Publications by authors named "Carla L Freitas"
Background: The current study mainly focused on provide further insights into the association of the miR-22-3p and miR-29c-3p expression in CFU-Hill colonies with birth weight and senescence process in children.
Methods: This cross-sectional study evaluated 61 children (32 boys, 29 girls). The CFU-Hill colonies number was evaluated in vitro by cell culture technique and senescence was detected by β-galactosidase (SA-β-Gal) assay.
Human Fallopian Tube - Derived Mesenchymal Stem Cells Inhibit Experimental Autoimmune Encephalomyelitis by Suppressing Th1/Th17 Activation and Migration to Central Nervous System.
Stem Cell Rev Rep
February 2022
Article Synopsis
- Mesenchymal stem cells (MSCs) are undifferentiated cells found in adult tissues that show promise for treating various diseases due to their ability to renew and differentiate into different cell types while modulating immune responses.
- This study focused on human tubal mesenchymal stem cells (htMSCs) and their effects in an experimental autoimmune encephalomyelitis (EAE) model, revealing their ability to suppress the activation of dendritic cells and promote anti-inflammatory cytokine release.
- Results indicated that htMSCs lead to milder disease symptoms, with reduced immune cell infiltration and increased levels of protective factors, suggesting their potential as a therapy for inflammatory and neurodegenerative conditions.
Zika virus (ZIKV) has the ability to cross placental and brain barriers, causing congenital malformations in neonates and neurological disorders in adults. However, the pathogenic mechanisms of ZIKV-induced neurological complications in adults and congenital malformations are still not fully understood. Gas6 is a soluble TAM receptor ligand able to promote flavivirus internalization and downregulation of immune responses.
View Article and Find Full Text PDFCorrigendum: Protective Immunity to Dengue Virus Induced by DNA Vaccines Encoding Nonstructural Proteins in a Lethal Challenge Immunocompetent Mouse Model.
Front Med Technol
November 2020
[This corrects the article DOI: 10.3389/fmedt.2020.
View Article and Find Full Text PDFProtective Immunity to Dengue Virus Induced by DNA Vaccines Encoding Nonstructural Proteins in a Lethal Challenge Immunocompetent Mouse Model.
Front Med Technol
October 2020
Dengue virus represents the main arbovirus affecting humans, but there are no effective drugs or available worldwide licensed vaccine formulations capable of conferring full protection against the infection. Experimental studies and results generated after the release of the licensed anti-DENV vaccine demonstrated that induction of high-titer neutralizing antibodies does not represent the sole protection correlate and that, indeed, T cell-based immune responses plays a relevant role in the establishment of an immune protective state. In this context, this study aimed to further demonstrate protective features of immune responses elicited in immunocompetent C57BL/6 mice immunized with three plasmids encoding DENV2 nonstructural proteins (NS1, NS3, and NS5), which were subsequently challenged with a DENV2 strain naturally capable of inducing lethal encephalitis in immunocompetent mouse strains.
View Article and Find Full Text PDFIn the present study, we evaluated the immunological responses induced by dengue vaccines under experimental conditions after delivery via a transcutaneous (TC) route. Vaccines against type 2 Dengue virus particles (DENV2 New Guinea C (NGC) strain) combined with enterotoxigenic (ETEC) heat-labile toxin (LT) were administered to BALB/c mice in a three-dose immunization regimen via the TC route. As a control for the parenteral administration route, other mouse groups were immunized with the same vaccine formulation via the intradermic (ID) route.
View Article and Find Full Text PDFZika virus congenital syndrome: experimental models and clinical aspects.
J Venom Anim Toxins Incl Trop Dis
September 2017
Article Synopsis
- Viral infections like yellow fever, H1N1, and HIV significantly impact global health, causing millions of deaths and substantial economic costs.
- The Zika virus (ZIKV), initially thought to be harmless, gained attention after a surge in cases of microcephaly in babies and Guillain-Barré syndrome in adults, prompting a Public Health Emergency declaration by the WHO in 2016.
- This review explores the clinical aspects of congenital Zika syndrome (CZS), including its severe symptoms like microcephaly and ocular damage, and connects these with findings from experimental animal models.
Article Synopsis
- Zika virus (ZIKV), first identified in 1947, became a significant public health concern after causing major outbreaks from 2007 onward, linked to various symptoms including fever and rash.
- The virus, particularly the Brazilian strain (ZIKV(BR)), has been associated with severe birth defects such as microcephaly, although direct experimental proof of this connection has been lacking until now.
- New research demonstrates that ZIKV(BR) infects developing fetuses and human brain cells, leading to cell death and impaired neurodevelopment, supporting the connection between the virus and congenital malformations.
Objective:: To assess the knowledge and practice of pediatricians about infants with physiological reflux and gastroesophageal reflux disease.
Methods:: 140 pediatricians were interviewed during two scientific events in 2009 and 2010. The questions referred to two clinical cases of infants.