Insights of cellular and molecular changes in sugarcane response to oxidative signaling.

Significant changes in the proteome highlight essential metabolic adaptations for development and oxidative signaling induced by the treatment of young sugarcane plants with hydrogen peroxide. These adaptations suggest that hydrogen peroxide acts not only as a stressor but primarily as a signaling molecule, triggering specific metabolic pathways that regulate growth and plant resilience. Sugarcane is a crucial crop for sugar and ethanol production, often influenced by environmental signals.

Prospecting of the Antioxidant Activity from Extracts Obtained from Chañar () Seeds Evaluated In Vitro and In Vivo Using the Model.

commonly known as Chañar, is a native Chilean plant widely used in folk medicine for its expectorant, pain relief, and antinociceptive properties. This study explored the antioxidant, cytotoxic, and protective effects of its ethanolic (EE) and aqueous (EA) seed extracts against oxidative stress induced by copper sulfate, using both in vitro and in vivo approaches. Phytochemical analyses revealed the presence of phenolic compounds and flavonoids in the extracts.

Unraveling the drought-responsive transcriptomes in nodules of two common bean genotypes during biological nitrogen fixation.

Common bean () can efficiently fix atmospheric nitrogen when associated with Rhizobia. However, drought stress impairs plant metabolic processes, especially the biological nitrogen fixation (BNF). Here, we assessed transcriptional responses in nodules of two common bean genotypes to drought stress under BNF reliance.

Phenotypic Diversity and Genetic Parameters of Clones.

The simultaneous analysis of the maximum number of chemical elements present in plant tissues provides more comprehensive information about their chemical constitution and increases the number of characteristics for the selection process in various plant breeding programs. The objective of this study was to analyze productivity, grain yield, and concentration of chemical elements in tissues of clones to study phenotypic diversity and estimate genetic parameters for use in breeding. This experiment was carried out in Manaus, Amazonas, Brazil, in randomized blocks with four replications.

The Effects of Gluconacin on Bacterial Tomato Pathogens and Protection against , the Causal Agent of Bacterial Spot Disease.

As agricultural practices become more sustainable, adopting more sustainable practices will become even more relevant. Searching for alternatives to chemical compounds has been the focus of numerous studies, and bacteriocins are tools with intrinsic biotechnological potential for controlling plant diseases. We continued to explore the biotechnological activity of the bacteriocin Gluconacin from , PAL5 strain, by investigating this protein's antagonism against important tomato phytopathogens and demonstrating its effectiveness in reducing bacterial spots caused by .

Redox homeostasis at SAM: a new role of HINT protein.

ScHINT1 was identified at sugarcane SAM using subtractive libraries. Here, by bioinformatic tools, two-hybrid approach, and biochemical assays, we proposed that its role might be associated to control redox homeostasis. Such control is important for plant development and flowering transition, and this is ensured with some protein partners such as PAL and SBT that interact with ScHINT1.

Natural Populations of Meyer in Amazonia: Genetic Diversity and Conservation.

, a palm tree incipiently domesticated from upland ecosystems in the Brazilian Amazon, is especially adapted to anthropized areas. The pulp of the fruit, obtained by extractivism, is consumed fresh by the Amazonian population. The objective of the study is to evaluate the diversity and genetic structure of the natural populations of , exploited by extractive farmers in Amazonas, Brazil, seeking to suggest conservation and management strategies for this species.

Transcriptomic Response of the Diazotrophic Bacteria Strain PAL5 to Iron Limitation and Characterization of the Regulatory Network.

has been the focus of several studies aiming to understand the mechanisms behind this endophytic diazotrophic bacterium. The present study is the first global analysis of the early transcriptional response of exponentially growing to iron, an essential cofactor for many enzymes involved in various metabolic pathways. RNA-seq, targeted gene mutagenesis and computational motif discovery tools were used to define the regulon.

[Enterobacter cancerogenus bacteremia in a patient with pelvic trauma].

There are few cases reports of Enterobacter cancerogenus infections. Although it has been isolated in contaminated wounds associated with trauma, it has also been reported as an etiological agent in a wide variety of other infections, with the presence of bacteremia being infrequent. We present the first case reported in Chile of a bacteremia caused by this agent, in a 28-year-old patient with a stable pelvic fracture due a high-energy traffic accident.

Tyrosine Kinase Self-Phosphorylation Controls Exopolysaccharide Biosynthesis in Strain Pal5.

