Acaricide resistance in Rhipicephalus microplus collected from selected districts of Madhya Pradesh, Uttar Pradesh and Punjab states of India.

The isolates of Rhipicephalus microplus collected from Madhya Pradesh (MP), Punjab (PJB) and Uttar Pradesh (UP) states of India were characterized using laboratory standardized adult immersion test (AIT) against macrocyclic lactone (ivermectin), synthetic pyrethroids (cypermethrin and deltamethrin), organophosphates (coumaphos and diazinon) and phenylpyrazole compounds (fipronil). Out of the six isolates tested, five isolates except MTH were resistant to deltamethrin and cypermethrin at level II with RF ranging from 16.4 to 24.

Biochemical and computational insights of adenosine deaminase inhibition by Epigallocatechin gallate.

Epigallocatechin gallate, a flavonoid from Camellia sinensis possess various pharmacological activities such as anticancer, antimicrobial and antioxidant etc. Adenosine deaminase, (ADA), is a key enzyme involved in the purine metabolism, the inhibitors of which is being considered as highly promising candidate for the development of anti-proliferative and anti-inflammatory drugs. In this work we studied adenosine deaminase inhibitory activity of epigallocatechin gallate by using biophysical and computational methods.

Selective Activation Re-Mapping Reveals the Mechanism in Apparently Unstable Atrial Tachycardias.

Following atrial fibrillation (AF) ablation procedures, patients may present with atrial tachycardias (ATs) that show remarkable stability for short periods of time but degenerate in unstable forms right afterwards. In order to map these types of ATs, we applied the sequential mapping capabilities only for time segments where ATs exhibited constant cycle length (CL) and activation sequence, excluding the segments with unstable recordings. We herein describe two cases of ATs after AF ablation which were mapped with this technique that allowed for the successful identification and subsequent ablation of the tachycardia circuit.

Selective incorporation of proteinaceous over nonproteinaceous cationic amino acids in model prebiotic oligomerization reactions.

Numerous long-standing questions in origins-of-life research center on the history of biopolymers. For example, how and why did nature select the polypeptide backbone and proteinaceous side chains? Depsipeptides, containing both ester and amide linkages, have been proposed as ancestors of polypeptides. In this paper, we investigate cationic depsipeptides that form under mild dry-down reactions.

A nuanced approach to the Environmental Noise Guidelines for the European Region related to traffic noise.

The recently published guide "Environmental Noise Guidelines for the European Region" provides evidence of the non-auditory effects of environmental noise on population health. This guide reflects the current state of knowledge about how traffic noise affects cardiovascular diseases among others. Despite the valuable contributions of this document in terms of current knowledge, there are different points of improvement in terms of implementation of the guide for all Europe.

A Concurrent Comparison of the Epidemiology and Clinical Presentation of Patients Hospitalized with Pandemic 2009 (H1N1) Influenza and Seasonal Influenza-A in Sub-himalayan Region of Himachal Pradesh.

Background: Pandemic influenza (H1N1) 2009 emerged in April 2009 and spread widely in India. Although an unprecedented number of cases required intensive care, comparative community-based studies with seasonal influenza strains have not shown any significant differences in clinical symptoms or severity.

Methods: The authors performed active surveillance on confirmed influenza-related admissions and compared the clinical profile of patients with pandemic (H1N1) 2009 influenza and patients with seasonal influenza at a tertiary care hospital in Shimla, Himachal Pradesh.

RRI as Diagnostic and Follow up Indicator in Cirrhosis of the Liver with Hepatorenal Syndrome and Refractory Ascites.

Introduction: Hepatorenal syndrome (HRS) is a syndrome of functional renal failure occurring in patients with advanced liver failure in the absence of clinical, laboratory, or histological evidence of other known causes of renal failure. However HRS has always been considered to be potentially reversible. RRI (Renal Resistive Index) is an affordable, non invasive and easily available tool which can be used to detect the early onset of HRS.

The Down Syndrome-Associated Protein, Regulator of Calcineurin-1, is Altered in Alzheimer's Disease and Dementia with Lewy Bodies.

There is a known relationship between Alzheimer's disease (AD) and Down syndrome (DS), with the latter typically developing AD-like neuropathology in mid-life. In order to further understand this relationship we examined intersectin-1 (ITSN1) and the regulator of calcineurin-1 (RCAN1), proteins involved in endosomal and lysosomal trafficking that are over-expressed in DS. We examined RCAN1 and ITSN1 levels (both long (-L) and short (-S) isoforms) and the level of endogenous metals in White Blood Cells (WBCs) collected from AD patients who were enrolled in the Australian Imaging, Biomarker and Lifestyle Study on Ageing (AIBL).

Insights into the flexibility of the T3 loop and GTPase activating protein (GAP) domain of dimeric α and β tubulins from a molecular dynamics perspective.

Tubulin protein is the fundamental unit of microtubules, and comprises of α and β subunits arranged in an alternate manner forming protofilaments. These longitudinal protofilaments are made up of intra- (α-β) and inter-dimer (β-α) interactions. Literature review confirms that GTP hydrolysis results in considerable structural rearrangement within GTP binding site of β-α dimer interface after the release of γ phosphate.

Prevalence and socioeconomic impact of depressive disorders in India: multisite population-based cross-sectional study.

Objectives: The National Mental Health Survey (NMHS) of India was undertaken with the objectives of (1) estimating the prevalence and patterns of various mental disorders in representative Indian population and (2) identifying the treatment gap, healthcare utilisation, disabilities and impact of mental disorders. This paper highlights findings pertaining to depressive disorders (DD) from the NMHS.

Design: Multisite population-based cross-sectional study.

Inkjet Printing of Super Yellow: Ink Formulation, Film Optimization, OLEDs Fabrication, and Transient Electroluminescence.

