Publications by authors named "Carolin B Wagner"
Article Synopsis
- At critically short telomeres, TERRA RNA-DNA hybrids play a role in promoting homology-directed repair (HDR), which helps delay cellular aging (replicative senescence).
- Even in longer telomeres, transient TERRA RNA-DNA hybrids form, suggesting they may have additional regulatory functions, such as preventing harmful DNA resection when telomeres lose their functionality.
- Experimental evidence using the cdc13-1 allele shows that stabilizing or destabilizing these hybrids influences single-stranded DNA (ssDNA) generation, impacting cell viability while not affecting the overall rate of telomere shortening.
Cancer cells achieve immortality by employing either homology-directed repair (HDR) or the telomerase enzyme to maintain telomeres. ALT (alternative lengthening of telomeres) refers to the subset of cancer cells that employ HDR. Many ALT features are conserved from yeast to human cells, with the yeast equivalent being referred to as survivors.
View Article and Find Full Text PDFIt has recently been demonstrated that budding yeast telomeres are transcribed into TERRA, a long noncoding RNA. Due to the G-rich nature of the coding strand, TERRA has a tendency to form DNA-RNA hybrids and potentially R-loops, which in turn, promote repair at short telomeres. Here, we report two methods to detect DNA-RNA hybrids at yeast telomeres, namely, DRIP, which employs the S9.
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