CENP-E Inhibition Induces Chromosomal Instability and Synergizes with Diverse Microtubule-Targeting Agents in Breast Cancer.

Drugs that perturb microtubules are commonly used to treat breast cancers of all subtypes in both early stage and metastatic disease, but they are effective in only approximately 50% of patients. High concentrations of microtubule-targeting agents can elicit mitotic arrest in cell culture models; however, recent evidence from primary and metastatic breast cancers has revealed that these agents only accumulate at intratumoral levels capable of inducing abnormal multipolar mitotic spindles, not mitotic arrest. Although the maintenance of multipolar spindles can generate cytotoxic rates of chromosomal instability (CIN), focusing of aberrant multipolar spindles into normal bipolar spindles can dramatically reduce CIN and confer resistance to microtubule poisons.

bacteraemia, cardiac implantable electronic device, extraction, and the risk of recurrent infection; a retrospective population-based cohort study.

Background: Patients with cardiac implantable electronic device (CIED) and bacteraemia (SAB) are at risk of having CIED infection, pocket infection or endocarditis. To avoid treatment failures, guidelines recommend that the CIED should be extracted in all cases of SAB butrecent studies indicate low extraction rates and low risk of relapse. The aim of the study was to describe a Swedish population-based cohort of patients with CIED and SAB, the rate of extraction, and treatment failure measured as recurrent SAB.

Diverse microtubule-targeted anticancer agents kill cells by inducing chromosome missegregation on multipolar spindles.

Microtubule-targeted agents are commonly used for cancer treatment, though many patients do not benefit. Microtubule-targeted drugs were assumed to elicit anticancer activity via mitotic arrest because they cause cell death following mitotic arrest in cell culture. However, we recently demonstrated that intratumoral paclitaxel concentrations are insufficient to induce mitotic arrest and rather induce chromosomal instability (CIN) via multipolar mitotic spindles.

Staphylococcus aureus bacteraemia, cardiac implantable electronic device, and the risk of endocarditis: a retrospective population-based cohort study.

Patients with cardiac implantable electronic device (CIED) and Staphylococcus aureus bacteraemia (SAB) are at risk of having infective endocarditis (IE). The objectives were to describe a Swedish population-based cohort of patients with CIED and SAB, to identify risk factors, and to construct a predictive score for IE. Patients over 18 years old in the Stockholm Region identified to have SAB in the Karolinska Laboratory database from January 2015 through December 2019 were matched to the Swedish Pacemaker and Implantable Cardioverter-Defibrillator ICD Registry to identify the study cohort.

Colonoscopy performance monitoring: do we need to adjust for case mix?

Introduction: Overall caecum intubation rate(oCIR) and overall polyp detection rate(oPDR) have been proposed as performance indicators, but varying complexity in case mix among endoscopists may potentially affect validity. The study aims to explore the effect of adjusting for case mix on individual endoscopist performance by calculating case mix-adjusted performance estimates (cmCIR and cmPDR) and comparing them to overall performance estimates (oCIR and oPDR). The study also provides an R program for case mix analysis.

Comparison of Race Time-Differences Between and Within Para and Able-Bodied Cross-Country Skiers.

Purpose: To compare differences in race time (i.e., the average percent difference in race time for each skier compared to the winner, RT) between female and male Para and able-bodied (AB) skiers, and to examine whether RT change across seasons.

Framework for In-Field Analyses of Performance and Sub-Technique Selection in Standing Para Cross-Country Skiers.

Our aims were to evaluate the feasibility of a framework based on micro-sensor technology for in-field analyses of performance and sub-technique selection in Para cross-country (XC) skiing by using it to compare these parameters between elite standing Para (two men; one woman) and able-bodied (AB) (three men; four women) XC skiers during a classical skiing race. The data from a global navigation satellite system and inertial measurement unit were integrated to compare time loss and selected sub-techniques as a function of speed. Compared to male/female AB skiers, male/female Para skiers displayed 19/14% slower average speed with the largest time loss (65 ± 36/35 ± 6 s/lap) found in uphill terrain.

Comparison of Physiological and Biomechanical Responses to Flat and Uphill Cross-Country Sit-Skiing in Able-Bodied Athletes.

Purpose: To compare peak work rate (WRpeak) and associated physiological and biomechanical performance-determining variables between flat and uphill cross-country (XC) sit-skiing.

