Aspartate aminotransferase-to-platelet ratio index as a novel predictor of early mortality in heat stroke patients: a multi-centre retrospective study.

Background: The aspartate aminotransferase-to-platelet ratio index (APRI) is an effective non-invasive marker for chronic liver dysfunction. Given that heat stroke patients often suffer from poor prognosis due to multi-organ involvement, with liver injury and coagulation dysfunction being of particular concern, this study aims to investigate whether APRI can comprehensively reflect liver injury and coagulation dysfunction in heat stroke patients and explore its relationship with 28-day mortality.

Methods: This retrospective study analysed electronic medical records from patients treated at 57 grade A tertiary hospitals in China from May 2005 to May 2024.

Assessment of intraorbital hydroxyapatite implant exposure beyond 10 years of implantation.

Background: To analyze intraorbital Hydroxyapatite(HA) implant exposure occurring beyond ten years of implantation and suggest potential contributing factors.

Design: A retrospective cohort study based in the Shanghai Ninth People's Hospital.

Methods: One hundred and one Chinese patients with HA implant exposure were identified, from which 38 patients with implants exposure after ten years of insertion were selected and analyzed for demographic and medical records, clinical manifestations, surgery type, implant size, exposure area, follow-up duration, complications, details regarding prosthesis care, and treatment methods.

CVA16 infection causes neurological injury by engaging TLR2/MYD88/TNF-α/CXCL1 signalling pathway in hSCARB2 knock-in mice.

Coxsackievirus A16 (CVA16), a major pathogen responsible for hand-foot-and-mouth disease (HFMD) in children, has frequently replaced Enterovirus A71 as the predominant causative agent in China and other Asia-Pacific regions. The lack effective drugs and vaccines against this virus exacerbates the concerns on its outbreaks. Clinical reports and laboratory studies indicate that CVA16 infection may lead to neurological injury, but the precise mechanisms remain elusive.

Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens.

The antitumor drug candidate, loonamycin, was isolated from Nocardiopsis flavescens NA01583 and exerts its antitumor activity by inhibiting topoisomerase I activity. However, the current production levels of loonamycin are insufficient for further research. To address this limitation, a liquid fermentation method was developed, optimizing both fermentation conditions and medium components.

Serum Soluble Asialoglycoprotein Receptor 1: A Potential Predictor Marker Linked to Type 2 Diabetes Mellitus, Demonstrating Positive Correlation With High Sensitive C-Reactive Protein.

Background: Asialoglycoprotein receptor 1 (ASGR1) deficiency has been implicated in enhancing liver insulin sensitivity and reducing systemic insulin resistance, thus highlighting its pivotal role in glucose metabolism. However, the association between serum soluble asialoglycoprotein receptor 1 (sASGR1) and type 2 diabetes mellitus (T2DM) remains elusive. Therefore, we conducted a study to delve into this association.

Prediction of long-term stability of high-concentration formulations to support rapid development of antibodies against SARS-CoV-2.

Long-term stability of antibody therapeutics is required to ensure their safety and efficacy when administered to patients. However, obtaining shelf life supporting, long-term stability data are often a limiting factor for new drug candidates starting clinical trials. Predictive stability, which uses short-term accelerated stability data and kinetic modeling to forecast long-term storage stability, has the potential to provide justification to support establishing shelf life, although its application for biologics has only recently gained traction.

Advantages and limitations of 3,3',5,5'-tetramethylbenzidine for immunohistochemical staining.

In this study, two chromogenic systems, horseradish peroxidase (HRP)-3,3'-diaminobenzidine (DAB) and HRP-3,3',5,5'-tetramethylbenzidine (TMB), were used to perform single-color and double-color immunohistochemical staining (sIHC and dIHC, respectively) on multiple antigens in four distinct tissue types. The chromogenic results of the HRP-TMB system exhibited a vibrant blue-green color, and the tissue localization and signal intensity were consistent with those of the HRP-DAB system. In addition, it demonstrated clear differentiation from the hematoxylin-stained nucleus, endogenous melanin, and brown chromogenic results of HRP-DAB.

Mechanism of action of Danlou tablets affecting MAFLD via KEAP1-mediated oxeiptosis.

