Publications by authors named "Caroline P de Bortoli"

The resistance of pest insects to biopesticides based on the bacterium Bacillus thuringiensis (Bt) is normally associated with changes to the receptors involved in the mechanism of action of the pesticidal proteins produced by Bt. In some strains of Plutella xylostella (the diamondback moth) resistance has evolved through a signalling mechanism in which the genes encoding the receptor proteins are downregulated whereas in others it has been linked to structural changes in the receptors themselves. One such well characterized mutation is in the ABCC2 gene indicating that changes to this protein can result in resistance.

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The fall armyworm (Spodoptera frugiperda) is a major global pest causing severe damage to various crops, especially corn. Transgenic corn producing the Cry1F pesticidal protein from the bacterium Bacillus thuringiensis (Cry1F corn) showed effectiveness in controlling this pest until S. frugiperda populations at locations in North and South America evolved practical resistance.

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The fall armyworm (JE Smith) () is a polyphagous pest targeted by selected Cry and Vip3A insecticidal proteins from the bacterium (Bt) that are produced in transgenic Bt corn and cotton. Available evidence suggests that sublethal larval exposure to Cry1Ac increases flight activity in adult spp. However, it is not known whether this effect is also observed in survivors from generally lethal exposure to Cry1Ac.

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Article Synopsis
  • The evolution of resistance in pests poses a significant risk to the effectiveness and sustainability of Bt crops that produce insecticidal proteins.
  • Monitoring for resistance to Cry and Vip3A proteins is essential to prevent further resistance development, with traditional methods involving bioassays currently in use.
  • The study demonstrates that targeted DNA sequencing can effectively identify known and new resistance alleles in moth populations, improving monitoring efforts and revealing resistance alleles common to populations in different regions.
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Article Synopsis
  • The fall armyworm is a major agricultural pest affecting food security globally, recognized for its diverse diet and migratory behavior, with distinct strains associated with corn and rice.
  • This study analyzed the genetic diversity of 55 fall armyworm samples from various countries, revealing a largely interconnected population in the USA with small differences tied to host strains, and found evidence of multiple introductions from the Eastern Hemisphere.
  • This research represents a significant advancement in understanding the population genomics of the fall armyworm in the USA and supports the use of laboratory strains for studying genetic differences, while highlighting the complexities of host strain interactions.
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Bacillus thuringiensis (Bt) biopesticides are an environmentally safe alternative to the management of Plutella xylostella pesticide resistance evolution. We evaluated P. xylostella susceptibility to six Bt strains cultivated and applied individually, and 15 combinations of Bt strains mixed after cultivation.

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Bacteria represent the most commercially successful entomopathogenic microbial group, with most commercialized insecticides containing gram-positive bacteria in the Bacillaceae family. Resistance to entomopathogenic bacteria threatens sustainable agriculture, and information on the mechanisms and genes involved is vital to develop management practices aimed at reducing this risk. We provide an integrative summary on mechanisms responsible for resistance to commercialized entomopathogenic bacteria, including information on resistance to transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt crops).

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In Brazil, the parasitoid Cotesia flavipes (Cameron) has been used as a biological control agent against Diatraea saccharalis (F.) since 1976, because of its effectiveness in reducing populations of this pest. Three million plastic cups are used annually to release C.

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Hemotrophic mycoplasmas and Bartonella species are important pathogens that circulate between cats and invertebrate hosts, occasionally causing diseases in humans. Nevertheless, there are few reports on occurrences of these agents in cats in Brazil. The present study aimed to detect the presence of hemoplasma and Bartonella DNA by means of PCR and sequencing.

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Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids. The present work aimed to characterize molecularly the presence of Hepatozoon sp. DNA in cat blood samples from São Luís Island, Maranhão state, Northeastern Brazil.

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