The impact of aztreonam-clavulanic acid exposure on gene expression and mutant selection using a multidrug-resistant .

Multidrug-resistant poses a significant threat to the healthcare system by causing treatment failure in infected patients. The use of a beta-lactam in combination with a beta-lactamase inhibitor has been shown to be an effective strategy to solve this problem. antimicrobial susceptibility experiments have demonstrated the antimicrobial activity of aztreonam and clavulanate.

Multi-omics reveals immune response and metabolic profiles during high-altitude mountaineering.

The physiological perturbations induced by high-altitude exposure in mountain climbers, manifesting as immunological and metabolic deviations, have been previously reported but are not fully understood. In this study, we obtain multi-omic profiles of climbers' blood samples, including single-cell transcriptomic analysis of 375,722 immune cells, and plasma metabolomics and lipidomics. Longitudinal analysis reveals dynamic immune response profiles, during the acclimatization period, characterized by the downregulation of inflammatory responses in myeloid cell subsets and by the enhancement of immune effector processes in cytotoxic CD8 T, γδT, and CD16 natural killer cells.

Synthesis, biological and pharmacokinetic characterization of a novel leucine ureido derivative as a multi-target anticancer agent.

Previously, a novel series of leucine ureido derivatives containing the 1,2,3-triazole moiety were identified and validated as potent aminopeptidase N inhibitors with marked and antitumor potencies. Moreover, synergistic anti-proliferation effects against tumor cells were found when used in combination with 5-Fluorouracil (5-FU). Herein, a novel leucine ureido derivative (compound 3) was synthesized by coupling cytotoxic agent 5-FU with leucine ureido derivatives containing the 1,2,3-triazole moiety esterification.

Nucleoside reverse transcriptase inhibition enhances platelet production and megakaryocyte maturation in patients with COVID-19.

Coronavirus disease 2019 (COVID-19) is a systemic infection frequently involving the haematopoietic system. Thrombocytopenia is associated with increased risks of severe disease progression and mortality. Antiviral agents have shown much promise in decreasing viral load and shortening the time to the resolution of symptoms.

SMYD4 promotes MYH9 ubiquitination through lysine monomethylation modification to inhibit breast cancer progression.

Background: Breast cancer is the leading cause of female mortality worldwide. (SET And MYND Domain Containing 4) SMYD4 has been reported to be a tumour suppressor. However, the molecular mechanism of SMYD4 remains unclear.

SEC62-dependent ER-phagy contributes to apelin-13/APJ-induced monocyte-vascular endothelial cell adhesion in atherosclerosis pathogenesis.

The monocyte adhesion to vascular endothelial cells constitutes a key step in atherosclerosis pathogenesis. We previously found that ROS-autophagy pathway participated in the monocyte-endothelial cell adhesion induced by angiotensin domain type 1 receptor-associated proteins (APJ) and its endogenous ligand apelin-13. In this study, we investigated what specific type of autophagy apelin-13 regulated in this process.

Predictive value and mediating effect analysis of the AHR-ARNT-CYP1A1 axis for missed abortion related to polycyclic aromatic hydrocarbons exposure.

This study aimed to determine the predictive value of aryl hydrocarbon receptor (AHR) signaling pathway genes, and to detect the mediating effect of AHR that may mediate the influence of polycyclic aromatic hydrocarbons (PAHs) on missed abortion. The present study was conducted using a 1:2 matched case-control study, a total of 94 cases of women with missed abortion and 188 matched women controls with normal pregnancy but voluntarily abortion were included. The receiver operating characteristic curve (ROC) and the area under the ROC curve (AUC) were used to evaluate the predictive value of AHR signaling pathway genes for missed abortion.

Green Polymer Derived Multifunctional Layer Achieving Oriented Diffusion and Controllable Deposition of Zn for Ultra-Durable Zinc-Ion Hybrid Supercapacitors.

Rampant dendrite growth and severe parasitic reactions at the electrode/electrolyte interface significantly limit the cycle life of aqueous zinc ion hybrid supercapacitors (ZHSCs). In this study, sodium lignosulfonate (SLS) as one green polymer was introduced into ZnSO electrolyte to construct a multifunctional layer on the surface of Zn plates. Experimental analyses and theoretical calculations show that the presence of the SLS layer, rich in oxygen-containing functional groups (-SO), can not only modulate the structure of the electric double layer (EDL) to suppress interfacial side reactions caused by free HO and SO, but also promote (101)-oriented deposition by selectively controlling the deposition behavior of Zn through specific adsorption on different crystalline surfaces.

