Cytoprotective and anti-apoptotic action of HSP70 stress protein in exposed to residual dilutions of insecticides with fipronil and ethiprole.

The objective of this research was to investigate the potential damage caused by the residual concentrations of the insecticides Regent WS 800 and Curbix SC 200, containing fipronil and ethiprole, respectively as active ingredients, on the liver of . The analyses of HSP70 shock protein labelling and cell death process by TUNEL method were performed in order to measure the effects of the exposure of cell repair system of fish to both insecticides. Statistical analyses showed no significant molecular damage to the hepatic tissue of animals.

Toxicity evaluation of leached of sugarcane vinasse: Histopathology and immunostaining of cellular stress protein.

Sugarcane vinasse is a residue generated at a rate fifteen times greater than the ethanol production. Because of its high organic and micronutrient content, this residue is used as a fertilizer on sugarcane crops. However, when used in large quantities, vinasse can saturate the soil and contaminate nearby water resources by percolation and leaching.

Toxicity evaluation of vinasse and biosolid samples in diplopod midgut: heat shock protein in situ localization.

Large amounts of residues generated by agricultural, urban and industrial activities are dumped daily on the soil. This practice deserves special attention because it causes serious environmental problems. This study evaluated the toxic potential of the sugarcane vinasse, a by-product of the sugar-alcohol industry, and the biosolid, a residue produced by wastewater treatment plants, both widely used as fertilizers.

Evaluation of herbicides action on plant bioindicators by genetic biomarkers: a review.

The use of pesticides has increased worldwide, owing to the demand for products of good quality and to satisfy a growing population. Herbicides represent almost half of the total amount of pesticides used. Although important to the reduction of costs and an increase of productivity, their indiscriminate use, as well as that of the other pesticides, is a global environmental problem, since they affect the living organisms.

Genotoxicity evaluation of two metallic-insecticides using Allium cepa and Tradescantia pallida: A new alternative against leaf-cutting ants.

In order to combat leaf-cutting ants, the pesticide sulfluramid used to be the most widely utilized active ingredient. However, its use was banned in 2009 by the Stockholm Convention, although some countries were allowed to continue using it. As an effective alternative to its replacement, researchers developed a metallic-insecticide system, which is a natural product linked to metal complexes.

Histopathological changes in the perivisceral fat body of Rhinocricus padbergi (Diplopoda, Spirobolida) triggered by biosolids.

Human activities generate a great amount of sewage daily, which is dumped into the sewer system. After sewage-treatment processes, sewage sludge is generated. Such byproduct can be treated by different methods; the result of treatment is a stabilized compost of reduced pathogenicity that has a similar inorganic chemical composition to the raw sewage sludge.

Sugar cane vinasse in water bodies: impact assessed by liver histopathology in tilapia.

Aquatic ecosystems are the main receptors of toxic substances from human activities. With the increase in sugar cane production, vinasse - the main residue of ethanol production - is a potential contaminant of water resources, due to its high organic matter content. This study was aimed at evaluating the toxicity of vinasse by examining the liver of the fish Oreochromis niloticus exposed to different dilutions of sugar cane vinasse (1%, 2%, 5%, 5% and 10%) in laboratory bioassays.

The use of diplopods in soil ecotoxicology - a review.

Diplopods play an important role in the dynamics of terrestrial ecosystems, actively participating in the decomposition of organic matter and soil aeration. They have gained increased attention from ecotoxicology research because they are continuously exposed to soil contaminants and biological effects of chemical stressors can be measurable at various levels of biological organization. This paper is the first review on the use of diplopods as soil bioindicators and compiles the effects of the different toxic chemical agents on these animals.

Sugarcane vinasse: environmental implications of its use.

The inadequate and indiscriminate disposal of sugarcane vinasse in soils and water bodies has received much attention since decades ago, due to environmental problems associated to this practice. Vinasse is the final by-product of the biomass distillation, mainly for the production of ethanol, from sugar crops (beet and sugarcane), starch crops (corn, wheat, rice, and cassava), or cellulosic material (harvesting crop residues, sugarcane bagasse, and wood). Because of the large quantities of vinasse produced, alternative treatments and uses have been developed, such as recycling of vinasse in fermentation, fertirrigation, concentration by evaporation, and yeast and energy production.

Morphological and histochemical characterization of gill filaments of the Brazilian endemic bivalve Diplodon expansus (Küster, 1856) (Mollusca, Bivalvia, Hyriidae).

This study presents the morphological description and histochemical characterization of gill filaments of the Brazilian endemic bivalve Diplodon expansus, aiming to broaden the morphological knowledge of this species and establish the structure of the gills that will serve as control in histopathological studies applied to biomonitoring. The gill filaments are divided into three zones: frontal, intermediate, and abfrontal. In the center of the filament, haemocytes circulate through the haemolymph vessel, which is internally lined by endothelium.

Toxicity of trifluralin herbicide in a representative of the edaphic fauna: histopathology of the midgut of Rhinocricus padbergi (Diplopoda).