Methods: Fifteen able-bodied male XC skiers completed 2 test sessions, each comprising four 4-minute submaximal stages, followed by an incremental test to exhaustion and a verification test in a sit-ski on a roller-ski treadmill. The test sessions were counterbalanced by the incline, being either 0.

Improving colonoscopy quality through individualised training programmes.

Introduction: In Denmark, quality-improvement initiatives aimed at providing a better colonoscopy service are few. The primary objective of this study was to improve colonoscopy quality at Aalborg University Hospital, Denmark, using structured training programmes. The secondary aim was to introduce a system for individual colonoscopist performance monitoring.

Colonoscopy adverse events: are we getting the full picture?

Introduction: Colonoscopy adverse events (AEs) are commonly underreported and standardised reporting is rarely used. We aimed to investigate AEs associated with colonoscopy in a real world setting, using the American Society of Gastrointestinal Endoscopy (ASGE) lexicon.

Methods: This retrospective cohort study of AEs related to outpatient colonoscopies performed in the North Denmark Region from 2015 to 2018 identified AEs from readmission within eight days or death within 30 days of colonoscopy.

Pole Length's Influence on Performance During Classic-Style Snow Skiing in Well-Trained Cross-Country Skiers.

Purpose: To investigate how self-selected pole length (PL) of ∼84% (PL84%) compared with ∼90% (PL90%) of body height influenced performance during a 700-m time trial with undulating terrain on snow.

Methods: Twenty-one cross-country skiers, 7 of whom were women, performed 4 trials at a maximal effort in a counterbalanced fashion with PL84% and PL90% separated by 20-minute breaks between trials. In trials I and II, only double poling was allowed, while in trials III and IV, skiers used self-selected classical subtechniques.

Effect of Blood Flow Restricted Resistance Exercise and Remote Ischemic Conditioning on Functional Capacity and Myocellular Adaptations in Patients With Heart Failure.

Background: Patients with congestive heart failure (CHF) have impaired functional capacity and inferior quality of life. The clinical manifestations are associated with structural and functional impairments in skeletal muscle, emphasizing a need for feasible rehabilitation strategies beyond optimal anticongestive medical treatment. We investigated whether low-load blood flow restricted resistance exercise (BFRRE) or remote ischemic conditioning (RIC) could improve functional capacity and quality of life in patients with CHF and stimulate skeletal muscle myofibrillar and mitochondrial adaptations.

The influence of pole lengths on O-cost, kinematics, and performance in double poling at high speeds before and after a training period with long poles.

Purpose: Previous studies have found an acute performance improvement with longer pole lengths in double poling (DP) at low-to-moderate speeds. We investigated the influence of pole lengths (PL) on O-cost, 3D kinematics, and performance in DP at moderate-to-high speeds before (Pre) and after (Post) eight training sessions with long poles on a rollerski treadmill.

Methods: Seven male and four female skiers completed tests with two different PLs (84 and 90% of body height).

Echocardiographic subtypes of heart failure in consecutive hospitalised patients with dyspnoea.

Unlabelled: Heart failure with preserved ejection fraction (HFpEF) involves half of hospitalised patients with heart failure (HF), but estimates vary due to unclear diagnostic criteria. We performed a prospective observational study of hospitalised patients admitted with dyspnoea. The aim was to apply contemporary guidelines to diagnose HF due to valvular disease (HFvhd), HF due to reduced ejection fraction (HFrEF), HF due to midrange EF (HFmrEF) and HFpEF in relation to presumed cardiac or non-cardiac dyspnoea.

Environmental transmission of Clostridioides difficile ribotype 027 at a long-term care facility; an outbreak investigation guided by whole genome sequencing.

Objective: This article describes a CDI outbreak in a long-term care (LTC) facility that used molecular typing techniques and whole-genome sequencing to identify widespread dissemination of the clonal strain in the environment which was successfully removed after terminal cleaning.

Setting: This study was conducted in a long-term care facility in Texas.

Methods: A recently hospitalized LTC patient was diagnosed with CDI followed shortly thereafter by 7 subsequent CDI cases.

Pole lengths influence O-cost during double poling in highly trained cross-country skiers.

