A co-fermentation strategy with wood hydrolysate and crude glycerol to enhance the lipid accumulation in Rhodosporidium toruloides-1588.

Wood hydrolysate has been regarded as sustainable and renewable substrate to produce microbial lipids, a potential feedstock for the biodiesel industry. Moreover, the major by-product of biofuel industries is crude glycerol but its implementation as a carbon source is still constrained due to the presence of impurities resulting in low biomass production and low lipid titer. Thus, this study investigates the effect of different carbon ratios of hydrolysate and crude glycerol on R.

A critical insight into the development, regulation and future prospects of biofuels in Canada.

Renewable biofuel has a great potential in replacing the conventional transportation fuels as well as aiding the current issue of climate change and global warming. In the present scenario, tremendous initiatives have been implemented to encourage large-scale biofuel production and reduce greenhouse gas emissions. However, the information on the current biofuel status specifically in Canada and where it lacks in biofuel production, tax rebate and policies in comparison with other countries is limited.

Effect of creating a fed-batch like condition using carbon to nitrogen ratios on lipid accumulation in Rhodosporidium toruloides-1588.

Utilizing the undetoxified wood hydrolysate to accumulate maximum lipids in Rhodosporidium toruloides under optimum conditions has been regarded as a renewable and cost-effective strategy. The current investigation aims to identify the best carbon to nitrogen (C/N 20, 70, and 120) ratio for maximum lipid accumulation in R. toruloides-1588 using wood hydrolysate.