A new HPLC method with multiple detection systems for impurity analysis and discrimination of natural versus synthetic cannabidiol.

Cannabidiol (CBD) is the main non-psychoactive phytocannabinoid derived from Cannabis sativa L. It is now an active pharmaceutical ingredient (API), given its usage in treating some types of pediatric epilepsy. For this reason, this compound requires a deep characterization in terms of purity and origin.

Extraction, purification and assessment of the antioxidant and anti-inflammatory activity of policosanols from non-psychoactive L.

Policosanols (PCs) are bioactive compounds extracted from different natural waxes. In this work, the purification, characterization and assessment of the antioxidant and anti-inflammatory activity was carried out on PCs from an innovative source, i.e.

A label free chemoproteomic-based platform to disclose cannabidiol molecular mechanism of action on chronic myelogenous leukemia cancer cells.

The discovery of the interactome of cannabidiol (CBD), a non-psychoactive cannabinoid from L., has been here performed on chronic myelogenous leukemia cancer cells, using an optimized chemo-proteomic stage, which links Drug Affinity Responsive Target Stability with Limited Proteolysis Multiple Reaction Monitoring approaches. The obtained results showed the ability of CBD to target simultaneously some potential protein partners, corroborating its well-known poly-pharmacology activity.

Identification of phenolic compounds from inflorescences of non-psychoactive Cannabis sativa L. by UHPLC-HRMS and in vitro assessment of the antiproliferative activity against colorectal cancer.

Phenolic compounds from Cannabis sativa L. (Cannabaceae family), in particular cannflavins, are known to possess several biological properties. However, their antiproliferative activity, being of great interest from a medicinal chemistry point of view, has not been deeply investigated so far in the literature.

Helichrysum stoechas (L.) Moench reduces body weight gain and modulates mood disorders via inhibition of silent information regulator 1 (SIRT1) by arzanol.

The prevalence of obesity is steadily rising, making safe and more efficient anti-obesity treatments an urgent medical need. Growing evidence correlates obesity and comorbidities, including anxiety and depression, with the development of a low-grade inflammation in peripheral and central tissues. We hypothesized that attenuating neuroinflammation might reduce weight gain and improve mood.

An innovative method for the extraction and HPLC analysis of bioactive policosanols from non-psychoactive Cannabis sativa L.

Policosanols (PCs) refer to a mixture of long-chain aliphatic alcohols. Sugar cane is the main industrial source of PCs, but others, including beeswax and Cannabis sativa L., are also known.

Application of experimental design in HPLC method optimisation for the simultaneous determination of multiple bioactive cannabinoids.

The scientific interest in Cannabis sativa L. analysis has been rapidly increasing in recent years, especially for what concerns cannabinoids, plant secondary metabolites which are well known for having many biological properties. High-performance liquid chromatography (HPLC) is frequently used for both the qualitative and quantitative analysis of cannabinoids in plant extracts from C.

Key role of retardation and non-locality in sound propagation in amorphous solids as evidenced by a projection formalism.

We investigate acoustic propagation in amorphous solids by constructing a projection formalism based on separating atomic vibrations into two, "phonon" (P) and "non-phonon" (NP), subspaces corresponding to large and small wavelengths. For a pairwise interaction model, we show the existence of a "natural" separation lengthscale, determined by structural disorder, for which the isolated P subspace presents the acoustic properties of a nearly homogenous (Debye-like) elastic continuum, while the NP one encapsulates all small scale non-affinity effects. The NP eigenstates then play the role of dynamical scatterers for the phonons.

[Myocardial ischemia without coronary obstructions: MINOCA-INOCA. Review for decision making].

One in every four coronarographies performed to study myocardial ischemia shows coronary angiographic stenosis less than 50%. This data triggered an increasing interest in the medical community. The American Society of Cardiology recently published a position paper about the pathophysiology, diagnosis and treatment of this entity.

[Argentine Presidential Medical Unit. Survey of the Argentine hospital network and resources for golden hour pathologies.].

Introduction: Presidential medical units are intended to protect the dignitary's health in multiple aspects and work in close relationship with security. There are three central areas of coverage: myocardial infarction, stroke and trauma. By 2016 we had not found information about the resources on medical centers in Argentina and their integration into healthcare networks.

Fluctuating Elasticity Fails to Capture Anomalous Sound Scattering in Amorphous Solids.

The fluctuating elasticity (FE) model, introduced phenomenologically and developed by Schirmacher [J. Non-Cryst. Solids 357, 518 (2011)JNCSBJ0022-309310.

Glass-like stress relaxation of a permanent gelatin network as a signature of pretransitional helix-coil fluctuations.

The stress response of permanently crosslinked gelatin gels was recently observed to display glass-like features, namely, a stretched-exponential behavior terminated by an exponential decay, the characteristic time scales of which increase dramatically with decreasing temperature. This phenomenon is studied here using a model of flexible polymer gel network where relaxation proceeds via elementary monomer exchanges between helix and coil segments. The relaxation dynamics of a full network simulation is found to be nearly identical to that of a model of independent strands, which shows that for flexible polymer gels in the range of elastic moduli of interest, both strand contour length disorder and elastic couplings are irrelevant.

Preferential hydration fully controls the renaturation dynamics of collagen in water-glycerol solvents.

Glycerol is one of the additives which stabilize collagen, as well as globular proteins, against thermally induced denaturation --an effect explained by preferential hydration, i.e. by the formation, in water/glycerol solvents, of a hydration layer whose entropic cost favors the more compact triple-helix native structure against the denatured one, gelatin.

Effects of disorder and chain stiffening on the elasticity of flexible polymer networks.

