Protocol for extracting live blastoderm cells from embryos of annual killifish.

The implementation of in vitro approaches using undifferentiated embryonic cells from annual killifish to complement existing in vivo developmental studies has been hindered by a lack of efficient isolation techniques. Here, we present a protocol to isolate annual killifish blastoderm cells, at the epiboly and early dispersion phase, from embryos. We describe steps for hair removal, embryo cleaning, dechorionation, and cell purification.

Expression of in the Olfactory System of Embryonic Zebrafish ().

The Reprimo () family is composed of highly conserved single-exon genes. The expression pattern of this gene family has been recently described during zebrafish () embryogenesis, and primarily locates in the nervous system. Its most characterized member, , which duplicated to give rise and in the fish lineage, is known to act as a tumor-suppressor gene in mammalian models.

Directional asymmetry in the volume of the human habenula.

Brain asymmetry is a conserved feature in vertebrates. The dorsal diencephalic habenular complex shows conspicuous structural and functional asymmetries in a wide range of species, yet it is unclear if this condition is also present in humans. Addressing this possibility becomes relevant in light of recent findings presenting the habenula as a novel target for therapeutic intervention of affective disorders through deep brain stimulation.