Long-distance translocation of protein during morphogenesis of the fruiting body in the filamentous fungus, Agaricus bisporus.

Commercial cultivation of the mushroom fungus, Agaricus bisporus, utilizes a substrate consisting of a lower layer of compost and upper layer of peat. Typically, the two layers are seeded with individual mycelial inoculants representing a single genotype of A. bisporus.

Mushroom (Agaricus bisporus).

We have devised an easy and effective genetic transformation method for the preeminent edible mushroom, Agaricus bisporus. Our method exploits the T-DNA transfer mechanism in Agrobacterium tumefaciens and relies on the reproductive fruiting body as the recipient tissue. The use of fruiting body explants, particularly the gill, provided high-frequency transformation, overcoming the inefficacy of Agrobacterium-based methods targeting fungal spores or vegetative mycelium.

Ozone-induced ethylene and foliar injury responses are altered in 1-aminocyclopropane-1-carboxylate synthase antisense potato plants.

•  In potato (Solanum tuberosum), two 1-aminocyclopropane-1-carboxylate synthase (ACS) genes are induced by ozone (O ). Antisense inhibition of these ethylene (C H ) biosynthetic genes allowed us to examine the relationship between O -induced C H emission and foliar injury. •  Thirty-two lines (antisense for ST-ACS4 or ST-ACS5) were screened in the glasshouse for acute O -induced C H and lesions.