Neurogenesis and brain injury: managing a renewable resource for repair.

The brain shows limited ability to repair itself, but neurogenesis in certain areas of the adult brain suggests that neural stem cells may be used for structural brain repair. It will be necessary to understand how neurogenesis in the adult brain is regulated to develop strategies that harness neural stem cells for therapeutic use.

Background and tandem-pore potassium channels in magnocellular neurosecretory cells of the rat supraoptic nucleus.

Magnocellular neurosecretory cells (MNCs) were isolated from the supraoptic nucleus of rat hypothalamus, and properties of K(+) channels that may regulate the resting membrane potential and the excitability of MNCs were studied. MNCs showed large transient outward currents, typical of vasopressin- and oxytocin-releasing neurons. K(+) channels in MNCs were identified by recording K(+) channels that were open at rest in cell-attached and inside-out patches in symmetrical 150 mM KCl.

Characterization of four types of background potassium channels in rat cerebellar granule neurons.

Cerebellar granule neurons express a standing outward (background) K+ current (I(K,SO)) that regulates the resting membrane potential and cell excitability. As several tandem-pore (2P) K+ channel mRNAs are highly expressed in cerebellar granule cells, we studied whether, and which, 2P K+ channels contribute to I(K,SO). I(K,SO) was highly sensitive to changes in extracellular pH and was partially inhibited by acetylcholine, as reported previously.

Functional expression of TREK-2 K+ channel in cultured rat brain astrocytes.

Background K+ channels whose subunit contains four transmembrane segments and two pore-forming domains (4TM/2P) have been cloned recently. We studied whether 4TM/2P K+ channels are functionally expressed in astrocytes that are known to have a large background (resting) K+ conductance and a large resting membrane potential. Reverse transcriptase-PCR analysis showed that, among five 4TM/2P K+ channels examined, TASK-1, TASK-3 and TREK-2 mRNAs were expressed in cultured astrocytes from rat cortex.