Publications by authors named "Caoimhe Morley"
Article Synopsis
- The study investigates various methods for whole genome amplification in the analysis of somatic mutations, specifically copy number variants (CNVs), in human brain tissue.
- Three techniques are compared: PicoPLEX, primary template-directed amplification (PTA), and droplet MDA, revealing distinct characteristics of each method in terms of amplification efficiency and chimeric profiles.
- The research confirms that a significant portion of brain cells (20.6%) exhibit CNVs, emphasizing the need for careful selection of amplification methods and reference genomes when studying genomic variations in both healthy and diseased brains.
The presence of somatic mutations, including copy number variants (CNVs), in the brain is well recognized. Comprehensive study requires single-cell whole genome amplification, with several methods available, prior to sequencing. We compared PicoPLEX with two recent adaptations of multiple displacement amplification (MDA): primary template-directed amplification (PTA) and droplet MDA, across 93 human brain cortical nuclei.
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