Publications by authors named "Carminda Sandra Brito Salmito Vanderley"
The cooling of shrimp spermatophores for assisted insemination can enable the transfer of gametes between reproduction laboratories. This study aimed to assess three extenders for cooling spermatophores for assisted insemination. Spermatophores were chilled at 15 °C for 24 or 48 hours using powdered coconut water ACP® (PCW), mineral oil (MO), and sterilized seawater (SSW) as extenders.
View Article and Find Full Text PDFEffects of taurine, cysteine and melatonin as antioxidant supplements to the freezing medium of Prochilodus brevis sperm.
Cryobiology
March 2024
Cryopreservation consist of a set of methods to preserve cells and tissues by drastically reducing the temperature. Among some undesired effects, cryopreservation might generate reactive oxygen species that lead to an increase of oxidative stress, causing damage to cells. This study aimed to test taurine, cysteine, and melatonin on the freezing of Prochilodus brevis sperm and assess its effects on post-thawed sperm quality.
View Article and Find Full Text PDFArticle Synopsis
- The study investigated four non-permeable cryoprotectants (egg yolk, soy lecithin, sucrose, and lactose) for their effects on sperm cryopreservation.
- The inclusion of egg yolk significantly improved sperm membrane integrity and motility compared to the control group.
- The findings suggest that a combination of 5% egg yolk with glucose and dimethyl sulfoxide is the most effective approach for preserving sperm quality in this species.
Determination of the ideal volume of activating solution and the optimal ratio of spermatozoa per oocyte for fertilization.
Zygote
April 2020
Article Synopsis
- Prochilodus brevis is a fish species with declining populations, prompting research on improving its reproduction in captivity.
- The study aimed to identify the optimal sperm-to-oocyte ratio and the best volume of activating solution for fertilization.
- Results showed that using approximately 928,410 sperm per oocyte and 75.64 mL of activating solution per 2 mL of oocytes maximized fertilization rates, achieving a peak of 51.69%.