Evaluation and single-laboratory verification of a proposed modification to the U.S. Food and Drug Administration method for detection and identification of Campylobacter jejuni or Campylobacter coli from raw silo milk.

The current U.S. Food and Drug Administration (FDA) methodology for detection of Campylobacter, a leading source for foodborne illness, is outdated.

AOAC International methods committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis.

Responding to a need for a guide for conducting Official Method validation studies of microbiological methods, AOAC utilized the experience of three microbiologists who have been active in the field of method validation. In collaboration, a document was prepared which covered the following areas: terms and their definitions associated with the Official Methods program (e.g.

Enumeration and Differentiation of Vibrio parahaemolyticus and Vibrio vuinificus by DNA-DNA Colony Hybridization Using the Hydrophobic Grid Membrane Filtration Technique for Isolation.

We have developed a means of differentiating and enumerating Vibrio parahaemolyticus and Vibrio vuinificus by DNA-DNA colony hybridization directly on HGMF filters. V. parahaemolyticus can be detected by a -3-radiolabeled gene probe and V.

Campylobacter jejuni in a Washington State Shellfish Growing Bed Associated With Illness.

Consumption of raw Pacific oysters ( Crassotea gigas ) harvested from a Washington State recreational shellfish bed were associated with illness. Illness occurred within 2 d of ingestion of a half-dozen shellstock oysters. Each oyster consist of approximately 20 g of meat.

Incidence of Motile Aeromonads From United States West Coast Shellfish Growing Estuaries.

The distribution of motile Aeromonas species in marine and tributary waters, sediment, and shellfish from 12 major estuarine areas in Washington, Oregon, and California with commercial or sport shellfish harvest was determined during the summer months. Aeromonas spp. were found in half of the total of 400 samples analyzed.

Enumeration of Vibrio Species, Including V. cholerae , from Samples of an Oyster Growing Area, Grays Harbor, Washington.

Water, shellfish, and sediment samples from Grays Harbor, a major commercial oyster producing estuary in the State of Washington, were examined for levels of Vibrio species. Non-01 V. cholerae was found at low levels in 37.

Microbiological Quality of Oysters ( Crassostrea gigas ) and Water of Live Holding Tanks in Seattle, WA Markets.

Oyster ( Crassostrea gigas ) and water samples from Live Holding Tanks at five different Seattle area retail markets were analyzed for microbiological quality indicators and for potential pathogens monthly from March to September, 1987. Aeromonas hydrophilia was the most frequently isolated potential pathogen in this study with a higher incidence in oysters (78%) compared to water (53%). Vibrio cholerae non 01 and V.

Aeromonas hydrophila in Shellfish Growing Waters: Incidence and Media Evaluation.

Levels of Aeromonas hydrophila determined for the shellfish growing area of Grays Harbor, Washington, ranged from 3 to 4600/100 g in oysters and from 3 to 2400/100 ml in water. Of isolates tested, 80% produced a hemolysin, a trait reported to correlate with enterotoxin production and pathogenicity. Two enrichment broths, Tryptic Soy Broth with ampicillin (TSBA) and Modified Rimler Shotts Broth (MRSB) were compared in combination with three solid agar media: Rimler Shotts (RS), Peptone Beef Extract Glycogen (PBG), and MacConkey's (MCA) agars.

Recovery of Aeromonas hydrophila from Oysters Implicated in an Outbreak of Foodborne Illness.

Potentially pathogenic Aeromonas hydrophila organism were isolated from oysters frozen at -72°C for 1-1/2 years. The oysters which had been associated with 472 cases of gastroenteritis in Louisiana in November 1982, were examined and found negative for Salmonella , pathogenic Vibrio parahaemolyticus , and diarrhetic shellfish poison. In 1983, oysters from the same shellfish growing area in Louisiana were implicated in seven cases of gastroenteritis caused by A.

Differentiation of Clostridium perfringens from Related Clostridia in Iron Milk Medium.

The stormy fermentation reaction of Clostridium perfringens in iron milk medium was compared to that of several C. perfringens -like strains. These clostridia, C.

Bacteriological Quality of Fresh Seafood Products from Seattle Retail Markets.

A microbiological survey of 287 (fresh) seafood products from Puget Sound retail markets was conducted over a period of 1 year. The microbiological quality of fresh seafood was high, with only 2.1 % of the samples exceeding the maximum limit for acceptability as suggested by the International Commission on Microbiological Specifications for Foods (ICMSF).