Publications by authors named "Carolina Backman Johansson"
Article Synopsis
- Hibernation triggers are not fully understood, but there are metabolic similarities to sleep, which may be influenced by n-3 fatty acids in humans.
- The study analyzed plasma fatty acid profiles in free-ranging brown bears and captive garden dormice, focusing on their differing hibernation patterns and dietary fatty acid concentrations.
- Results showed distinct changes in fatty acids during hibernation, with both species experiencing decreased alpha-linolenic acid and eicosapentaenoic acid, while n-3 docosapentaenoic acid increased; suggesting a potential link between fatty acid metabolism and hibernation that warrants more research.
Background: Measurements of pleural fluid biomarkers for rapid identification of complicated parapneumonic effusion (CPPE) are crucial for optimal management. Previous studies for biomarker evaluation were however based on pleura culture, not modern DNA technique. Lactate has not been thoroughly studied earlier as a potential biomarker in this regard.
View Article and Find Full Text PDFIntegration of whole genome sequencing into a healthcare setting: high diagnostic rates across multiple clinical entities in 3219 rare disease patients.
Genome Med
March 2021
Article Synopsis
- The study analyzes whole genome sequencing data from 4,437 individuals (3,219 patients and 1,218 relatives) at the Genomic Medicine Center Karolinska-Rare Diseases to advance genomics-based diagnostics in Stockholm's healthcare system.
- Results show that 40% of patients received a molecular diagnosis, with variation in detection rates among different disease groups and involving 754 different causative genes.
- The initiative also contributes to research by sharing data internationally, leading to the discovery of 17 new disease-causing genes and fostering collaboration among expert teams for complex cases.
Sweden has one neonatal screening laboratory, receiving 115 to 120 thousand samples per year. Among the one million babies screened by tandem mass spectrometry from November 2010 until July 2019, a total of 665 babies were recalled and 311 verified as having one of the diseases screened for with this methodology, giving a positive predictive value (PPV) of 47% and an incidence of 1:3200. The PPV was high (41%) already in the first year after start of screening, thanks to the availability of the collaborative project Region 4 Stork database.
View Article and Find Full Text PDFBackground: The most clinically useful blood ketone in the diagnosis, management, and recovery of diabetes ketoacidosis in both adults and children is 3-hydroxybutyrate. In the absence of laboratory routine methods, several point-of-care methods are in use, but very few clinical evaluations are published.
Methods: This study evaluates linearity and reproducibility of two handheld point-of-care meters for blood 3-hydroxybutyrate measurement for hospital use, Nova StatStrip, and FreeStyle Precision Pro.
The aim of this study was to use a 16S rDNA sequencing method in combination with conventional culture in patients with parapneumonic effusions (PPE) to evaluate the methods, study the microbiological spectrum, and examine the presence of bacteria within the different stages of PPE. Adults with community-acquired pneumonia (CAP) and PPE (n = 197) admitted to the Departments of Infectious Diseases at four hospitals in Stockholm County during 2011-2014 were prospectively studied. All patients underwent thoracentesis.
View Article and Find Full Text PDFSickle Cell Disease (SCD) is an increasing global health problem and presents significant challenges to European health care systems. Newborn screening (NBS) for SCD enables early initiation of preventive measures and has contributed to a reduction in childhood mortality from SCD. Policies and methodologies for NBS vary in different countries, and this might have consequences for the quality of care and clinical outcomes for SCD across Europe.
View Article and Find Full Text PDFWe studied procalcitonin (PCT) levels at hospital admittance and their association with aetiology and severity in patients with community-acquired pneumonia (CAP). Median PCT concentrations were higher in bacteraemic patients than in those without bacteraemia (6.11 μg/L vs 0.
View Article and Find Full Text PDFBackground: Self-monitoring of blood glucose is a cornerstone of diabetes management. The aim of this study was to evaluate the analytical quality and the ease of use of the Accu-Chek Mobile, a new glucose monitoring system designed for capillary blood testing by diabetic patients.
Materials And Methods: The performance of the Accu-Chek Mobile was evaluated both in the hands of a scientist and of diabetes patients.