Purpose: In elite cross-country skiing, double poling is used in different terrain. This study compared O-cost and kinematics during double poling with four different pole lengths [self-selected (SS), SS - 5 cm, SS + 5 cm, SS + 10 cm] at Low versus Moderate incline.

Methods: Thirteen highly trained male cross-country skiers (mean ± SD 23 ± 3 years; 182 ± 4 cm; 77 ± 6 kg) completed eight submaximal trials with roller skis on a treadmill at two conditions: "Low incline" (1.

Module based training improves and sustains surgical skills: a randomised controlled trial.

Purpose: Traditional surgical training is challenged by factors such as patient safety issues, economic considerations and lack of exposure to surgical procedures due to short working hours. A module-based clinical training model promotes rapidly acquired and persistent surgical skills.

Methods: A randomised controlled trial concerning supervised hernia repair in eight training hospitals in Denmark was performed.

Troponin T and N-terminal pro B-Type natriuretic peptide and presence of coronary artery disease.

Background: We tested the effects of exercise intensity, sampling intervals, degree of coronary artery stenosis, and demographic factors on circulating N-terminal pro B-Type natriuretic peptide (NT-pro-BNP) and cardiac Troponin T (cTnT) in subjects suspected of coronary artery disease (CAD).

Materials And Methods: A total of 242 subjects referred for diagnostic evaluation of possible CAD had blood samples obtained before, 5 min after, and again 20 h after a symptom-limited exercise test.

Results: Totally 40 subjects had CAD with ≥ 50% stenosis, 115 subjects had no stenosis and 87 subjects served as controls.

[One third of Danish doctors specializing in diagnostic radiology do not feel sufficiently equipped in terms of medical expertise].

An internet-based survey among Danish doctors specializing in diagnostic radiology revealed differences with respect to the length of internships at regional and university hospitals as well as the educational environment. The university hospitals were generally evaluated to have the best educational facilities except within practical skills. Therefore, it seems necessary to optimize the educational environment at regional hospitals, especially within medical expertise and scientific skills which are key elements regarding continuous development and quality assurance within radiology.

Is current surgical training efficient? A national survey.

Objectives: Evaluation of surgical training in Denmark is competency based with no requirement for a specific number of procedures. This may affect monitoring of surgical progress adversely and cause an underestimation of the time needed to acquire surgical competencies. We investigated the number of common surgical procedures performed by trainees.

High-sensitivity C-reactive protein and exercise-induced changes in subjects suspected of coronary artery disease.

Background: Inflammation plays a major role in the development of atherosclerosis. We wanted to investigate the effects of exercise on high-sensitivity (hs) C-reactive protein (CRP) in subjects who were suspected of having coronary artery disease (CAD).

Methods: Blood samples were obtained before, 5 minutes after, and 20 hours after an exercise test in 155 subjects who were suspected of CAD.

Reliable and valid assessment of Lichtenstein hernia repair skills.

Purpose: Lichtenstein hernia repair is a common surgical procedure and one of the first procedures performed by a surgical trainee. However, formal assessment tools developed for this procedure are few and sparsely validated. The aim of this study was to determine the reliability and validity of an assessment tool designed to measure surgical skills in Lichtenstein hernia repair.

Diagnostic value of exercise-induced changes in circulating high sensitive troponin T in stable chest pain patients.

Objective: We investigated the diagnostic value of exercise-induced increase in cardiac Troponin T (cTnT) in stable chest pain subjects.

Methods: CTnT was measured before and 20 h after an exercise test in 157 subjects suspected of coronary artery disease (CAD).

Results: CAD subjects (n = 41) had higher baseline cTnT levels compared to non-CAD subjects (n = 116), 6.

Prevalence and prognosis of heart failure with preserved ejection fraction and elevated N-terminal pro brain natriuretic peptide: a 10-year analysis from the Copenhagen Hospital Heart Failure Study.

Aims: The aim of this study was to assess the epidemiological features and prognosis of heart failure with preserved ejection fraction (HFPEF) and to compare these findings with those from patients with reduced ejection fraction. Furthermore the effects of N-terminal pro brain natriuretic peptide (NT-proBNP) requirement in the heart failure diagnosis were assessed by repeating the analyses in the subgroup of patients with elevated NT-proBNP.

Methods And Results: In 1844 patients admitted, a clinical diagnosis of heart failure was made in 433; amongst these 61% had HFPEF.