Identification of a Novel Activated NK-Associated Gene Score Associated with Diagnosis and Biological Therapy Response in Ulcerative Colitis.

Introduction: Natural killer (NK) cells are associated with the pathogenesis of ulcerative colitis (UC); however, their precise contributions remain unclear. The present study aimed to investigate the diagnostic value of the activated NK-associated gene (ANAG) score in UC and evaluate its predictive value in response to biological therapy.

Methods: Bulk RNA-seq and scRNA-seq datasets were obtained from the Gene Expression Omnibus (GEO) and Single Cell Portal (SCP) databases.

Constraints on Ultraheavy Dark Matter Properties from Dwarf Spheroidal Galaxies with LHAASO Observations.

In this Letter we try to search for signals generated by ultraheavy dark matter at the Large High Altitude Air Shower Observatory (LHAASO) data. We look for possible γ rays by dark matter annihilation or decay from 16 dwarf spheroidal galaxies in the field of view of the LHAASO. Dwarf spheroidal galaxies are among the most promising targets for indirect detection of dark matter that have low fluxes of astrophysical γ-ray background while having large amount of dark matter.

Variants in NLRP2 and ZFP36L2, non-core components of the human subcortical maternal complex, cause female infertility with embryonic development arrest.

The subcortical maternal complex (SCMC), which is vital in oocyte maturation and embryogenesis, consists of core proteins (NLRP5, TLE6, OOEP), non-core proteins (PADI6, KHDC3L, NLRP2, NLRP7), and other unknown proteins that are encoded by maternal effect genes. Some variants of SCMC genes have been linked to female infertility characterized by embryonic development arrest. However, so far, the candidate non-core SCMC components associated with embryonic development need further exploration and the pathogenic variants that have been identified are still limited.

The contribution of dietary advanced glycation end-products and genetic risk in the development of inflammatory bowel disease: a prospective cohort study.

Background: Dietary advanced glycation end products (AGEs) may promote oxidative stress and inflammation in the gastrointestinal tract.

Aims: The aim of this study is to investigate the association between dietary AGE intake and the risk of inflammatory bowel disease (IBD).

Methods: We included 121,978 participants without IBD at baseline from the UK Biobank.

Thin Film of Poly-peri-naphthalene (PPN) Fabricated by Direct Laser Writing and Its Use for Humidity Sensing.

Inspired by the versatility of the direct laser writing carbonization (DLWc) technique as well as the metal-ion-assisted coordination polymer of 3,4,9,10-perylene tetracarboxylic dianhydride (PTCDA), in the present study, we present a DLWc-enabled approach for cost-effectively fabricating a poly-peri-naphthalene (PPN) thin film with the advantages of patternability, environmental benignity, and scalability. Optical and scanning electron microscopy, as well as ultraviolet-visible-near-infrared, Fourier transform infrared, Raman, and X-ray photoelectron spectroscopies, were performed to confirm that the spray-coated thin film of the Mg-PTCDA coordination polymer can be in situ converted into a PPN thin film upon CO laser irradiation. The effects of the laser power and Mg concentration on the structure and electrical properties of the laser-processed PPN thin films were investigated.

Regulating intermolecular hydrogen bonding to construct solvation-induced emission shift quinoline derivatives for real-time monitoring of water content in organic solvents.

Water constitutes the most prevalent impurity in organic solvents, exerting significant influence on chemical reactions and potentially leading to fires and explosions, even in minute quantities. Thus, the development of convenient, rapid, and cost effective fluorescent probes for real-time monitoring of water content in organic solvents is imperative. Although some fluorescent materials have been synthesized for this purpose, most suffer from laborious preparation processes and poor cycling performance, constraining their practical application.

SOX2 interacts with hnRNPK to modulate alternative splicing in mouse embryonic stem cells.