Ecological stoichiometry in leaves, branches, and soils of at different stand ages in the Taihang Mountains, China.

To understand the changes and relationship of plant-soil eco-stoichiometric characteristics of plantations with stand ages, we investigated plantations of different stand ages (10, 23, 39, and 47 years old) in the Taihang Mountains. We measured the concentration and stoichiometry of carbon (C), nitrogen (N), and phosphorus (P) in leaves, branches, and soils at different layers. We analyzed the relationship between leaf, branch nutrient characteristics and soil physicochemical properties.

Association between Fish Consumption and Stroke Incidence Across Different Predicted Risk Populations: A Prospective Cohort Study from China.

Objective: The relationship between fish consumption and stroke is inconsistent, and it is uncertain whether this association varies across predicted stroke risks.

Methods: A cohort study comprising 95,800 participants from the Prediction for Atherosclerotic Cardiovascular Disease Risk in China project was conducted. A standardized questionnaire was used to collect data on fish consumption.

LbCas12a-based DNA POCT facilitates fast genotyping on farm.

Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 12a (CRISPR/Cas12a) detection system is now widely used for nucleic acid detection and disease diagnosis. However, there are still fewer detections for single nucleotide polymorphisms (SNPs) and limited diversified detection systems for pathogen and SNP sites detection, which greatly limits their applications. Obviously, the development of a more diversified and convenient suite of detection tools is essential to unlock the full potential of CRISPR/Cas12a technology and to expand its applications across a wider range of scenarios.

Factors affecting compliance with oral anticoagulant medication for secondary prevention in middle-aged and young patients with coronary heart disease after percutaneous coronary intervention and the establishment of a prediction model.

Patients with coronary heart disease (CHD) who undergo percutaneous coronary intervention (PCI) may exhibit suboptimal adherence to oral anticoagulant therapy due to various factors. This study aims to investigate the determinants influencing adherence to oral anticoagulant therapy in middle-aged and young CHD patients following PCI. We conducted a retrospective analysis on the clinical data of 310 middle-aged and young CHD patients who underwent PCI treatment at The Fourth Affiliated Hospital of Zhejiang University School of Medicine between 6 month 2023 and 8 month 2024, monitored for 6 months and categorized them into a non-compliance group (n=86) and a compliance group (n=224).

Relationship of monocyte to high-density lipoprotein ratio (MHR) and other inflammatory biomarkers with sarcopenia: a population-based study.

Objectives: In previous studies, several inflammatory biomarkers derived from complete blood cell counts (CBC), such as systemic immune inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), and non‑high‑density lipoprotein cholesterol to high‑density lipoprotein cholesterol ratio (NHHR) have been identified as predictors of sarcopenia. However, whether Monocyte to High-Density Lipoprotein Cholesterol Ratio (MHR) can predict the development of sarcopenia has not yet been established. The research first attempts to investigate the association between MHR and low muscle mass and to compare the predictive abilities of MHR, SII, NLR, and NHHR for low muscle mass risk.

Mechanistic insights into PROS1 inhibition of bladder cancer progression and angiogenesis via the AKT/GSK3β/β-catenin pathway.

Bladder cancer (BLCA) is one of the ten most common cancers worldwide. However, the deregulation of PROS1 and its specific function in BLCA is not well understood. By combining proteomic and transcriptomic datasets, we discovered PROS1 expression was significantly reduced in BLCA tissues and revealed the clinical relevance of PROS1 with BLCA.

Transcriptome and chromatin accessibility landscape of ovarian development at different egg-laying stages in taihe black-bone silky fowls.

Taihe Black-Bone Silky Fowl (SF) is a famous local breed in China, known for its high nutritional and medicinal value. However, its low egg-laying rate significantly limits its economic benefits. This study aims to explore the ovarian development status, as well as the changes in the transcriptome and chromatin accessibility landscape at different egg-laying stages of SF, in order to reveal the epigenetic regulatory mechanisms underlying ovarian development in laying hens.

Differences in starch and protein composition, morphological and structure, and their impacts on eating quality of soft japonica rice under different light and nitrogen fertilizer conditions in southern China.

