Mumps Outbreak in a Marshallese Community - Denver Metropolitan Area, Colorado, 2016-2017.

In January 2017, the Colorado Department of Public Health and Environment (CDPHE) identified four epidemiologically linked cases of mumps among persons from a Marshallese community who were members of the same church in the Denver metropolitan area. During 2016-2017, sizable outbreaks of mumps reported in Arkansas, Hawaii, and Washington also affected the Marshallese population (1). CDPHE, the Tri-County Health Department (TCHD), and Denver Public Health collaborated to conduct an outbreak investigation during January-March 2017 using active and passive surveillance that identified 17 confirmed and 30 probable cases.

Public Health Economic Burden Associated with Two Single Measles Case Investigations - Colorado, 2016-2017.

During July 2016-January 2017, two unrelated measles cases were identified in the Denver, Colorado area after patients traveled to countries with endemic measles transmission. Each case resulted in multiple exposures at health care facilities and public venues, and activated an immediate and complex response by local and state public health agencies, with activities led by the Tri-County Health Department (TCHD), which serves Adams, Arapahoe, and Douglas counties. To track the economic burden associated with investigating and responding to single measles cases, personnel hours and supply costs incurred during each investigation were tracked prospectively.

Genomic imprinting defect in Zfp57 mutant iPS cell lines.

ZFP57 maintains genomic imprinting in mouse embryos and ES cells. To test its roles during iPS reprogramming,we derived iPS clones by utilizing retroviral infection to express reprogramming factors in mouse MEF cells. After analyzing four imprinted regions, we found that parentally derived DNA methylation imprint was largely maintained in the iPS clones with Zfp57 but missing in those without maternal or zygotic Zfp57.

A critical analysis of online nursing education: balancing optimistic and cautionary perspectives.

The landscape of nursing education has been transformed by increasing student demand for online programs coupled with strong institutional directives to deliver nursing courses through distributed learning. The authors present a qualitative research design informed by philosophical hermeneutics in which 30 undergraduate and graduate nursing students discuss their experiences of the influence of peer dynamics on online learning. The findings include issues related to time, demands of online participation, experiences of conflict, and the development of skills in the online environment.

Contemplating the fit and utility of nursing theory and nursing scholarship informed by the social sciences and humanities.

In this article, authors contend that a lack of familiarity with philosophical thinking undermines the ability of students and subsequently practicing nurses to theorize for themselves. Engagement with philosophical ideas propels nurses well beyond the unthinking "application" of extant theory, to theorizing, that is, using theoretical formulations to engage with the significant phenomena we encounter in the world of human health. The authors present a framework to guide philosophical interrogation of knowledge, with a focus on the utility of both disciplinary knowledge and knowledge from the social sciences and humanities.

Mechanisms of triglyceride accumulation in activated macrophages.

LPS treatment of macrophages induces TG accumulation, which is accentuated by TG-rich lipoproteins or FFA. We defined pathways altered during macrophage activation that contribute to TG accumulation. Glucose uptake increased with activation, accompanied by increased GLUT1.

Unveiling new dimensions: a hermeneutic exploration of perinatal mood disorder and infant feeding.

In this hermeneutic study, six women from the local Perinatal Mental Health Program were interviewed about their experiences with infant feeding. None of the women in the study were breastfeeding their infants. The research question centered on their experience of formula feeding with a view to gaining better insight about the issues that women face when feeding practices do not conform to best-practice (i.

The limitations of partial citizenship: health care institutions underpinned with heteronormative ideals.

Tensions between apparent rights of lesbian, gay, bisexual, transgender, or queer/questioning people and the continued prevalence of heteronormative health care practice emerged in research with older women who self-identified as lesbian. Having already faced considerable exclusionary institutional practices in their lives, these women expressed anticipatory dread of the erasure of their lives in residential or long-term care. Drawing on the framework of sexual citizenship, this article critiques the disjuncture between the legal reality and the lived reality of LGBTQ people and suggests that social or political rights of full citizenship remain tenuous or absent in residential care settings.

Zinc finger protein ZFP57 requires its co-factor to recruit DNA methyltransferases and maintains DNA methylation imprint in embryonic stem cells via its transcriptional repression domain.

Previously, we discovered that ZFP57 is a maternal-zygotic effect gene, and it maintains DNA methylation genomic imprint at multiple imprinted regions in mouse embryos. Despite these findings, it remains elusive how DNA methyltransferases are targeted to the imprinting control regions to initiate and maintain DNA methylation imprint. To gain insights into these essential processes in genomic imprinting, we examined how ZFP57 maintains genomic DNA methylation imprint in mouse embryonic stem (ES) cells.

Dialectic of the university: a critique of instrumental reason in graduate nursing education.

Our analysis in this paper unfolds on two levels: a critique of the 'realities' of graduate nursing education and an argument to sustain its 'ideals'. We open for discussion an aspect of graduate nursing education dominated by instrumental reason, namely the research industry, using an internal critique approach developed by Max Horkheimer and Theodor Adorno of the Early Frankfurt School. As we explain, internal critique arises out of, and relies on, the mismatch between goals, or 'ideals', and existing realities.

Bringing ourselves into view: disclosure as epistemological and ontological production of a lesbian subject.

