Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target.

Drugs that target histone deacetylase (HDAC) entered the pharmacopoeia in the 2000s. However, some enigmatic phenotypes suggest off-target engagement. Here, we developed a quantitative chemical proteomics assay using immobilized HDAC inhibitors and mass spectrometry that we deployed to establish the target landscape of 53 drugs.

Role of wide-field autofluorescence imaging and scanning laser ophthalmoscopy in differentiation of choroidal pigmented lesions.

Aim: To evaluate the diagnostic properties of wide-field fundus autofluorescence (FAF) scanning laser ophthalmoscope (SLO) imaging for differentiating choroidal pigmented lesions.

Methods: A consecutive series of 139 patients were included, 101 had established choroidal melanoma with 13 untreated lesions and 98 treated with radiotherapy. Thirty-eight had choroidal nevi.

Systematic analysis of wide-field fundus autofluorescence (FAF) imaging in posterior uveitis.

Purpose: To evaluate wide-field fundus autofluorescence (FAF) in patients with uveitis with retinal or chorioretinal involvement.

Materials And Methods: We included 78 study eyes in this prospective study. Best-corrected visual acuity, a full clinical examination, wide-field green-light FAF and composite color, green and red laser separation fundus imaging with Optomap SLO were performed.

Peripheral fundus autofluorescence is increased in age-related macular degeneration.

Purpose: To evaluate peripheral fundus autofluorescence (FAF) in patients with AMD.

Methods: A consecutive series of 71 normal eyes, 71 eyes with neovascular AMD having received anti-VEGF treatment, and 43 eyes with untreated AMD were investigated. In all subjects, wide-field FAF imaging was performed, applying a wide-field scanning laser ophthalmoscope.

Wide-field fundus autofluorescence corresponds to visual fields in chorioretinitis patients.

Background And Objectives: Detection of peripheral fundus autofluorescence (FAF) using conventional scanning laser ophthalmoscopes (SLOs) is difficult and requires pupil dilation. Here we evaluated the diagnostic properties of wide-field FAF detected by a two-laser wavelength wide-field SLO in uveitis patients.

Study Design/materials And Methods: Observational case series of four patients suffering from different types of posterior uveitis/chorioretinitis.

Choroidal pigmented lesions imaged by ultra-wide-field scanning laser ophthalmoscopy with two laser wavelengths (Optomap).

Purpose: Clinical differentiation of choroidal pigmented lesions is sometimes difficult. Choroidal melanoma is the most prevalent primary neoplasia among malignant ocular tumors, and metastasis often occurs before the primary tumor is diagnosed. Therefore, early detection is essential.