Publications by authors named "Cardarelli M"

Rising temperatures due to climate change may affect the quality of open-field cultivated processing tomatoes by altering the nutrient content. Bioinoculants are growing in popularity as a nature-based strategy to mitigate these environmental stresses. Untargeted quantitative NMR spectroscopy was leveraged to characterize the metabolome of tomato fruits exposed to abiotic stress during the year 2022, which was marked by unexpected high temperatures and low rainfall compared to the year 2021 with average conditions.

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Introduction: Considering that plant biostimulants can be sprayed multiple times on leafy crops even just before harvest, it is relevant to know the impact of biostimulant applications on population dynamics of lettuce leaves to ensure food safety. Two trials were carried out to investigate whether the applications of a seaweed extract and a vegetal-derived protein hydrolysate (PH) could affect the growth in shake flasks (Exp. 1) and plant growth and survival of artificially inoculated on the leaf surface of lettuce grown in a floating system (Exp.

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Background: Vegetal-derived protein hydrolysates (PHs) have been recognized as sustainable biostimulant products due to their beneficial effects on crops. However, most studies on PHs have been conducted at a fixed ratio of nitrate-to-ammonium (NO :NH ) without considering other N application scenarios, leading to inconsistent results among the studies. This study compared the influences of N levels (2 or 10 mM N), NO:NH ratios (100:0, 75:25, 50:50, or 25:75), and PH application methods - control, foliar spray (PH-F) or root application (PH-R) - on the yield, morphology, nutrients, and nutraceutical quality of hydroponic lettuce.

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The timing and efficiency of arsenic (As) accumulation is crucial for using the hyperaccumulator P. vittata in remediation of As-contaminated soils. In this study, through an innovative microXRF-based approach, using a new "pinna powder" sampling method, we monitored As accumulation over time in fronds of individual P.

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This greenhouse study evaluated the effects of soil enrichment with rhizosphere bacteria on the growth and accumulation of arsenic in grown on a naturally As-rich soil. Inoculations were performed with a consortium of six bacteria resistant to 100 mM arsenate and effects were compared to those obtained on the sterilized soil. Selected bacteria from the consortium were also utilized individually: PVr_9 homologous to that produces IAA and siderophores and shows ACC deaminase activity, PVr_15 homologous to that contains the arsenate reductase gene, and PVr_5 homologous to that possesses all traits from both PVr_9 and PVr_15.

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The ancient native variety of elephant garlic, known as "Aglione della Valdichiana" and cultivated in the Valdichiana area of Tuscany, Italy, has gained recent recognition in the National Catalog of Local Varieties. The renewed interest in traditional products has led to a focus on identifying local varieties of elephant garlic, driven by their distinctive organoleptic and nutritional characteristics. However, other types of elephant garlic nowadays available on the market appear similar, but challenges exist in discerning their origin and composition.

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The need to increase agricultural production to feed a steadily growing population may clash with the more environmentally friendly but less efficient production methods required. Therefore, it is important to try to reduce the use of chemical inputs without compromising production. In this scenario, natural biostimulants have become one of the most sought-after and researched technologies.

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Background: Protein hydrolysates (PHs) can enhance plant nitrogen nutrition and improve the quality of vegetables, depending on their bioactive compounds. A tomato greenhouse experiment was conducted under both optimal (14 mM) and suboptimal (2 mM) nitrogen (N-NO) conditions. Tomatoes were treated with a new Malvaceae-derived PH (MDPH) and its molecular fractions (MDPH1, >10 kDa; MDPH2, 1-10 kDa and MDPH3, <1 kDa).

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Article Synopsis
  • Protein hydrolysates (PH) biostimulants can boost crop growth and resilience, especially in plants facing nitrogen limitations.
  • Research focused on how different molecular fractions of PH affected lettuce plants under varying nitrogen levels, revealing increases in biomass and hormone production linked to auxin and cytokinin.
  • The study also highlighted that PH and its lighter fraction (PH3) promoted cell wall growth and enhanced antioxidant responses, indicating that lighter molecular fractions might offer greater bioactivity.
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Plant biostimulants are widely applied in agriculture for their ability to improve plant fitness. In the present work, the impact of Graminaceae-derived protein hydrolysate (P) and its lighter molecular fraction F3 (< 1 kDa) on lettuce plants, subjected to either no salt or high salt conditions, was investigated through the combination of metabolomics and transcriptomics. The results showed that both treatments significantly modulated the transcriptome and metabolome of plants under salinity stress, highlighting an induction of the hormonal response.

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Article Synopsis
  • This study compared robotic-assisted varicocelectomy (RAV) and laparoscopic varicocelectomy (LV) in 40 pediatric patients over two years, focusing on techniques and outcomes.
  • Results showed that while both methods were effective, LV had a significantly shorter average operative time (20 min) compared to RAV (34.5 min), better cosmetic results, and lower total costs (€1,587.07 vs. €5,650.31).
  • The conclusion indicates that while RAV is a safe option, laparoscopic techniques are preferable for treating pediatric varicocele due to their efficiency and cost-effectiveness.
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Introduction: High-throughput phenotyping technologies together with metabolomics analysis can speed up the development of highly efficient and effective biostimulants for enhancing crop tolerance to drought stress. The aim of this study was to examine the morphophysiological and metabolic changes in tomato plants foliarly treated with two protein hydrolysates obtained by enzymatic hydrolysis of vegetal proteins from Malvaceae (PH1) or Fabaceae (PH2) in comparison with a control treatment, as well as to investigate the mechanisms involved in the enhancement of plant resistance to repeated drought stress cycles.

