Publications by authors named "Carol Kreader"
The serum or plasma microRNA (miRNA) molecules have been suggested as diagnostic and prognostic biomarkers, in various pathological conditions. However, these molecules are also found in different serum fractions, such as exosomes and Argonaute (Ago) protein complexes. Ago1 is the predominant Ago protein expressed in heart tissue.
View Article and Find Full Text PDFArtificial transcription factors are powerful tools for regulating gene expression. Here we report results with engineered zinc-finger transcription factors (ZF-TFs) targeting four protein-coding genes, OCT4, SOX2, KLF4 and c-MYC, and one noncoding ribonucleic acid (RNA) gene, the microRNA (miRNA) miR302/367 cluster. We designed over 300 ZF-TFs whose targets lie within 1 kb of the transcriptional start sites (TSSs), screened them for increased messenger RNA or miRNA levels in transfected cells, and identified potent ZF-TF activators for each gene.
View Article and Find Full Text PDFArticle Synopsis
- The Target ID Library helps researchers identify microRNA (miRNA) targets by using a plasmid-based cDNA library that includes sequences from various human tissues and cell lines.
- It involves a multi-step selection process where stable transformants first grow, followed by the introduction of a specific miRNA, and finally selecting for cDNAs that contain the miRNA's target.
- The library covers a large portion of the human transcriptome, with over 79% of unique genes represented, and has been sequenced to confirm its diversity and effectiveness in identifying gene targets.