Publications by authors named "Carl Dargitz"
Article Synopsis
- * Current techniques, like flow cytometry, have limitations such as high sample input, low throughput, and standardization challenges, hindering proper characterization of cell therapy products.
- * The described assays utilize isolated genomic DNA to identify and quantify specific immune cell types through DNA methylation patterns, enabling high throughput and robust testing for T cells and potentially improving cell therapy processes.
Article Synopsis
- Induced pluripotent stem cells (iPSCs) are valuable for studying diseases, discovering drugs, and regenerative medicine, but creating high-quality clinical-grade iPSCs is still challenging.
- The CTS CytoTune-iPS 2.1 kit is the first off-the-shelf solution designed for clinical and translational research, aiming to streamline the reprogramming process.
- The kit enables the consistent generation of iPSCs from various cell types, and the resulting clones are thoroughly characterized to ensure they are pluripotent, maintain genomic integrity, and are suitable for clinical use.
Large-scale collections of induced pluripotent stem cells (iPSCs) could serve as powerful model systems for examining how genetic variation affects biology and disease. Here we describe the iPSCORE resource: a collection of systematically derived and characterized iPSC lines from 222 ethnically diverse individuals that allows for both familial and association-based genetic studies. iPSCORE lines are pluripotent with high genomic integrity (no or low numbers of somatic copy-number variants) as determined using high-throughput RNA-sequencing and genotyping arrays, respectively.
View Article and Find Full Text PDFLung disease is a major cause of death in the United States, with current therapeutic approaches serving only to manage symptoms. The most common chronic and life-threatening genetic disease of the lung is cystic fibrosis (CF) caused by mutations in the cystic fibrosis transmembrane regulator (CFTR). We have generated induced pluripotent stem cells (iPSCs) from CF patients carrying a homozygous deletion of F508 in the CFTR gene, which results in defective processing of CFTR to the cell membrane.
View Article and Find Full Text PDFDespite therapeutic advancement, pulmonary disease still remains a major cause of morbidity and mortality around the world. Opportunities to study human lung disease either in vivo or in vitro are currently limited. Using induced pluripotent stem cells (iPSCs), we generated mature multiciliated cells in a functional airway epithelium.
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