Calvarial hyperostosis syndrome in a Dalmatian dog.

Key Clinical Message: Calvarial hyperostosis syndrome is an uncommon and self-limiting disease affecting juvenile dogs. Only symptomatic treatment has been described, and diagnosis is based on clinical findings, imaging, and disease progression.

Abstract: This is the first reported case of calvarial hyperostosis syndrome in a Dalmatian dog.

In vitro bioassays for monitoring drinking water quality of tap water, domestic filtration and bottled water.

Background: Location-specific patterns of regulated and non-regulated disinfection byproducts (DBPs) were detected in tap water samples of the Barcelona Metropolitan Area. However, it remains unclear if the detected DBPs together with undetected DPBs and organic micropollutants can lead to mixture effects in drinking water.

Objective: To evaluate the neurotoxicity, oxidative stress response and cytotoxicity of 42 tap water samples, 6 treated with activated carbon filters, 5 with reverse osmosis and 9 bottled waters.

A proof-of-concept study to investigate the efficacy of heat-inactivated autovaccines in Mycobacterium caprae experimentally challenged goats.

This study aimed to assess the efficacy of a heat-inactivated Mycobacterium caprae (HIMC) vaccine in goats experimentally challenged with the same strain of M. caprae. Twenty-one goats were divided into three groups of seven: vaccinated with heat-inactivated Mycobacterium bovis (HIMB), with HIMC and unvaccinated.

Insights to estimate exposure to regulated and non-regulated disinfection by-products in drinking water.

Background: Knowledge about human exposure and health effects associated with non-routinely monitored disinfection by-products (DBPs) in drinking water is sparse.

Objective: To provide insights to estimate exposure to regulated and non-regulated DBPs in drinking water.

Methods: We collected tap water from homes (N = 42), bottled water (N = 10), filtered tap water with domestic activated carbon jars (N = 6) and reverse osmosis (N = 5), and urine (N = 39) samples of participants from Barcelona, Spain.

Simultaneous analysis of 11 haloacetic acids by direct injection-liquid chromatography-electrospray ionization-triple quadrupole tandem mass spectrometry and high resolution mass spectrometry: occurrence and evolution in chlorine-treated water.

A fast, simple, selective, and sensitive method for the analysis of 11 haloacetic acids (HAAs) in chlorine-treated water has been developed. The method is based on liquid chromatography-electrospray ionization-triple quadrupole tandem mass spectrometry (LC/ESI-QqQ-MS/MS) with direct injection of the aqueous sample. The main novelty of this method over the previously published procedures based on different techniques of mass spectrometry with direct injection is the combination of the simultaneous analysis of three types of HAAs (chlorinated, brominated, and iodinated) with its simplicity and low LODs (0.

Analysis of 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and its brominated analogues in chlorine-treated water by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS).

A simple, selective and sensitive method for the analysis of the strong mutagen 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) and its brominated analogues (BMXs) in chlorine-treated water has been developed. The method is based on gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS), previous liquid-liquid extraction (LLE) of a smaller sample volume compared to other methods and on-line derivatization with a silylation reactive. GC-QqQ-MS/MS has been raised as an alternative easier to perform than gas chromatography coupled to high resolution mass spectrometry (GC-HRMS) for the analysis of MX and BMXs, and it allows to achieve low LODs (0.

Concentrations of organochlorine pesticides and 2,4,6-trichloroanisole in cork bark.

Organochlorine pesticides are persistent lipophilic organic pollutants and tend to accumulate in growing plants. During growth, cork is in contact with the open air for long periods (9-12 years). Owing to the previous widespread use of organochlorine pesticides and their high persistence in the environment, there is a risk that residues of such pesticides may be present in cork.

Analysis of nitrosamines in water by automated SPE and isotope dilution GC/HRMS Occurrence in the different steps of a drinking water treatment plant, and in chlorinated samples from a reservoir and a sewage treatment plant effluent.

A method based on automated solid-phase extraction (SPE) and isotope dilution gas chromatography/high resolution mass spectrometry (GC/HRMS) has been developed for the analysis of nine nitrosamines in water samples. The combination of automated SPE and GC/HRMS for the analysis of nitrosamines has not been reported previously. The method shows as advantages the selectivity and sensitivity of GC/HRMS analysis and the high efficiency of automated SPE with coconut charcoal EPA 521 cartridges.

Analysis of pesticides and metabolites in Spanish surface waters by isotope dilution gas chromatography/mass spectrometry with previous automated solid-phase extraction Estimation of the uncertainty of the analytical results.

A method based on isotope dilution gas chromatography/mass spectrometry (GC/MS) with automated solid-phase extraction (SPE) is described for the analysis of 32 pesticides and metabolites in surface waters. This approach consist in the use of nine isotopically labelled representative pesticides as internal standards, which allows high accuracy (trueness and precision) and sensitivity for most analysed compounds, as it is required for isotope dilution-based methods. Uncertainties associated with pesticide determination in real samples were estimated using quality assurance/quality control (QA/QC) data.

Analysis of alkyl and 2-6-ringed polycyclic aromatic hydrocarbons by isotope dilution gas chromatography/mass spectrometry. Quality assurance and determination in Spanish river sediments.

An accurate, precise and sensitive method is described for the analysis of 29 polycyclic aromatic hydrocarbons (PAHs), including 19 2-6-ringed PAHs and 10 alkyl-PAHs. The method is based on an isotope dilution technique using gas chromatography/mass spectrometry (GC/MS) and available labeled PAHs as internal standards. Quality parameters were calculated with satisfactory results and 36 Spanish river sediments were analysed.

Simultaneous quantitative analysis of anionic, cationic, and nonionic surfactants in water by electrospray ionization mass spectrometry with flow injection analysis.

A rapid method is described for the quantitative analysis of anionic, cationic, and nonionic surfactants in water samples by flow injection analysis coupled to electrospray ionization mass spectrometry (FIA/ESI-MS). All surfactants were isolated by liquid-liquid extraction and quantified using labeled triethoxylated nonylphenol ([13C6]-NP3EO) and sodium dibutylnaphthalenesulfonate as internal standards. FIA/ESI-MS was performed by alternating both positive and negative ionization modes, which allows simultaneous analysis of most common surfactants in a short time.