Using Gadamerian hermeneutics as a methodology and feminist philosophical thought as an analytical framework, this study explores understandings of experiences of disclosure of sexual orientation for older 'lesbian' women. The study draws on an interpretative inquiry in which participants theoretically align with sexual identity categories with both an ontological and an epistemological purpose and later move away from or even disassociate from the 'category' (of lesbian). Reflecting on these interpretations, that of the epistemological and the ontological production of a subject, we ask: What does it mean then to say I am a lesbian? This is where a tension exists: how is it that in spite of the incongruencies of what constitutes a lesbian, and the apparent ambiguity of people to name themselves, we continue to act (as health care providers and researchers) as if the category itself is meaningful and stable? A feminist lens provides an inroad to consider sexuality as practices, rather than as fixed identity; practices that are constituted within the discursive, social and material realities of a life as well as within political and ideological systems in which one resides.

Regulation of the Epstein-Barr virus Zp promoter in B lymphocytes during reactivation from latency.

Ten novel mutations were introduced into the Zp promoter to test the role of sequences outside the established transcription factor-binding sites in Epstein-Barr virus (EBV) reactivation. Most of these had only small effects, but mutations in the ZID site were shown to reduce Zp activity strongly at early times after induction by anti-immunoglobulin (anti-Ig). The binding of MEF2 transcription factor to ZID was characterized in detail and linked functionally to Zp promoter activity.

ADRP/ADFP and Mal1 expression are increased in macrophages treated with TLR agonists.

Activation of macrophages by TLR agonists enhances foam cell formation, but the underlying mechanisms are not understood. We examined the effects of TLR agonists on ADRP/ADFP, a protein associated with forming lipid droplets, and Mal1 a fatty acid-binding protein, in two mouse macrophage cell lines and human monocytes. Low doses of LPS, a TLR4 agonist increased both mRNA and protein levels of ADRP/ADFP and Mal1 in RAW 264.

Dilemmas, tetralemmas, reimagining the electronic health record.

With the transition from paper-based to computer-based records, nursing practice shifts to computerized documentation of care in the electronic health record (EHR). Viewed not only as an electronic document, but as an instrument of modern economic and technological ideology that serves organizational goals of cost-efficiency, the EHR can be perceived as creating a dilemma for a patient-centered nursing practice. Viewing the EHR as relying solely on the use of standardized languages begets a number of questions and furthers the dilemma for nurses.

Lesbian disclosure: disrupting the taken for granted.

The purpose of this interpretive inquiry was to generate understandings about the experience of lesbian disclosure. The inquiry relied on Gadamerian hermeneutic and feminist philosophical thought and was situated in women's health. In a feminist understanding of women's health, experiences of health are inseparable from the everyday experiences of an embodied life and are constituted within each woman's social, material, and discursive realities.

Interaction of Epstein-Barr virus BZLF1 C-terminal tail structure and core zipper is required for DNA replication but not for promoter transactivation.

The Epstein-Barr virus (EBV) protein BZLF1 contains a bZIP DNA binding domain in which C-terminal tail residues fold back against a zipper region that forms a coiled coil and mediates dimerization. Point mutagenesis in the zipper region reveals the importance of individual residues within the (208)SSENDRLR(215) sequence that is conserved in C/EBP for transactivation and EBV DNA replication. The restoration of BZLF1 DNA replication activity by the complementation of two deleterious mutations (S208E and D236K) indicates that the interaction of the C-terminal tail and the core zipper is required for DNA replication, identifying a functional role for this structural feature unique to BZLF1.

Unpacking disclosure: interrupting unquestioned practices.

Disclosure, the social practice of sharing the details, the "truths" of one's life, is embedded in our social world, and in the assumptions that drive the provision of mental health and psychotherapeutic intervention. In this paper the author interrogates the ways in which categories of sexuality and disclosure itself is historically derived and asks: How would care be different if we consider the constructed categories of sexual orientation as an exemplar of an unstable truth? In addition, the author suggests approaches to practice that unseat the valorization of disclosure.

Lesbian disclosure: disrupting the taken for granted.

The purpose of this interpretive inquiry was to generate understandings about the experience of lesbian disclosure. The inquiry relied on Gadamerian hermeneutic and feminist philosophical thought and was situated in women's health. In a feminist understanding of women's health, experiences of health are inseparable from the everyday experiences of an embodied life and are constituted within each woman's social, material, and discursive realities.

Adipocyte fatty acid-binding protein expression and lipid accumulation are increased during activation of murine macrophages by toll-like receptor agonists.

Objective: Toll-like receptors (TLRs) recognize pathogens and mediate signaling pathways important for host defense. Recent studies implicate TLR polymorphisms in atherosclerosis risk in humans. Adipocyte fatty acid-binding protein (aP2) is present in macrophages and has an important role in atherosclerotic plaque development.

The view from somewhere: locating lesbian experience in women's health.

In some circles of nursing practice and research, "women's health" is seen to stand for "women's reproductive and heterosexual health." In this article we offer a view of women's health that makes central the contexualized reality of the material, social, and discursive experiences of women's lives. We suggest that the barriers to adopting broader conceptualizations of health are grounded in pervasive ideologies that privilege biology and a traditional model of health over nonmedical determinants of health.