Fatty Acid Composition by Total Acyl Lipid Collision-Induced Dissociation Time-of-Flight (TAL-CID-TOF) Mass Spectrometry.

Total acyl lipid collision-induced dissociation time-of-flight (TAL-CID-TOF) mass spectrometry uses a quadrupole time-of-flight (QTOF) mass spectrometer to rapidly provide a comprehensive fatty acid composition of a biological lipid extract. Samples are infused into a QTOF instrument, operated in negative mode, and the quadrupole is used to transfer all, or a wide mass range of, precursor ions to the collision cell for fragmentation. Time-of-flight-acquired mass spectra provide mass accuracy and resolution sufficient for chemical formula determination of fatty acids in the complex mixture.

A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition.

Lipid changes that occur in leaves of plants (e.g., Arabidopsis thaliana), during cold and freezing stress can be analyzed with electrospray ionization triple quadrupole mass spectrometry, using high-throughput multiple reaction monitoring (MRM).

LipidomeDB Data Calculation Environment Has Been Updated to Process Direct-Infusion Multiple Reaction Monitoring Data.

LipidomeDB Data Calculation Environment (DCE), a web application for processing data from direct-infusion tandem mass spectrometer data on lipids, was described by Zhou et al. (, Lipids, 46, 879-884). The original version processed multiple precursor and/or neutral loss scans on multiple samples.