Publications by authors named "Carla Gelle"
Background: BIN1 is a major susceptibility gene for AD and BIN1 protein interacts with Tau. However, the contribution of BIN1 and its isoforms to AD pathogenesis remains unclear. We recently described that human BIN1 isoform1 (BIN1iso1) induces an accumulation of early endosome vesicles leading to neurodegeneration in Drosophila retina and that the early endosome size regulation was conserved in human induced neurons.
View Article and Find Full Text PDFAlzheimer's disease (AD) is a neurodegenerative disease and the most frequent cause of dementia. It is characterized by the accumulation in the brain of two pathological protein aggregates: amyloid-β peptides (Aβ) and abnormally phosphorylated tau. The progressive cognitive decline observed in patients strongly correlates with the synaptic loss.
View Article and Find Full Text PDFArticle Synopsis
- The synapse is critical for neuronal communication and is impacted early in neurodegenerative diseases, making it essential to study synapse loss.
- A new high-content screening (HCS) model was developed to quantitatively analyze how gene silencing affects synaptic density using primary neuronal cultures from neonatal rat hippocampus.
- The study details various protocols for culturing neurons, transducing lentiviral shRNAs, immunostaining, image acquisition, and analyzing synaptic density in a streamlined, automated 384-well plate format.