Background: SOX2 is a determinant transcription factor that governs the balance between stemness and differentiation by influencing transcription and splicing programs. The role of SOX2 is intricately shaped by its interactions with specific partners. In the interactome of SOX2 in mouse embryonic stem cells (mESCs), there is a cohort of heterogeneous nuclear ribonucleoproteins (hnRNPs) that contributes to multiple facets of gene expression regulation.

Bioequivalence study of two formulations of afatinib dimaleate tablets in healthy subjects under fasting conditions: A randomized, open-label, single-dose, crossover trial.

Objective: To evaluate the bioequivalence of two different afatinib dimaleate formulations in healthy Chinese subjects under fasting conditions and to assess their pharmacokinetic and safety profiles.

Materials And Methods: This randomized, open-label, 2-period, crossover, bioequivalence study included 32 healthy Chinese subjects. The subjects were assigned to receive a single 40-mg dose of generic or brand-named afatinib dimaleate tablet.

Development and validation of a rapid five-minute nucleic acid extraction method for respiratory viruses.

Background: The rapid transmission and high pathogenicity of respiratory viruses significantly impact the health of both children and adults. Extracting and detecting their nucleic acid is crucial for disease prevention and treatment strategies. However, current extraction methods are laborious and time-consuming and show significant variations in nucleic acid content and purity among different kits, affecting detection sensitivity and efficiency.

A magnetic resonance imaging-based lymph node regression grading scheme for nasopharyngeal carcinoma after radiotherapy.

Background: Among patients with nasopharyngeal carcinoma (NPC), there is no established method to distinguish between patients with residual disease that may eventually progress and those who have achieved cured. We thus aimed to assess the prognostic value of magnetic resonance imaging (MRI)-based lymph node regression grade (LRG) in the risk stratification of patients with NPC following radiotherapy (RT).

Methods: This study retrospectively enrolled 387 patients newly diagnosed with NPC between January 2010 and January 2013.

In-situ electrospinning PVB/Camellia oil/ZnO-TiO nanofibrous membranes with synergistic antibacterial and degradation of ethylene applied in fruit preservation.

This work utilizes a handheld electrospinning device to prepare a novel nanofibrous composite membrane in situ for packaging freshness. It can realize pick-and-pack and is easy to operate. The nanofibrous membrane is based on PVB as the matrix material, adding Camellia oil (CO) and ZnO-TiO composite nanoparticles (ZT) as the active material.

Prospective study of Tc-3PRGD SPECT/CT diagnosing metastatic lymph nodes in esophageal squamous cell carcinoma.

Background: Lymph node (LN) metastasis is a significant prognostic factor for esophageal squamous cell carcinoma (ESCC), and there are no satisfactory methods for accurately predicting metastatic LNs. The present study aimed to assess the efficacy of Tc-3PRGD single-photon emission computed tomography (SPECT)/computed tomography (CT) for diagnosing metastatic LNs in ESCC.

Methods: A total of 15 enrolled patients with ESCC underwent Tc-3PRGD SPECT/CT and 18F-fluorodeoxyglucose positron emission tomography-computed tomography (F-FDG PET/CT) examinations preoperatively.

Developing a Machine-Learning Prediction Model for Infliximab Response in Crohn's Disease: Integrating Clinical Characteristics and Longitudinal Laboratory Trends.

Background: Achieving long-term clinical remission in Crohn's disease (CD) with antitumor necrosis factor α (anti-TNF-α) agents remains challenging.

Aims: This study aims to establish a prediction model based on patients' clinical characteristics using a machine-learning approach to predict the long-term efficacy of infliximab (IFX).

Methods: Three cohorts comprising 746 patients with CD were included from 3 inflammatory bowel disease (IBD) centers between June 2013 and January 2022.

Hydroxyl radical mediated extracellular degradation of tetracycline under aerobic and anaerobic conditions stimulated by bio-FeS nanoparticles.

