Food swamps in Campinas, Brazil.

The characteristics of the food environment can interfere with physical access to healthy foods and accentuate health inequalities. The presence of food swamps, i.e.

Nutritional status and clinical evolution of the elderly in home enteral nutritional therapy: a retrospective cohort study.

Objective: to evaluate the clinical and nutritional evolution of elderly patients receiving home enteral nutritional therapy.

Method: retrospective cohort observational study. Data collection was performed through the analysis of clinical and nutritional records.

Different assay conditions for detecting the production and release of heat-labile and heat-stable toxins in enterotoxigenic Escherichia coli isolates.

Enterotoxigenic Escherichia coli (ETEC) produce heat-labile (LT) and/or heat-stable enterotoxins (ST). Despite that, the mechanism of action of both toxins are well known, there is great controversy in the literature concerning the in vitro production and release of LT and, for ST, no major concerns have been discussed. Furthermore, the majority of published papers describe the use of only one or a few ETEC isolates to define the production and release of these toxins, which hinders the detection of ETEC by phenotypic approaches.

Metabolic profile of clinically severe obese patients.

Background: Since low basal metabolic rate (BMR) is a risk factor for weight regain, it is important to measure BMR before bariatric surgery. We aimed to evaluate the BMR among clinically severe obese patients preoperatively. We compared it with that of the control group, with predictive formulas and correlated it with body composition.

Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli.

Strains of enterotoxigenic Escherichia coli (ETEC) are responsible for significant rates of morbidity and mortality among children, particularly in developing countries. The majority of clinical and public health laboratories are capable of isolating and identifying Salmonella, Shigella, Campylobacter, and Escherichia coli O157:H7 from stool samples, but ETEC cannot be identified by routine methods. The method most often used to identify ETEC is polymerase chain reaction for heat-stable and heat-labile enterotoxin genes, and subsequent serotyping, but most clinical and public health laboratories do not have the capacity or resources to perform these tests.

Exploratory study of some acoustic and articulatory characteristics of sad speech.

This study examines acoustic and articulatory EMA data of two female speakers (American and Japanese) spontaneously producing emotional speech while engaged in an informal telephone-type conversation. A set of control data in which the speakers imitated or read the original emotional utterance was also recorded; for the American speaker, the intonation pattern was also imitated. The results suggest (1) acoustic and articulatory characteristics of spontaneous sad speech differ from that of read speech or imitated intonation speech, (2) spontaneous sad speech and imitated sad speech seem to have similar acoustic characteristics (high F(0), changed F(1) as well as voice quality), but articulation is different in terms of lip, jaw and tongue positions, and (3) speech that is rated highly by listeners as sad is associated with high F(0) and changed voice quality.

Inhibition of Escherichia coli heat-labile enterotoxin by neoglycoprotein and anti-lectin antibodies which mimic GM1 receptor.

Escherichia coli producing heat-labile enterotoxin is responsible for numerous cases of diarrhea worldwide, leading to considerable morbidity and mortality. The B subunits of this toxin are responsible for the binding to the receptor, the complex ganglioside GM1 which has galactose as its terminal sugar. In this study we showed that analogs of galactose (gal) and N-acetylgalactosamine (GalNAc) interfere with the binding of heat-labile toxin to GM1.