[Experiment research on the location of DNA lesion in the nucleic acid sequence].

Objective: To research on establishing a method that can detect the DNA lesion at the level of nucleic acid.

Methods: The TK6 cell was cultured and treated, the genomic DNA was extracted, and the strand cleavage was induced by the endonuclease Hinf I. Then the genomic DNA was amplified by randomized terminal linker-dependent PCR (RDPCR), and hybridized by Southern hybridization with the single-stranded probes of the exon 2 of k-ras gene.