We examine how the distribution of contour lengths and the high-stretch stiffening of individual chain segments affect the macroscopic shear modulus of flexible polymer gels, using a two-dimensional numerical model in which polymer segments form a triangular network and disorder is introduced by varying their contour lengths. We show that, in the relevant parameter range: (i) the nonaffine contribution to the shear modulus is negligible, i.e.

Glass-like dynamics of the strain-induced coil/helix transition on a permanent polymer network.

We study the stress response to a step strain of covalently bonded gelatin gels in the temperature range where triple helix reversible crosslink formation is prohibited. We observe slow stress relaxation towards a T-dependent finite asymptotic level. We show that this is assignable to the strain-induced coil → helix transition, previously evidenced by Courty et al.

Estimation of the cost-effectiveness of apixaban versus vitamin K antagonists in the management of atrial fibrillation in Argentina.

Apixaban, a novel oral anticoagulant which has been approved for the prevention of stroke and systemic embolism in non-valvular atrial fibrillation, reduces both ischemic and haemorrhagic stroke and produces fewer bleedings than vitamin K antagonist warfarin. These clinical results lead to a decrease in health care resource utilization and, therefore, have a positive impact on health economics of atrial fibrillation. The cost-effectiveness of apixaban has been assessed in a variety of clinical settings and countries.

Two-step build-up of a thermoreversible polymer network: From early local to late collective dynamics.

We probe the mechanisms at work in the build-up of thermoreversible gel networks, with the help of hybrid gelatin gels containing a controlled density of irreversible, covalent crosslinks (CLs), which we quench below the physical gelation temperature. The detailed analysis of the dependence on covalent crosslink density of both the shear modulus and optical activity evolutions with time after quench enables us to identify two stages of the physical gelation process, separated by a temperature-dependent crossover modulus: (i) an early nucleation regime during which rearrangements of the triple-helix CLs play a negligible role, and (ii) a late, logarithmic aging one, which is preserved, though slowed down, in the presence of irreversible CLs. We show that aging is fully controlled by rearrangements and discuss the implication of our results in terms of the switch from an early, local dynamics to a late, cooperative long-range one.

Ultrafast spherulitic crystal growth as a stress-induced phenomenon specific of fragile glass-formers.

We propose a model for the abrupt emergence, below temperatures close to the glass transition, of the ultrafast (glass-to-crystal) steady mode of spherulitic crystal growth in deeply undercooled liquids. We interpret this phenomenon as controlled by the interplay between the generation of stresses by crystallization and their partial release by flow in the surrounding amorphous visco-elastic matrix. Our model is consistent with both the observed ratios (∼10(4)) of fast-to-slow velocities and the fact that fast growth emerges close to the glass transition.

[Contrast induced nephropathy in acute coronary syndrome].

Contrast induced nephropathy (CIN) is one of the most frequent causes of acute renal failure in hospitalized patients. It is associated with an increase in morbidity and mortality in patients hospitalized for acute coronary syndrome (ACS) undergoing percutaneous coronary intervention (PCI). Risk factors and prevention strategies are not well defined.

Robustness of avalanche dynamics in sheared amorphous solids as probed by transverse diffusion.

Using numerical simulations, we perform an extensive finite-size analysis of the transverse diffusion coefficient in a sheared 2D amorphous solid over a broad range of strain rates at temperatures up to the supercooled liquid regime. We thus obtain direct qualitative evidence for the persistence of correlations between elementary plastic events up to the vicinity of the glass transition temperature T(g). A quantitative analysis of the data, combined with a previous study of the T and γ dependence of the macroscopic stress [Phys.

Microstructuration stages during gelation of gelatin under shear.

We study gelation under shear of aqueous gelatin by measuring the evolution of the apparent viscosity, thus extending the previous study by de Carvalho and Djabourov (W. de Carvalho, M. Djabourov, Rheol.

Pharmacogenomics and adverse drug reactions: the case of statins.

Introduction: The use of genomics to predict adverse drug reactions (ADRs) has been the subject of much research over the last decade. Concerns about the muscular safety of statins, a highly prescribed group of drugs, are partially related to their high exposure. Many studies have identified a variety of genetic markers related to statin-induced myopathy.

Universal additive effect of temperature on the rheology of amorphous solids.

Extensive measurements of macroscopic stress in a 2D Lennard-Jones glass, over a broad range of temperatures (T) and strain rates (γ), demonstrate a very significant decrease of the flowing stress with T, even much below the glass transition. A detailed analysis of the interplay between loading, thermal activation, and mechanical noise leads us to propose that over a broad (γ, T) region, the effect of temperature amounts to a mere lowering of the strains at which plastic events occur, while the athermal avalanche dynamics remains essentially unperturbed. Up to the vicinity of the glass transition, temperature is then shown to correct the athermal stress by a (negative) additive contribution which presents a universal form, thus bringing support to and extending an expression proposed by Johnson and Samwer [Phys.

Interplay between shear loading and structural aging in a physical gelatin gel.

We show that the aging of the mechanical relaxation of a gelatin gel exhibits the same scaling phenomenology as polymer and colloidal glasses. In addition, gelatin is known to exhibit logarithmic structural aging (stiffening). We find that stress accelerates this process.

Rate-dependent avalanche size in athermally sheared amorphous solids.

We perform an extensive numerical study of avalanche behavior in a two-dimensional Lennard-Jones glass at T = 0, sheared at finite strain rates gamma[over]. From the finite size analysis of stress fluctuations and of transverse diffusion we show that flip-flip correlations remain relevant at all realistic strain rates. We predict that, in steady flow, the avalanche size scales as gamma[over];{-1/d}, with d the space dimension.