Publications by authors named "Carlos Quintans"
Objective: To report a birth of a healthy girl after long-term oocyte cryopreservation by slow cooling in sodium depleted medium.
Design: Clinical application.
Setting: University Affiliated, Private IVF center.
A 45-year-old woman received embryos from IVF by intracytoplasmic sperm injection (ICSI) with her own oocytes that were cryopreserved (slow freezing in a low-sodium medium) 11 years and 7 and a half months before, when she was 33 years old. From seven metaphase-II oocytes thawed, five survived, four were fertilized after ICSI and two cleaving embryos were transferred on day 3. A diamniotic dichorionic term pregnancy was achieved, ending with the delivery of two healthy girls.
View Article and Find Full Text PDFThe interest for South American camelids has increased in the last years. The aim of the present research was to compare the in vitro production of Lama glama embryos using two techniques: in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). For IVF technique, we compared the effect of adding or not, heparin, penicillamine and hypotaurine as sperm capacitating agents.
View Article and Find Full Text PDFArticle Synopsis
- The study investigated chromosomal abnormalities in embryos created through assisted reproductive technology using two techniques: fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH).
- It found that FISH was effective for analyzing individual cells in embryos, yielding results from all 10 tested, while CGH only provided usable data for 7 out of 30 embryos.
- Although CGH was more accurate overall for analyzing arrested embryos, FISH remains the preferred method for preimplantation genetic diagnosis due to its quicker results, allowing for timely decision-making during embryo culture.
Article Synopsis
- Oocyte cryopreservation, while promising for assisted reproductive technology, has had limited success, and this study reports on a new method using a low-sodium medium.
- In a retrospective analysis involving 12 patients aged 21-41, oocytes were cryopreserved in a modified salt solution and subsequently inseminated via ICSI.
- Results showed a median survival rate of 63%, a fertilization rate of 59%, and six clinical pregnancies, with two resulting in live births, marking a noteworthy advance in the field.
Objective: To report successful pregnancy from embryos that had been cryopreserved for 8.9 years.
Design: Case report.