Single-cell HER2 quantification via instant signal amplification in microdroplets.

Accurate and ultrasensitive evaluation of human epidermal growth factor receptor 2 (HER2) protein is key to early diagnosis and subtype differentiation of breast cancer. Single-cell analyses to reduce ineffective targeted therapies due to breast cancer heterogeneity and improve patient survival remain challenging. Herein, we reported a novel droplet microfluidic combined with an instant cation exchange signal amplification strategy for quantitative analysis of HER2 protein expression on single cells.

All-fiber online Raman sensor with enhancement via a Fabry-Perot cavity.

In this Letter, a novel all-fiber online Raman sensor with significant signal enhancement via a Fabry-Perot (FP) cavity is proposed and demonstrated. The FP cavity structure is formed by inserting a long-pass coated fiber and a gold-plated capillary into a silver-lined capillary with a gap. A corroded single-mode fiber is inserted into the gold-plated capillary to guide the excitation light into the FP cavity.

Volumetric enhancement of Raman scattering for fast detection based on a silver-lined hollow-core fiber.

Fast detection and identification of chemicals are of utmost importance for field testing and real-time monitoring in many fields. Raman spectroscopy is the predominant technique in principle, but its wide application is limited on account of weak scattering efficiency. Surface Enhanced Raman Spectroscopy (SERS) technique provides a solution for signal enhancement, but may not good at fast detection due to cross contamination and bulky instruments.