Publications by authors named "Cansu Pinar Yenice"
Article Synopsis
- The detection of single nucleotide polymorphisms (SNPs) is crucial for clinical diagnostics, pharmacogenomics, and forensics, particularly for identifying genetic risks like those associated with osteoporosis.
- A semiautomated system using solid-phase electrochemical melting curve analysis (éMCA) was developed to identify alleles at specific SNP sites related to bone fracture risks by employing asymmetric isothermal recombinase polymerase amplification.
- The proof-of-concept utilized a microfluidic device with a multielectrode array, allowing for the effective detection of SNP hetero/homozygosity, particularly at the osteoporosis-related SNP site rs2741856 in real patient samples.
The previously reported approach of orthogonal multipotential redox coding of all four DNA bases allowed only analysis of the relative nucleotide composition of short DNA stretches. Here, we present two methods for normalization of the electrochemical readout to facilitate the determination of the total nucleotide composition. The first method is based on the presence or absence of an internal standard of 7-deaza-2'-deoxyguanosine in a DNA primer.
View Article and Find Full Text PDFWe report a series of 2'-deoxyribonucleoside triphosphates bearing dicarba--undecaborate ([CBH]), [3,3'-iron-bis(1,2-dicarbollide)] (FESAN, [Fe(CBH)]) or [3,3'-cobalt-bis(1,2-dicarbollide)] (COSAN, [Co(CBH)]) groups prepared either through the Sonogashira cross-coupling or the CuAAC click reaction. The modified s were substrates for KOD XL DNA polymerase in enzymatic synthesis of modified DNA through primer extension (PEX). The -carborane- and FESAN-modified nucleotides gave analytically useful oxidation signals in square-wave voltammetry and were used for redox labeling of DNA.
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