The extracellular degradation of antibiotics facilitated by bio-nanoparticles is significant in the field of waste valorization. Among different bio-nanoparticles, bio-FeS nanoparticles stand out for their convenient and cost-effective synthesis. Nevertheless, there is a lack of understanding regarding the extracellular degradation of pollutants driven by bio-FeS nanoparticles.

A Mutation Losing an RBP-Binding Site in the LncRNA NORSF Transcript Influences Granulosa Cell Apoptosis and Sow Fertility.

Sow fertility is an economically important quantitative trait. Hundreds of quantitative trait loci (QTLs) containing tens of thousands of potential candidate genes are excavated. However, among these genes, non-coding RNAs including long non-coding RNAs (lncRNAs) are often overlooked.

Flow tweezing of anisotropic magnetic microrobots in a dynamic magnetic trap for active retention and localized flow sensing.

Controlled manipulation of microscale robotic devices in complex fluidic networks is critical for various applications in biomedical endovascular sensing, lab-on-chip biochemical assays, and environmental monitoring. However, achieving controlled transport and active retention of microscale robots with flow sensing capability has proven to be challenging. Here, we report the dynamic tweezing of an anisotropic magnetic microrobot in a rotating magnetic trap for active retention and localized flow sensing under confined fluidic conditions.

Synthesis of shape-controlled covalent organic frameworks for light scattering detection of iron and chromium ions.

Fabricating covalent organic frameworks with different morphologies based on the same structural motifs is both interesting and challenging. Here, a TTA-TFP-COF was synthesized by both solvothermal and room temperature methods, with 2,4,6-Tris(4-aminophenyl)-1,3,5-triazine (TTA) and 1,3,5-tris(4-formylphenyl)-benzene (TFP) as raw material. Using different synthesis conditions and adding aniline and benzaldehyde as regulators in the synthesis process, we found that these processes could slow down the reaction speed, increase the exchange and metathesis reactions of dynamic reversible reactions, and improve the reversibility of the reaction system.

TNF-α and RPLP0 drive the apoptosis of endothelial cells and increase susceptibility to high-altitude pulmonary edema.

High-altitude pulmonary edema (HAPE) is a fatal threat for sojourners who ascend rapidly without sufficient acclimatization. Acclimatized sojourners and adapted natives are both insensitive to HAPE but have different physiological traits and molecular bases. In this study, based on GSE52209, the gene expression profiles of HAPE patients were compared with those of acclimatized sojourners and adapted natives, with the common and divergent differentially expressed genes (DEGs) and their hub genes identified, respectively.

An immunosensor for the near real-time and site of inflammation detections of multiple proinflammatory cytokines.

Diseases mediated by cytokine storms are often characterized by an overexuberant pace of pathogenesis accompanied by significant morbidity and mortality. Thus, near real-time (NRT) detections via a site-of-inflammation (SOI) sampling of proinflammatory cytokines are essential to ensure a timely and effective treatment of acute inflammations, which up to now, has not been fully possible. In this work, we proposed a novel NRT and SOI immunosensor using ZIF-8 signal amplification together with an off-on strategy.

Gasdermin-E-mediated pyroptosis drives immune checkpoint inhibitor-associated myocarditis via cGAS-STING activation.

Immune checkpoint inhibitor (ICI)-induced myocarditis involves intensive immune/inflammation activation; however, its molecular basis is unclear. Here, we show that gasdermin-E (GSDME), a gasdermin family member, drives ICI-induced myocarditis. Pyroptosis mediated by GSDME, but not the canonical GSDMD, is activated in myocardial tissue of mice and cancer patients with ICI-induced myocarditis.

pharmacokinetics of polysaccharides.

polysaccharides (GUPS) are widely applied in biomedicine and functional food due to their multiple pharmacological activities and low toxicity. Despite their widespread use, the metabolic profile of GUPS remains poorly understood. To address this gap, we developed a quantitative analysis method that involves labeling GUPS with visible fluorescein (5-DTAF) and near-infrared (NIR) fluorescein (Cy7), resulting in stable conjugates with substitution degrees of 0.