Publications by authors named "Carole E Rolph"
Article Synopsis
- The investigation of yeast neutral lipid accumulation is crucial for biotechnological applications and studying lipid metabolism disorders in humans.
- The Nile red method has been a popular technique used for assessing lipid levels in yeast cells but requires further validation, especially concerning genetically modified organisms.
- A modified Nile red assay has been successfully utilized on the fission yeast Schizosaccharomyces pombe, allowing for efficient screening of lipid phenotypes in various strains, ultimately streamlining lipid analysis in both research and industry settings.
Xerostomia (oral dryness sensation) is due to dryness of the oral cavity and it is more prevalent in the elderly. This study investigated the effect of ageing on parotid gland structure and function of control (2-6 months) and aged (12, 16-18 and 22-24 months) rats employing light microscopic, colorimetric, gas chromatographic and microspectrofluorimetric methods to investigate the morphological changes of the parotid glands, amylase release, endogenous lipid distribution and cytosolic free calcium levels, respectively. When compared to controls, age-related changes were apparent in glands obtained from rats aged 16-18 and 22-24 months, which included reduced acinar cell distribution, enlarged parotid ducts with fatty and connective tissue and mast cell infiltrations.
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