Inkjet printing technique allows manufacturing low cost organic light emitting diodes (OLEDs) in ambient conditions. The above approach enables upscaling of the OLEDs fabrication process which, as a result, would become faster than conventionally used vacuum based processing techniques. In this work, we use the inkjet printing technique to investigate the formation of thin active layers of well-known light emitting polymer material: Super Yellow (poly(para-phenylene vinylene) copolymer).

Seed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean.

Phytic acid (PA), the major phosphorus reserve in soybean seeds (60-80%), is a potent ion chelator, causing deficiencies that leads to malnutrition. Several forward and reverse genetics approaches have ever since been explored to reduce its phytate levels to improve the micronutrient and phosphorous availability. Transgenic technology has met with success by suppressing the expression of the PA biosynthesis-related genes in several crops for manipulating their phytate content.

Does Kangaroo Mother Care Reduce Anxiety in Postnatal Mothers of Preterm Babies? - A Descriptive Study from a Tertiary Care Centre in South India.

Background: To assess anxiety and depression among postnatal mothers of preterm babies and to evaluate whether Kangaroo mother care reduces their anxiety.

Methods: This descriptive study was conducted in a tertiary care teaching hospital in south India. Anxiety and depression was assessed using Hospital Anxiety and Depression Scale in 2 groups of postnatal mothers (Pre Kangaroo mother care and post Kangaroo mother care) with 50 participants each and compared.

An integrative bioinformatics pipeline to demonstrate the alteration of the interaction between the ALDH2*2 allele with NAD and Disulfiram.

Alcohol use disorder (AUD) is a multifactorial psychiatric behavior disorder. Disulfiram is the first approved drug by the Food and Drug Administration for alcohol-dependent patients, which targets the ALDH2 enzyme. Several genes are known to be involved in alcohol metabolism; mutations in any of these genes are known to be associated with AUD.

An Emerging Nosocomial Pathogen Causing Septicemia in Critically Ill Patients.

Unlabelled: (), is an opportunistic Gram-negative bacteria, normally found in water and soil, notorious for causing nosocomial infections in extremes of ages and immunocompromised patients. It is now emerging as a serious nosocomial pathogen, intrinsically resistant to several commonly used antibiotics (e.g.

Knowledge and perceptions of Indian primary care nurses towards mental illness.

Objectives: To assess nurses' knowledge and perceptions towards mental illness.

Methods: This was a cross-sectional descriptive study conducted among 126 randomly selected nurses those are working under District Mental Health program in Karnataka (India). The data was collected through self-reported questionnaires Using the modified version of Public perception of mental illness questionnaire and Attitude Scale for Mental Illness.

Quercetin binding to Spatholobus parviflorus lectin: Promise of a macromolecular, specific-compound carrier for drug.

Glycan recognition is the most attractive defining feature of lectins, and also they exhibit specific phytochemical interactions at distinct sites without interfering the glycocode recognition capability. These additional sites may be viewed as potential drug carrying sites that could be exploited for targeted drug delivery. The pharmacological effects of quercetin (QN) have already been studied.

Prenatal Diagnosis of a New Case: Balanced Non-Robertsonian Translocation Involving t(15;22)(p11.2;q11.2).

The balanced non-Robertsonian translocation (ROB) associated with acrocentric chromosomes is an unusual phenomenon. We report the case of rare non-ROB involving chromosomes 15 and 22 with cytogenetic and molecular cytogenetic findings of 46,XY,t(15;22)(p11.2;q11.

Myotonic Dystrophy-2: Unusual Phenotype Due to a Small CCTG-expansion.

Myotonic dystrophy type 2 (MD2) is a multisystem disease, predominantly affecting the proximal limb muscles, eyes, endocrine organs, heart and intestines. Longterm asymptomatic creatine kinase (hyper-CKemia) of more than 20 years duration, in association with hyperlipidemia and diabetes, as a manifestation of MD2 has not been reported. A 52-year-old female with a history of hyper-CKemia since the age of 32 years associated with diabetes, hyperlipidemia and hyperuricemia, developed anginal chest pain and proximal muscle weakness together with clinical myotonia when opening the fists at age 51 years.

Developmental, genetic and environmental variations of global DNA methylation in the first leaves emerging from the shoot apical meristem in poplar trees.

In the context of climate changes, clarifying the causes underlying tree phenotypic plasticity and adaptation is crucial. Studies suggest a role of epigenetic mechanisms in response to external stimuli, raising the question whether such processes can promote acclimation of trees exposed to adverse climate conditions. Recently, we revealed an environmental epigenetic footprint in the shoot apical meristem (SAM) which could partially be transmitted mitotically, for several months, up until the winter-dormant bud in field conditions.

Novel Fibrinolytic Protease Producing VITSD8 from Marine Sponges.

Fibrinolytic enzymes have received more attention due to their medicinal potential for thrombolytic diseases. The aim of this study is to characterize the in vitro fibrinolytic nature of purified protease producing VITSD8 from marine brown tube sponges . Three varieties of sponge were collected from the Rameshwaram Sea coast, Tamil Nadu, India.

NaS-promoted reduction of azides in water: synthesis of pyrazolopyridines in one pot and evaluation of antimicrobial activity.

Reduction of various azides using Na2S has been accomplished in water, and, in situ, the resulting amines on reaction with various ketones lead to pyrazolo[3,4-b]pyridines in one pot. Thus, a number of new trifluoromethyl-substituted pyrazolo[3,4-b]pyridine compounds have been prepared and screened for antimicrobial activity against different Gram-positive and Gram-negative strains. A good number of compounds, 4a, 4b, 4d, 4f, 4i, 4k, 4l, 4m, 4r and 4s, were found to possess promising activity.