Publications by authors named "Carlota Quinones-Perez"
Despite the advances in reproductive technology, there is still a considerable number of low sperm quality cases in stallions. Recent studies in humans have detected several seminal microflora-spermatozoa associations behind some idiopathic infertility cases. However, no studies are available on horses, and there is limited information on the microflora present in stallion ejaculates.
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- High-throughput sequencing reveals that microbial communities in the male reproductive tract differ significantly between fertile and infertile males, with a focus primarily on humans, leaving a knowledge gap regarding domestic animals like stallions.
- This study aimed to characterize the seminal microbial composition of 12 healthy, fertile stallions using next-generation sequencing, detecting nine phyla, with Bacteroidetes, Firmicutes, and Actinobacteria being the most abundant.
- Notable findings include the identification of specific bacterial families such as Porphyromonadaceae and Prevotellaceae that dominate the seminal microbiome, while significant variability among individuals was observed, contrasting with findings from other species like humans.
Contagious equine metritis is receiving renewed attention due to the continuous detection of carriers in apparent agent-free farms. Interactions of with the seminal microflora may be the plausible cause behind these spontaneous changes of the carrier state. Accordingly, the aim of this study was to compare the differences in the seminal microbiome composition of one stallion in the contagious equine metritis carrier state and non-carrier state.
View Article and Find Full Text PDFDynamic assessment of sperm DNA fragmentation (SDF) has shown to give fuller understanding of stallion semen quality; however, there have been limited attempts to use this parameter to investigate seasonal changes in productive functions. The aims of this study were to: (a) establish a reliable mathematical model to describe the longevity of cooled-stored sperm DNA integrity; (b) to examine the effect of seasonal variations on SDF. Ejaculates were cooled to 5°C, and SDF was analysed after 0, 6 and 24 hr of storage.
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