Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Is Required in for Efficient Soybean Root Colonization and Competition for Nodulation.

The Hyphomicrobiales (Rhizobiales) order contains soil bacteria with an irregular distribution of the Calvin-Benson-Bassham cycle (CBB). Key enzymes in the CBB cycle are ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), whose large and small subunits are encoded in and , and phosphoribulokinase (PRK), encoded by . These genes are often found in operons, regulated by the LysR-type regulator CbbR.

Induction by of Different Pathways for Growth in D-mannitol or L-arabinose Leading to Pronounced Differences in CO Fixation, O Consumption, and Lateral-Flagellum Production.

, a soybean N-fixing symbiont, constitutes the basic input in one of the most prominent inoculant industries worldwide. This bacterium may be cultured with D-mannitol or L-arabinose as carbon-plus-energy source (C-source) with similar specific growth rates, but with higher biomass production with D-mannitol. To better understand the bacterium's carbon metabolism, we analyzed, by liquid chromatography and tandem mass spectrometry (MS), the whole set of proteins obtained from cells grown on each C-source.