Publications by authors named "Carleen Van Siclen"
Objective: Using a US Food and Drug Administration (FDA) emergency use authorization (EUA) reverse transcription polymerase chain reaction (RT-PCR) method, we examined the analytic performance accuracy of saliva specimens as compared to nasopharyngeal (NP) specimens in symptomatic patients. Correlation between test results and symptoms was also evaluated.
Methods: Over a 5-week period in 2020, 89 matched saliva and nasopharyngeal swabs were collected from individuals exhibiting symptoms consistent with SARS-CoV-2.
Objective: The ongoing COVID-19 pandemic caused by SARS-CoV-2 has challenged diagnostic laboratories to re-examine traditional methods for collecting specimens and sample types used in molecular testing. Our goal was to demonstrate that saliva can be used for detecting SARS-CoV-2 and correlates well with established molecular methods using nasopharyngeal (NP) swabs.
Methods: We examined use of a saliva collection device in conjunction with a laboratory-developed real-time reverse transcription-polymerase chain reaction (LDPCR) method for detecting SARS-CoV-2 in a symptomatic population and compared results with 2 US Food and Drug Administration (FDA)-approved methods (emergency use authorization [EUA]) that use specimens from NP swabs.
Patient: A 53-year-old man, ethnicity unknown.
Chief Complaint: Leg swelling and pain.
History Of Present Illness: The patient has a history of atrial fibrillation that had been treated with 3 separate cardiac ablations, most recently in the year 2014.
Clinical History Patient: 37-year-old man of half African and half Italian ethnicity.
Chief Complaint: Sickle cell crisis (SCC).
History Of Present Illness: The patient had severe pain in his lower back that radiated to both thighs.