The tobacco GNTI stem region harbors a strong motif for homomeric protein complex formation.

Introduction: The Golgi apparatus of plants is the central cellular organelle for glycan processing and polysaccharide biosynthesis. These essential processes are catalyzed by a large number of Golgi-resident glycosyltransferases and glycosidases whose organization within the Golgi is still poorly understood.

Methods: Here, we examined the role of the stem region of the /medial Golgi enzyme N-acetylglucosaminyltransferase I (GNTI) in homomeric complex formation in the Golgi of using biochemical approaches and confocal microscopy.