Publications by authors named "Carolyn M McCormick"
Article Synopsis
- Tissue clearing combined with light-sheet microscopy (LSFM) allows for high-resolution imaging of entire mouse brains, revealing structural changes related to genetic or environmental factors that traditional microscopy may overlook.
- This study introduces "NuMorph," a set of tools that efficiently quantifies all nuclei in postnatal mouse brains, enhancing data analysis beyond just counting sparse cell populations.
- By utilizing publicly available protocols and software, researchers can easily apply these advanced imaging and analysis techniques to better understand brain organization and cell-type distribution.
Background: Recent advances in tissue clearing techniques, combined with high-speed image acquisition through light sheet microscopy, enable rapid three-dimensional (3D) imaging of biological specimens, such as whole mouse brains, in a matter of hours. Quantitative analysis of such 3D images can help us understand how changes in brain structure lead to differences in behavior or cognition, but distinguishing densely packed features of interest, such as nuclei, from background can be challenging. Recent deep learning-based nuclear segmentation algorithms show great promise for automated segmentation, but require large numbers of accurate manually labeled nuclei as training data.
View Article and Find Full Text PDFPurpose: Patient presentation after brachial plexus birth injury (BPBI) is influenced by nerve injury location; more contracture and bone deformity occur at the shoulder in postganglionic injuries. Although bone deformity after postganglionic injury is well-characterized, the extent of glenohumeral deformity after preganglionic BPBI is unclear.
Methods: Twenty Sprague-Dawley rat pups received preganglionic or postganglionic neurectomy on a single forelimb at postnatal days 3 to 4.
Article Synopsis
- Brachial plexus birth injuries vary in symptoms based on whether the nerve is torn close to or far from the spinal cord, with more severe shoulder issues present in cases of postganglionic injuries.
- An experiment with 17 rats examined muscle changes after the rats underwent nerve cut surgeries, measuring various aspects such as range of motion and muscle structure after 8 weeks.
- Results showed significant differences in muscle growth and mass loss between preganglionic and postganglionic injuries, indicating that contractures are influenced not only by limited muscle growth but also by the extent of muscle mass reduction following an injury.