This study explores the differences in starch and protein composition, morphological and structure, and their impacts on soft japonica rice eating quality under different light and nitrogen fertilizer conditions. Results showed that decreased light and applied panicle fertilize resulted in a decreased in total starch, accompanied by an increased in long-chain amylopectin, protein, particularly glutelin, and β-sheet, with these effects being more pronounced when panicle fertilizer was applied under 50 % light. RVA, LF-NMR and Rheometer date showed that aforementioned changes in starch and protein were detrimental to water migration and starch gelatinization during rice cooking, resulting in a high-strength rice gel network.

Nudix Hydrolase 13 Impairs the Initiation of Colorectal Cancer by Inhibiting PKM1 ADP-Ribosylation.

Metabolic dysregulation has been implicated as a key factor in colorectal cancer (CRC) initiation, however, the underlying driving forces and mechanisms remain poorly understood. Herein, transcriptome profiling of paired early-stage CRCs and adenomas identifies Nudix hydrolase 13 (NUDT13) as a critical suppressor. Elevated NUDT13 expression impedes the proliferation of CRC cells under hypoxic conditions and markedly inhibits CRC initiation by upregulating PKM1.

Leveraging genomic insights from the neglected malaria parasites P. malariae and P. ovale using selective whole genome amplification (SWGA) approach.

Background: Systematic genomics-guided population-based studies on the neglected malaria parasites, P. malariae, P. ovale curtisi, and P.

Anisotropic Quasi-particle Decay Dynamics in Antiferromagnetic CuCrPS Single Crystals.

Two-dimensional van der Waals material CuCrPS (CCPS) has attracted significant attention for its coexistence of antiferromagnetic and antiferroelectric states, which have been reported to give rise to strong optical anisotropy. Here, we investigate its anisotropic quasi-particle decay dynamics using polarization-resolved femtosecond transient optical spectroscopy. We observe that the quasi-particle decay dynamics in the CCPS single crystal strongly correlate with its spin ordering.

Clinical biomarkers of perioperative neurocognitive disorder: initiation and recommendation.

Perioperative neurocognitive disorder (PND) is a significant neurological complication in aging perioperative patients that impacts post-operative cognition. PND is currently diagnosed through cognitive function testing, which is limited by its subjectivity and time requirements. Thus, the identification of biomarkers to assess PND onset is a priority to identify at-risk individuals and enable interventions and treatments to patient outcomes.

Study on immortalization of Mongolian sheep fibroblast cells.

This study aims to establish an immortalized fibroblast cell line from Mongolian sheep. Primary Mongolian sheep fibroblasts (SSF) were isolated using tissue explant and enzymatic digestion methods, followed by microscopic observation, growth curve plotting, and karyotype analysis. The results confirmed the successful isolation of SSF.

Overcoming immunotherapy resistance in hepatocellular carcinoma by targeting myeloid IL-8/CXCR2 signaling.

Durable responses to immune checkpoint blockade (ICB) in hepatocellular carcinoma (HCC) are limited to a minority of patients, yet reliable biomarkers are still lacking. Inflammatory cytokines such as interleukin-8 (IL-8) are associated with HCC progression, and IL-8 is known as the chemoattractant for immunosuppressive myeloid cells. Therefore, we aim to elucidate the ICB resistance mechanisms mediated by the activation of the IL-8/CXCR2 pathway.

Three-dimensional cellulose acetate nanofiber aerogels: A novel platform for ratiometric fluorescence sensing of volatile amines in aquatic products.

This study presents the development of cellulose acetate nanofiber aerogels (CA NFAs) as a novel solid-phase carrier for ratiometric fluorescence sensors, designed for detecting volatile amines, which is critical for monitoring the freshness of aquatic products. The sensors were functionalized with fluorescein isothiocyanate (FITC) as an indicator and protoporphyrin IX (PPIX) as an internal reference, which were covalently attached to the cellulose backbone. The FITC/PPIX-CA NFAs were fabricated using electrospinning and freeze-drying techniques, resulting in a 3D porous structure that significantly enhanced the mass transfer of target gas molecules and their interaction with probe molecules.

Anti-tumor effects of artemisinin-based oligomers: From monomer to trimer as a novel drug-enhancing strategy.

Artemisinin and its derivatives (ARTs) are being studied for their potential anti-tumor activity. Dimerization of artemisinin has been proposed as a promising means of enhancing drug efficacy. However, the sequential progression from monomers to dimers and trimers, retaining a consistent β-configuration, has not been previously investigated in terms of its effect on compound activity.