Enhancing Transcriptional Reprogramming of Mesenchymal Glioblastoma with Grainyhead-like 2 and HDAC Inhibitors Leads to Apoptosis and Cell-Cycle Dysregulation.

Glioblastoma (GBM) tumor cells exhibit mesenchymal properties which are thought to play significant roles in therapeutic resistance and tumor recurrence. An important question is whether impairment of the mesenchymal state of GBM can sensitize these tumors to therapeutic intervention. HDAC inhibitors (HDACi) are being tested in GBM for their ability promote mesenchymal-to-epithelial transcriptional (MET) reprogramming, and for their cancer-specific ability to dysregulate the cell cycle and induce apoptosis.

Affinity Purification of NF1 Protein-Protein Interactors Identifies Keratins and Neurofibromin Itself as Binding Partners.

Neurofibromatosis Type 1 (NF1) is caused by pathogenic variants in the gene encoding neurofibromin. Definition of NF1 protein-protein interactions (PPIs) has been difficult and lacks replication, making it challenging to define binding partners that modulate its function. We created a novel tandem affinity purification (TAP) tag cloned in frame to the 3' end of the full-length murine cDNA ().

Multi-Omics Profiling for NF1 Target Discovery in Neurofibromin (NF1) Deficient Cells.

Loss of NF1 is an oncogenic driver. In efforts to define pathways responsible for the development of neurofibromas and other cancers, transcriptomic and proteomic changes are evaluated in a non-malignant NF1 null cell line. NF1 null HEK293 cells were created using CRISPR/Cas9 technology and they are compared to parental cells that express neurofibromin.

Advocacy Recruiting for Huntington's Disease Clinical Trials.

Recent clinical trials for Huntington's disease (HD) have been slowed by the inability to complete enrollment in a timely manner. We report a successful advocacy-based recruiting approach at Evergreen Neuroscience Institute, a new Huntington Study Group (HSG) investigative site that lacked an HD patient base. By partnering with community advocates and utilizing web-based advocacy group alerts, Evergreen ranked third of 27 North American sites conducting the Study of ACR16 for the Treatment of Huntington's disease (HART) for number of participants, and first for rate of recruitment -- all while decreasing the time and financial resources needed for site-based recruiting.

Pathology of inhalation anthrax in cynomolgus monkeys (Macaca fascicularis).

Anthrax is considered a serious biowarfare and bioterrorism threat because of its high lethality, especially by the inhalation route. Rhesus macaques (Macaca mulatta) are the most commonly used nonhuman primate model of human inhalation anthrax exposure. The nonavailability of rhesus macaques necessitated development of an alternate model for vaccine testing and immunologic studies.

Correlation of atherectomy specimen histology with coronary arteriographic lesion morphologic appearance in patients with stable and unstable angina.

Complex lesions on coronary arteriography are seen commonly in patients with unstable angina and have been considered to be evidence of ruptured plaque with or without thrombus. To investigate the cause of complex lesions the histologic findings in atherectomy-derived specimens in 111 patients were correlated with lesion morphologic appearance on coronary arteriography. Among 91 patients with complex lesions, 81.

Effect of adrenaline on plasma concentrations of bupivacaine following intra-articular injection of bupivacaine for knee arthroscopy.

We administered 0.5% bupivacaine 30 ml either with or without adrenaline 5 micrograms ml-1 randomly to 16 healthy outpatients, to determine the efficacy of local and intra-articular local anaesthesia for knee arthroscopy and whether or not adrenaline should be added to intra-articular bupivacaine. Bupivacaine concentrations were measured in plasma obtained 15, 30, 45 and 60 min after intra-articular injection.

Effects of parachlorophenylalanine, quipazine and cyproheptadine on growth hormone and adrenocorticotropin secretion in steers.

Adrenergic and perhaps dopaminergic neurons provide inhibitory regulation of growth hormone (GH) secretion in ruminants. This suggests that either serotonergic or other neurons regulate the stimulatory release of GH. The nature of neurotransmitter control of adrenocorticotropin (ACTH) secretion in ruminants has not been determined.

Effect of propionate infusion on plasma glucagon, insulin and growth hormone concentrations in lactating dairy cows.

Altered concentrations of metabolic hormones have been suggested as important mediators of energy partitioning during early lactation. This study was initiated to determine the effects of propionate (1.0 mmol/kg body weight) infusion on plasma concentrations of glucagon, insulin, growth hormone, propionate and glucose at 14 days ante-partum (AP) and days 5 and 30 postpartum (PP).

Cancer incidence and mortality in Newark, N. J. 1970-1974: a national comparison.

New Jersey has acquired the invidious label "Cancer Alley U. S. A.

Comparison of the bacterial clearing effects of different biologic dressings on granulating wounds following thermal injury.

In 16 patients with thermal injury, no difference in bacterial clearing was noted when wounds were dressed with cadaver homograft skin, porcine skin, either mesh or sheet, and with amnion. Because of cost and ease we prefer fresh porcine skin for second- and third-degree burns.

Biological dressings and evaporative water loss from burn wounds.

Increased evaporative water loss following thermal injury sometimes results in electrolyte abnormalities, negative nitrogen balance, and hypothermia. Because different biological dressings have been claimed effective in diminishing evaporative loss, a prospective study was designed to compare them. Cadaver allograft, porcine xenograft (sheet and meshed), and amnion were placed on 28 granulating wounds for twenty-four hours.

Hypercalcemic crisis associated with the hypereosinophilic syndrome.

A patient with the idiopathic hyperosinophilic syndrome had severe, life-threatening hypercalcemia, a previously unreported complication of this unusual disease. Postmortem examination revelaed multiorgan eosinophilic infiltration and diffuse metastatic calcifications, but it did not disclose a definitive cause for the persistent hypercalcemia. It is concluded that the marked proliferation of eosinophils was the probable etiologic factor.