Analysis of Polyhydroxyalkanoates Granules in by Double-Fluorescence Staining with Nile Red and SYBR Green by Confocal Fluorescence Microscopy.

is a haloarchaeon of high interest in biotechnology because it produces and mobilizes intracellular polyhydroxyalkanoate (PHA) granules during growth under stress conditions (limitation of phosphorous in the culture media), among other interesting metabolites (enzymes, carotenoids, etc.). The capability of PHA production by microbes can be monitored with the use of staining-based methods.