Methods: A phenotyping device was used for daily monitoring morphophysiological traits while untargeted metabolomics analysis was carried out in leaves of the best performing treatment based on phenotypic results.

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spp. are widely reported to regulate plant growth by improving nutrient uptake, photosynthesis, and abiotic stress tolerance. However, their possible application for bedding plants is little explored, especially when comparing different growing media.

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Background: Microgreens constitute dietary sources of bioactive compounds imparting numerous health benefits and enhancing sensory experience. They can be successfully cultivated in soilless systems where biostimulants can be easily integrated as seed-priming and post-germination agents improving the sustainability of a crop's final production. Compared to an untreated control, three priming agents (a commercial legume-derived protein hydrolysate (A250), a novel protein hydrolysate derived from peanut biomass (H250) and hydropriming (HO)) were applied to Komatsuna and Mibuna seeds grown as microgreens and compared for their effects on yield parameters, mineral composition, ABTS and FRAP antioxidant capacity, carotenoid concentration and phenolic compounds.

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The demand for high-quality strawberries continues to grow, emphasizing the need for innovative agricultural practices to enhance both yield and fruit quality. In this context, the utilization of natural products, such as biostimulants, has emerged as a promising avenue for improving strawberry production while aligning with sustainable and eco-friendly agricultural approaches. This study explores the influence of a bacterial filtrate (BF), a vegetal-derived protein hydrolysate (PH), and a standard synthetic auxin (SA) on strawberry, investigating their effects on yield, fruit quality, mineral composition and metabolomics of leaves and fruits.

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Although entirely preventable, rheumatic heart disease (RHD), a disease of poverty and social disadvantage resulting in high morbidity and mortality, remains an ever-present burden in low-income and middle-income countries (LMICs) and rural, remote, marginalised and disenfranchised populations within high-income countries. In late 2021, the National Heart, Lung, and Blood Institute convened a workshop to explore the current state of science, to identify basic science and clinical research priorities to support RHD eradication efforts worldwide. This was done through the inclusion of multidisciplinary global experts, including cardiovascular and non-cardiovascular specialists as well as health policy and health economics experts, many of whom also represented or closely worked with patient-family organisations and local governments.

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Salinity or salt stress has deleterious effects on plant growth and development. It imposes osmotic, ionic, and secondary stresses, including oxidative stress on the plants and is responsible for the reduction of overall crop productivity and therefore challenges global food security. Plants respond to salinity, by triggering homoeostatic mechanisms that counter salt-triggered disturbances in the physiology and biochemistry of plants.

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In addition to be used as a plant protection agent, copper (Cu) is also an essential micronutrient for plant growth and development. The bioavailability of Cu in agricultural systems can be limited due to its specific physical-chemical characteristics, leading to imbalances in plant production. To address this issue, an experimental trial was conducted on Genovese basil ( L.

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The global of paediatric and congenital heart disease (PCHD) is substantial. We propose a novel public health framework with recommendations for developing effective and safe PCHD services in low-income and middle-income countries (LMICs). This framework was created by the Global Initiative for Children's Surgery Cardiac Surgery working group in collaboration with a group of international rexperts in providing paediatric and congenital cardiac care to patients with CHD and rheumatic heart disease (RHD) in LMICs.

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Salinity in water and soil is a critical issue for food production. Using biostimulants provides an effective strategy to protect crops from salinity-derived yield losses. The research supports the effectiveness of protein hydrolysate (PH) biostimulants based on their source material.

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Protein hydrolysate biostimulants are environmentally friendly options for the reduction of nitrogen input, but their plant growth-promoting mechanisms are still not completely unveiled. Here, to put the “signaling peptide theory” to the test, a greenhouse experiment was undertaken using low (1 mM) and optimal (8 mM) NO3-treated butterhead lettuce and three molecular fractions (PH1 (>10 kDa), PH2 (1−10 kDa) and PH3 (<10 kDa) fractions), in addition to the whole product Vegamin®: PH, in a randomized block design. PH1 and PH3 significantly increased fresh yield (+8%) under optimal (lighter leaves), but not under low (darker leaves) NO3 conditions.

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Three commercial microbial-based biostimulants containing fungi (arbuscular mycorrhizae and spp.) and other microrganisms (plant growth-promoting bacteria and yeasts) were applied on a processing tomato crop in a two-year field experiment in southern Italy. The effects of the growing season and the microorganism-based treatments on the yield, technological traits and functional quality of the tomato fruits were assessed.

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Auxin Response Factor 8 plays a key role in late stamen development: its splice variants ARF8.4 and ARF8.2 control stamen elongation and anther dehiscence.

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Article Synopsis
  • Protein hydrolysates (PHs) are plant biostimulants made from amino acids and peptides derived from protein sources, but their effects on cut flowers are not well-studied.
  • In research involving two chrysanthemum cultivars, plant-derived PHs significantly increased fresh plant biomass and improved overall plant health compared to controls, while the animal-derived PH had less positive effects.
  • Notably, certain PH treatments affected nutrient concentration in leaves and flowers, influenced flower stem longevity, and the ability of flowers to retain water during vase life was linked to nutrient transport issues rather than salt buildup.
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Salinity stress is a major constraint to sustainable crop production due to its adverse impact on crop growth, physiology, and productivity. As potato is the fourth most important staple food crop, enhancing its productivity is necessary to ensure food security for the ever-increasing population. Identification and cultivation of salt-tolerant potato genotypes are imperative mitigating strategies to cope with stress conditions.

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