Ethnopharmacological Relevance: Metabolic fatty liver disease (MAFLD) is a significant risk factor for atherosclerotic cardiovascular disease. Several preliminary studies on MAFLD animal models have indicated the therapeutic potential of Danlou tablets (DLT), a primary Chinese medicine used for managing coronary artery disease. However, the underlying mechanism of DLT in the treatment of MAFLD remains elusive.

The landscape of cell regulatory and communication networks in the human dental follicle.

Introduction: The dental follicle localizes the surrounding enamel organ and dental papilla of the developing tooth germ during the embryonic stage. It can differentiate and develop to form the periodontal ligament, cementum, and alveolar bone tissues. Postnatally, the dental follicle gradually degenerates, but some parts of the dental follicle remain around the impacted tooth.

Comparative study of cannulated compression headless screws and anatomical locking compression hook plates for fracture of fifth metatarsal base in athletes and young adults.

Background: This study aimed to evaluate and compare the clinical effects and complications of cannulated compression headless screws (CHS) and anatomical locking compression hook plates (LCP) for fractures of the displaced fifth metatarsal base zone I in athletes and young adults.

Methods: Between May 2021 and June 2023, 50 patients were retrospectively evaluated. The patients were divided into two groups according to the fixation method: screw (CHS group, n = 20) or plate (LCP group, n = 30).

Control of Chirality and Directionality of Nonlinear Metasurface Light Source via Moiré Engineering.

Metasurfaces have revolutionized nonlinear and quantum light manipulation in the past decade, enabling the design of materials that can tune polarization, frequency, and direction of light simultaneously. However, tuning of metasurfaces is traditionally achieved by changing their microscopic structure, which does not allow in situ tuning and dynamic optimization of the metasurfaces. In this Letter, we explore the concept of twisted bilayer and tetralayer nonlinear metasurfaces, which offer rich tunability in its effective nonlinear susceptibilities.

The introduction of nano zero-valent iron in constructed wetlands simultaneously enhanced the removal of perfluorooctanoic acid (PFOA) and nutrients.

Constructed wetland (CW) serve as the final ecological barrier for hazardous materials entering the natural water environment. Due to the ecological toxicity and difficult bioutilization characteristics of perfluorooctanoic acid (PFOA) itself, CW technology faces great challenges in the field of PFOA remediation. In this study, nano zero-valent iron (nZVI) was introduced into CWs to explore the mechanism of the synergistic removal of PFOA and nutrients in nZVI-CW system.

Realization of Topology-Controlled Photonic Cavities in a Valley Photonic Crystal.

We report the experimental realization of a new type of topology-controlled photonic cavities in valley photonic crystals by adopting judiciously oriented mirrors to localize the valley-polarized edge states along their propagation path. By using microwave frequency- and time-domain measurements, we directly observe the strong confinement of electromagnetic energy at the mirror surface due to the extended time delay required for the valley index flipping. Moreover, we experimentally demonstrate that both the degree of energy localization and quality factors of the topology-controlled photonic cavities are determined by the valley-flipping time which is controlled by the topology of the mirror.

Single-cell nanocapsules of gut microbiota facilitate fecal microbiota transplantation.

Fecal microbiota transplantation (FMT) is advantageous for treating intractable diseases via the microbiota-gut-organ axis. However, invasive administration of gut microbiota via nasal feeding tubes limits the widespread application of FMT. Here, we attempted to develop a novel strategy to deliver gut microbiota using nanocapsules.

Lipidomics-based investigation of its impact on the pathogenesis of coronary atherosclerosis: a Mendelian randomization study.

Background: Considerable attention has been devoted to investigating the association between lipid metabolites and cardiovascular diseases, particularly coronary atherosclerosis.

Methods: A two-sample MR framework was used to investigate the relationship between lipid metabolites and the risk of coronary atherosclerosis. Two GWAS datasets were examined to take intersections of SNPs from 51,589 cases and 343,079 controls, and 14,334 cases and 346,860 controls to determine genetic susceptibility to coronary atherosclerosis.

Stereoselective Reaction Enabling Simultaneous Analysis of Carbon-Carbon Double-Bond Configuration and the Position of Monounsaturated Fatty Acids through UHPLC-ESI-MRM-MS.

