Comparison of CellRox green fluorescence upon thawing on and embryos cryopreserved by slow freezing or vitrification.

The aim of this study was to compare the levels of reactive oxygen species (ROS) in and embryos cryopreserved using either slow freezing or vitrification. Embryos were divided into four groups based on subspecies and freezing method: slow freezing (BiSF; = 8), vitrification (BiVT; = 10), slow freezing (BtSF; = 9), and vitrification (BtVT; = 6). After thawing, the embryos were incubated with CellRox Green and images were obtained using a confocal microscope.

Dual-Purpose Cattle Raised in Tropical Conditions: What Are Their Shortcomings in Sound Productive and Reproductive Function?

Dual-purpose husbandry might well be the most commonly employed cattle management system in tropical regions worldwide. The advantages of producing both meat and milk, although in reduced quantities, gives an edge to the farmer in coping with the volatile economic conditions that prevail in the region. Herein, we discuss the different methods of cattle management under tropical conditions based on the financial and social structure of this system.

Effects of Season on Donor and Recipient Cows and Calf Performance from Birth to Weaning in Embryo Transfer Programs in the Tropics.

The aim of this study was to assess the seasonal effect of an embryo transfer program in the tropics on the donor response, recipient reproductive performance and calf growth from birth to weaning. This study included five-year records from 145 donors, 1149 embryo transfers (ET) and 609 in calves. The effect of the season (dry or wet) was evaluated at the time of embryo flushing, embryo transfer and birth of the calves.

Effect of temperature - humidity index on the onset of post- partum ovarian activity and reproductive behavior in cows.

The effect of climatic factors on ovarian activity and reproductive behavior (RB) was evaluated in 46 cows kept under grazing conditions. Temperature-humidity index (THI) was used as an indicator of stress and divided in alert, damage and emergency levels. Fat thickness (FAT) was taken during the last trimester of gestation (LTG) to approximately 90d postpartum (PP).

The Use of Computer Records: A Tool to Increase Productivity in Dairy Herds.

The level of adoption of herd management information system veterinary automated management and production control program (VAMPP) Bovine and its impact on productivity of 912 specialized dairy systems with at least 5 years of information recorded was studied. Herds were classified as low ( = 389), medium ( = 343), or high ( = 180) adoption level on the basis of extent and consistency of record keeping for variables related to production, reproduction, and health. For each herd, within-year averages were obtained for six performance traits: age at first calving (AFC), days open (DO), daily milk yield (DMY), productive life (PL), incidence of clinical mastitis (MAST), and incidence of lameness (LAM).

Calf presence and estrous response, ovarian follicular activity and the pattern of luteinizing hormone in postpartum cows.

The main objective of the present experiment was to assess the effect of temporary weaning on the onset of estrus, ovarian follicular activity and secretion of luteinizing hormone in cows. Forty six mature cows were divided into three groups 1) calves were weaned for 72 h allowing auditory, olfactory and visual contact with their dams (VISUAL, n = 17), 2) calves without contact with their dams for 72 h (NC, n = 17) and 3) calves not weaned and in constant contact with their dams (CTRL, n = 12). Estrus was synchronized in all cows using CIDR for 9 days plus 2 mg of estradiol benzoate given at CIDR insertion.

Lipid droplets in clusters negatively affect Bos indicus embryos during cryopreservation.

Embryo transfer using cryopreserved B. indicus embryos results in low pregnancy rates. The low viability of B.