The biosynthesis of exopolysaccharides (EPSs) is essential for endophytic bacterial colonisation in plants bacause this exopolymer both protects bacterial cells against the defence and oxidative systems of plants and acts on the plant colonisation mechanism in . The pathway involved in the biosynthesis of bacterial EPS has not been fully elucidated, and several areas related to its molecular regulation mechanisms are still lacking. relies heavily on EPS for survival indirectly by protecting plants from pathogen attack as well as for endophytic maintenance and adhesion in plant tissues.

Caffeine has a dual influence on NMDA receptor-mediated glutamatergic transmission at the hippocampus.

Caffeine, a stimulant largely consumed around the world, is a non-selective adenosine receptor antagonist, and therefore caffeine actions at synapses usually, but not always, mirror those of adenosine. Importantly, different adenosine receptors with opposing regulatory actions co-exist at synapses. Through both inhibitory and excitatory high-affinity receptors (AR and AR, respectively), adenosine affects NMDA receptor (NMDAR) function at the hippocampus, but surprisingly, there is a lack of knowledge on the effects of caffeine upon this ionotropic glutamatergic receptor deeply involved in both positive (plasticity) and negative (excitotoxicity) synaptic actions.

Dry-Caribbean spp. Strains Ameliorate Drought Stress in Maize by a Strain-Specific Antioxidant Response Modulation.

Drought is a global problem for crop productivity. Therefore, the objective of this research was to evaluate five dry-Caribbean spp. strains in drought stress amelioration in maize plants.

Changes The Molecular Mechanisms of Root Development in L. Growing Under Water Stress.

Background: Inoculation with has shown to influence root development in red rice plants, and more recently, the induced systemic tolerance (IST) response to drought was also demonstrated. The goal of this study was to evaluate the inoculation effect of strain Pal5 on the amelioration of drought stress and root development in red rice ( L.).

New Bacterial Phytase through Metagenomic Prospection.

Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy.

Bilateral Retrobulbar Optic Neuropathy Associated With Golimumab.

We report the first documented case of retrobulbar optic neuropathy associated with golimumab. A 48-year-old man was admitted with a 3-week history of progressive visual loss of his left eye. He had received a second infusion of golimumab for ankylosing spondylitis 10 days before admission.

Hierarchical Classification and System Combination for Automatically Identifying Physiological and Neuromuscular Laryngeal Pathologies.

Objectives: Speech signal processing techniques have provided several contributions to pathologic voice identification, in which healthy and unhealthy voice samples are evaluated. A less common approach is to identify laryngeal pathologies, for which the use of a noninvasive method for pathologic voice identification is an important step forward for preliminary diagnosis. In this study, a hierarchical classifier and a combination of systems are used to improve the accuracy of a three-class identification system (healthy, physiological larynx pathologies, and neuromuscular larynx pathologies).

Liver Angiosarcoma: Rare tumour associated with a poor prognosis, literature review and case report.

Introduction: Liver angiosarcoma is a very uncommon tumour of mesenchymal origin, representing between 0.1-2% of all primary tumours of the liver, affecting mainly men in their sixth or seventh decade of life, with a high mortality in the first years (Chaudhary et al., 2015).

A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues.

Many naturally-occurring cellulolytic microorganisms are not readily cultivable, demanding a culture-independent approach in order to study their cellulolytic genes. Metagenomics involves the isolation of DNA from environmental sources and can be used to identify enzymes with biotechnological potential from uncultured microbes. In this study, a gene encoding an endoglucanase was cloned from red rice crop residues using a metagenomic strategy.

Diagnostic categorization according to the First International Workshop on Ocular Sarcoidosis (FIWOS) criteria in a series of 11 patients.

Objective: Categorization of patients diagnosed with ocular sarcoidosis during the period 2009-2014.

Methods: The medical records of patients with ocular sarcoidosis were reviewed and variables were collected to categorize the patients according to the criteria of the FIWOS.

Results: We found 11 patients, 7 women and 4 men, with sarcoid uveitis; the median age was 58 years.

Colchicine use in isolated renal AA amyloidosis.

We present the case of a 45-year-old woman, with two-year history of chronic renal insufficiency and proteinuria. A kidney biopsy showed the presence of AA amyloidosis (positive Congo red staining and immunohistochemistry). There was no evidence of amyloid deposits in other organs and there was no underlying disease.

Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5.

Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. This acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. It has been demonstrated that exopolysaccharides (EPS), which are produced by strain Pal5 of G.

The bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by Gluconacetobacter diazotrophicus PAL5.

Gluconacetobacter diazotrophicus is an aerobic diazotrophic plant-growth-promoting bacterium isolated from different gramineous plants. We showed that reactive oxygen species (ROS) were produced at early stages of rice root colonization, a typical plant defense response against pathogens. The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G.