The aim of this study was to use the diplopod Rhinocricus padbergi as test organism to verify the possible toxic effects of the herbicide trifluralin in a representative of the edaphic fauna. Histological and histochemical techniques were applied in the midgut of the animals after exposure to different concentrations of trifluralin for 7 and 90 days (acute and subchronic exposure, respectively). The individuals from the control group did not present alterations to any of the exposures.

Clastogenicity of landfarming soil treated with sugar cane vinasse.

The addition of nutrients and/or soil bulking agents is used in bioremediation to increase microbial activity in contaminated soils. For this purpose, some studies have assessed the effectiveness of vinasse in the bioremediation of soils contaminated with petroleum waste. The present study was aimed at investigating the clastogenic/aneugenic potential of landfarming soil from a petroleum refinery before and after addition of sugar cane vinasse using the Allium cepa bioassay.

Alterations in the fat body cells of Rhinocricus padbergi (Diplopoda) resulting from exposure to substrate containing sewage sludge.

The final disposal of residues generated at sewage treatment plants (STPs) has become a major problem for cities, due to the increase in the amount of treated sewage. One of the alternatives for the residue, labeled "sewage sludge," is its reuse in agriculture and in degraded soil. However, not all pathogens and metals present in it are eliminated during treatment.

Sewage sludge toxicity in edaphic organism: analysis of midgut responses in the diplopod Rhinocricus padbergi.

Diplopods are agile saprophagous invertebrates with the ability to colonize several soil layers. They feed on waste and decaying organic matter, making the soil in which they are present better aerated and mineral-enriched. Because of their habits, diplopods have been suggested to be used in studies to analyze possible toxic effects of impacted soils in edafic fauna.

Surface morphology of Diplodon expansus (Küster, 1856; Mollusca, Bivalvia, Hyriidae) gill filaments after exposure to environmentally relevant concentrations of atrazine herbicide.

Brazilian endemic species Diplodon expansus (Küster, 1856) is found in freshwater bodies in the country's southeast, in large anthropogenic influence regions especially with an extensive agriculture emphasis. One of the main pesticides used in the species occurrence region is the atrazine herbicide, which has a great contamination potential in the aquatic environment. Therefore, several studies into its toxicity in aquatic systems have been developed.

Morphological biomarkers in the Rhinocricus padbergi midgut exposed to contaminated soil.

Morphological studies have been performed in invertebrates to elucidate cellular and/or tissue damage caused by environmental contamination. In present work, the exposure to industrial soil contaminated with polycyclic aromatic hydrocarbons and metals led to histological alterations in the midgut of Rhinocricus padbergi (Diplopoda). The following alterations were observed: increase of cytoplasmic granules in the fat body; increase in the number of regions of the epithelium in the process of renewal; increase in the number of hemocytes present among the cells of the fat body and degenerative changes in the midgut epithelium.

Hemocitical responses to environmental stress in invertebrates: a review.

Although invertebrates are recognized by the great facility to accumulate pollutants present in their environment and many of them are used as sentinel species in biomonitoring studies, little is known about the impact of toxicants on the immune system of these animals. In this regard, hemocytes play a fundamental role: these cells circulate freely through the hemolymph of invertebrates and act on the recognition of foreign material to the organism, mediating and effecting the cellular defense, such as phagocytosis, nodulation, and encapsulation. Different morphological types can be recognized but still there is controversy among the researchers about the exact classification of the hemocytes due to the diversity of techniques for the preservation and observation of these cells.

Acute and subchronic exposure of diplopods to substrate containing sewage mud: Tissular responses of the midgut.

Several discussions regarding the agricultural use of sewage mud have occurred; however, its use has been questioned due to the risks of soil contamination mainly by metals. Diplopods are saprophagous invertebrates, agile in colonizing several layers of soil. Due to the habit of this group, several researchers have proposed their use in ecotoxicological analyses.

The use of histological, histochemical and ultramorphological techniques to detect gill alterations in Oreochromis niloticus reared in treated polluted waters.

Fish gills are constituted by primary filaments and secondary lamellae, which are formed, basically, by three different cell types: pillar cells, respiratory cells and erythrocytes that circulate in the lamellae interior. This respiratory organ is very appreciated in environmental studies since it is constantly in contact with the water, accomplishing exchanges; besides this, it presents a high adaptation capacity. In this work, some fishes were reared in polluted waters treated by the flotation method (treated group) and compared with others that were reared in clean waters (control group).

Cytogenetics of four species of Spirostreptidae (Diplopoda, Spirostreptida).

Considering an estimated number of millipedes of approximately 80,000, cytogenetic studies on these animals are rare, as only a total of 70 species have their karyotypes described. The present study reports on the chromosomal number of four Brazilian diplopods of the family Spirostreptidae: Urostreptus atrobrunneus with 2n=24, XY; Gymnostreptus olivaceus 2n=12, XY and Alloporus araraquarensis and A. principes, 2n=18, XY.

Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauriès, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes.

Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this species is abundant in AT-rich sequences.