Obtaining mobility dataset is crucial to simulate the herbicides' fate under amended soil scenarios by application pesticide leaching models with regulatory application (FOCUS models). The application of organic residues is nowadays increased to improve the crop yields and there is a gap in the simulations of this kind of amended scenarios.

Modelling herbicides mobility in amended soils: Calibration and test of PRZM and MACRO.

Addition of organic residues to soil is a current farming practice but it is not considered in the modelling studies for pesticide risk assessment at regulatory level despite its potential impact on the pesticide dynamics in soil. Thus, the objective of this work was to examine and to compare the ability of PRZM and MACRO pesticide fate models to simulate soil water content, and bromide (Br, tracer), chlorotoluron and flufenacet concentrations in the soil profiles (0-100 cm) of one agricultural soil, unamended (control soil, S), amended with spent mushroom substrate (S + SMS) or amended with green compost (S + GC). Based on a two-year field-scale dataset, the models were first calibrated against measurements of water and solutes contents in the soil profiles (first year) and then tested without any further model calibration by comparison with the field observations of the second year.

Dataset on solar contributions by thermal solar systems in Chile applying Chilean and Spanish regulations.

The data presented in this article are related to the research article entitled "Solar domestic hot water regulation in the Latin American residential sector with the implementation of the Energy Performance of Buildings Directive: The case of Chile" (López-Ochoa et al., 2019), which evaluates the possibility of adapting Spanish solar domestic hot water regulations in Chile, with the objective of presenting the potential impact of the Energy Performance of Buildings Directive in Latin America. This dataset was made publicly available to show the possible energy savings with the thermal solar systems proposed to enable the use of these data by other researchers as well as designers, installers and decision-makers.

X-ray Determination of Compressive Residual Stresses in Spring Steel Generated by High-Speed Water Quenching.

Automotive components manufacturers use the 5160 steel in leaf and coil springs. The industrial heat treatment process consists in austenitizing followed by the oil quenching and tempering process. Typically, compressive residual stresses are induced by shot peening on the surface of automotive springs to bestow compressive residual stresses that improve the fatigue resistance and increase the service life of the parts after heat treatment.

Previous degradation study of two herbicides to simulate their fate in a sandy loam soil: Effect of the temperature and the organic amendments.

A laboratory study was designed to assess the following: i) the degradation kinetics of chlorotoluron and flufenacet at two different temperatures, 6 °C and 16 °C, in an unamended agricultural soil and one amended with spent mushroom substrate (SMS) and green compost (GC), and ii) the formation of the main metabolites of both herbicides with potential risk for water pollution over degradation time. The aim was to determine the dependence of these herbicide degradations on temperature (Q factor) using kinetic parameters, which is essential information for the later simulation of herbicide environmental fate with FOCUS models. SMS and GC were applied in situ to the natural soil as organic amendments at rates of 140 or 85 t residue ha, respectively.

Methods to Probe Calcium Regulation by BCL-2 Family Members.

BCL-2 family members have additional roles beyond direct regulation of mitochondrial outer membrane permeabilization (MOMP) in apoptosis. One such important function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.

Cable-Driven Parallel Robot with Reconfigurable End Effector Controlled with a Compliant Actuator.

Redundancy in cable-driven parallel robots provides additional degrees of freedom that can be used to achieve different objectives. In this robot, this degree of freedom is used to act on a reconfigurable end effector with one degree of freedom. A compliant actuator actuated by one motor exerts force on both bodies of the platform.