Publications by authors named "Carmen Martin-Hernandez"
Five methods using aqueous/organic solvents for the separation of proteins from oils were compared. The extraction with acetone-hexane followed by amino acid analysis was found to be the most suitable method for isolation and quantification of proteins from oils. The detection limit of the method was 0.
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- A comparison of methods for extracting and quantifying proteins from lecithins highlighted hexane-2-propanol-water extraction as the most effective, achieving detection limits of 15 mg/kg and quantification limits of 50 mg/kg.
- Protein recovery rates were high, ranging from 101% to 123%, and varied across different lecithin sources, with standard soy lecithins containing up to 1338 mg/kg of protein.
- Analysis using mass spectrometry identified key proteins in soy and sunflower lecithins, mainly from the 11S globulin fraction, while soy lecithin also contained the allergenic protein P34 from the 7S globulin fraction.