Comparative Genomic Study of Vinyl Chloride Cluster and Description of Novel Species, sp. nov.

Advanced physicochemical and chemical absorption methods for chlorinated ethenes are feasible but incur high costs and leave traces of pollutants on the site. Biodegradation of such pollutants by anaerobic or aerobic bacteria is emerging as a potential alternative. Several mycobacteria including L1, NBB4, JS60, NBB3 and JS623 have previously been described as assimilators of vinyl chloride (VC).

Genomic Virulence Features of Two Novel Species sp. nov. and sp. nov., Isolated from Patients with Chronic Pulmonary Diseases.

Strains 335427 and 234509, isolated from two 76-year-old patients with chronic pulmonary diseases, were the subject of polyphasic taxonomic studies and comparative genomic analyses for virulence factors. The 16 rRNA gene sequence similarity between strains 335427 and 234509 and their closest phylogenetic neighbors NBRC 100129 and NBRC 100374 were 99.5% and 100%, respectively.

Cutting the Gordian knot: early and complete amino acid sequence confirmation of class II lasso peptides by HCD fragmentation.

Lasso peptides are a diverse class of ribosomally synthesized and post-translationally modified peptides (RiPPs). Their proteolytic and thermal stability alongside their growing potential as therapeutics has increased attention to these antimicrobial peptides. With the advent of genome mining, the discovery of RiPPs allows for the accurate prediction of putatively encoded structures, however, MS experiments only provide partial sequence confirmation, therefore 2D NMR experiments are necessary for characterisation.

Downsizing Class II Lasso Peptides: Genome Mining-Guided Isolation of Huascopeptin Containing the First Gly1-Asp7 Macrocycle.

A new lasso peptide, huascopeptin, was isolated following genome-mined discovery of a new biosynthetic gene cluster in extremotolerant HST28 from Salar de Huasco, Atacama Desert, Chile. Compound is a 13-residue class II lasso peptide containing a novel Gly1-Asp7 macrolactam ring, a three-residue loop, and a three-residue tail, making it the smallest lasso peptide isolated to date. The lasso structure was confirmed using NOE restraint-based molecular dynamics simulations.

Pseudomonas khazarica sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from Khazar Sea sediments.

A novel Gram-negative, aerobic, motile and rod-shaped bacterium with the potential to biodegrade polycyclic aromatic hydrocarbons, was isolated from Khazar (Caspian) Sea. Strain TBZ2 grows in the absence of NaCl and tolerates up to 8.5% NaCl.

Genomic Insights Into Plant-Growth-Promoting Potentialities of the Genus .

This study was designed to determine the plant growth promoting (PGP) potential of members of the genus . To this end, the genomes of 21 representative strains were examined for genes associated directly or indirectly with plant growth. All of the genomes contained genes that encoded for products associated with the biosynthesis of auxins [indole-3-glycerol phosphate synthases, anthranilate phosphoribosyltransferases (D), anthranilate synthases, and aminases (A and B)], cytokinins (11 well-conserved genes within the predicted biosynthetic gene cluster), siderophores, and nitrogenases ( operon except for atypical ) as well as genes that modulate the effects of biotic and abiotic environmental stress (e.

Streptomyces altiplanensis sp. nov., an alkalitolerant species isolated from Chilean Altiplano soil, and emended description of Streptomyces chryseus (Krasil'nikov et al. 1965) Pridham 1970.

A polyphasic approach was used for evaluating the taxonomic status of strain HST21 isolated from Salar de Huasco in the Atacama Desert. The results of 16S rRNA gene and multilocus sequence phylogenetic analyses assigned strain HST21 to the genus Streptomyceswith Streptomyces albidochromogenes DSM 41800and Streptomyces flavidovirens DSM 40150 as its nearest neighbours. Digital DNA-DNA hydridization (dDDH) and average nucleotide identity (ANI) values between the genome sequences of strain HST21 and S.

Streptomyces huasconensis sp. nov., an haloalkalitolerant actinobacterium isolated from a high altitude saline wetland at the Chilean Altiplano.

Streptomyces strain HST28 isolated from the Salar de Huasco, an athalassohaline and poly-extreme high altitude saline wetland located in northern Chile, was the subject of a polyphasic taxonomic study. Strain HST28 showed morphological and chemotaxonomic features in line with its classification in the genus Streptomyces. Optimal growth of strain HST28 was obtained at 28 °C, pH 8-9 and up to 10 % (w/v) NaCl.