Publications by authors named "Carolina Leguina"
Article Synopsis
- This study investigates a multidrug-resistant strain of E. coli that produces Klebsiella pneumoniae carbapenemase (KPC), which poses a serious public health threat due to its genetic features and resistance.
- Whole-genome sequencing and various bioinformatics tools were utilized to analyze the strain's resistance genes, plasmids, and virulence factors, leading to the identification of sequence type ST648 as previously unreported for this context in Latin America.
- The findings emphasize the need for ongoing molecular surveillance to track the spread of this high-risk clone and its associated antibiotic resistance, highlighting the potential for greater global dissemination.
Objectives: Isolation of colistin- and carbapenem-resistant Klebsiella pneumoniae (CCR-Kp) is increasing in hospital settings worldwide, which is related to increased morbidity, mortality and healthcare costs. The aim of this work was to perform whole-genome sequencing (WGS), genomic and phylogenetic analysis, and conjugation assays of an extensively drug-resistant (XDR) CCR-Kp isolate from Argentina.
Methods: WGS of strain KpS26 isolated from a bloodstream infection was performed using Illumina MiSeq-I, and de novo assembly was achieved using SPAdes v.