Monounsaturated fatty acids (MUFA) are an important class of nutrients and are involved in lipid metabolism. The positions of the C=C bond and cis-trans isomerism have a significant influence on their physiological activity. However, simultaneously detecting these two structural properties has been challenging due to multiple isomers of MUFA.

Decreases in frequency-dependent intrinsic activity of the default mode network are associated with depression and cognition in patients with postacute sequelae of SARS-CoV-2 infection.

A significant proportion of patients who have recovered from COVID-19 suffer from persistent symptoms, referred to as "post-acute sequelae of SARS-CoV-2 infection (PASC)". Abnormal brain intrinsic activity has been observed in PASC patients, but the patterns of frequency-dependent intrinsic activity in the PASC and non-PASC (recovered COVID-19 patients without persistent symptoms) groups and their association with neuropsychiatric sequelae remain unclear in PASC. Twenty-nine PASC patients, 27 non-PASC subjects, and 31 healthy controls (HCs) were recruited.

Intolerance of uncertainty, aging, and anxiety and mental health concerns: A scoping review and meta-analysis.

Although intolerance of uncertainty (IU) is associated with negative outcomes, studies focusing on older adults are still emerging. Specifically, the relationship between IU and psychological health in this population remains unclear. Moreover, no review has focused on understanding the unique contributions of IU and aging to anxiety and mental health in older adults.

Administration of non-national immunization program vaccines for children under six in a rural county, Henan Province: Did costs matter?

This study aimed to investigate caregivers' administration of non-National Immunization Program (NIP) vaccines in rural China, and examine health system, individual, and social determinants. A cross-sectional survey ( = 1051) was conducted from July to October in 2022 in a rural county of Henan Province. Caregivers of children under six who came to township health centers for child vaccination were interviewed.

Mitoxantrone inhibits and downregulates ER through binding at the DBD-LBD interface.

Targeting the estrogen receptor (ER or ERα) through competitive antagonists, receptor downregulators, or estrogen synthesis inhibition remains the primary therapeutic strategy for luminal breast cancer. We have identified a novel mechanism of ER inhibition by targeting the critical interface between its DNA-binding domain (DBD) and ligand-binding domain (LBD). We demonstrate that mitoxantrone (MTO), a topoisomerase II inhibitor, binds at this previously unexplored DBD-LBD interface.

Optimizing natural human-derived decellularized tissue materials for periodontal bone defect repair.

Periodontal disease is a major contributor to tooth loss worldwide in adults. Particularly, periodontal bone defect is a common clinical condition, yet current therapeutic strategies exhibit limited effectiveness. Recently, natural bone graft materials have attracted considerable interest for enhancing bone defect repair due to their superior biocompatibility and osteogenic capabilities.

Extracellular AMP Inhibits Pollen Tube Growth in via Disrupted Calcium Gradient and Disorganized Microfilaments.

Adenosine monophosphate (AMP) is a hydrolysis product of adenosine triphosphate (ATP) and adenosine diphosphate (ADP). In mammalian cells, extracellular AMP functions as a signaling molecule by binding to adenosine A1 receptors, thereby activating various intracellular signaling pathways. However, the role of extracellular AMP in plant cells remains largely unclear, and homologs of A1 receptors have not been identified.

Spin Glass State and Griffiths Phase in van der Waals Ferromagnetic Material FeGeTe.

The discovery of two-dimensional (2D) van der Waals ferromagnetic materials opens up new avenues for making devices with high information storage density, ultra-fast response, high integration, and low power consumption. FeGeTe has attracted much attention because of its ferromagnetic transition temperature near room temperature. However, the investigation of its phase transition is rare until now.

GP73 reinforces cytotoxic T-cell function by regulating HIF-1α and increasing antitumor efficacy.

Background: Immunotherapy that targets immune checkpoints has achieved revolutionary success, but its application in solid tumors remains limited, highlighting the need for reliable enhancement of the efficacy of immunotherapy. Golgi protein 73 (GP73), a Golgi membrane protein, has been implicated in various cellular processes, including immune regulation. Recent studies suggested that GP73 may play a role in modulating the immune response in patients with cancer.