Publications by authors named "Carmina Espiritu"
Positive and negative controls with known expression of target proteins are essential for the development of immunohistochemistry (IHC) assays. While tissue controls are beneficial for well-characterized proteins with defined tissue and cellular expression patterns, they are less suitable for the initial development of IHC assays for novel, poorly characterized, or ubiquitously expressed proteins. Alternatively, due to their standardized nature, cell pellets, including cancer cell lines with defined protein or transcript expression levels (e.
View Article and Find Full Text PDFImmunohistochemistry (IHC) assays provide valuable insights into protein expression patterns, the reliable interpretation of which requires well-characterized positive and negative control samples. Because appropriate tissue or cell line controls are not always available, a simple method to create synthetic IHC controls may be beneficial. Such a method is described here.
View Article and Find Full Text PDFWith the advent of checkpoint inhibitors, there is increasing need to study the dynamics of CD8+ T-cells in the tumor microenviroment. In this article, we describe a semi-automated method to quantify and interrogate spatial relationships between T-cells and collagenous stroma in human and mouse tissue samples. The assay combines CD8 immunohistochemistry with modified Masson's trichrome.
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- * The database includes RNA sequencing data from 129 donors, along with genome-wide expression data and single-nucleus RNA sequencing from over 100,000 cells, which helps to identify gene expression patterns related to AMD.
- * Researchers found 15 potential causal genes for AMD, like TSPAN10 and TRPM1, showing how this database can be useful for discovering new genetic pathways and therapeutic options for eye diseases.
Aim: Recently, the Oncotype DX recurrence score, which measures a gene expression signature including markers of tumour proliferation, was validated as a prognostic signature in colorectal cancer. This study aimed to evaluate whether the Ki67 proliferation index can provide similar prognostic and predictive information.
Methods: Tissue microarrays were constructed from triplicate cores of colorectal cancer.