Giardia intestinalis: expression of ubiquitin, glucosamine-6-phosphate and cyst wall protein genes during the encystment process.

Unlabelled: We determined the relative expression of ubiquitin (ub), glucosamine-6-phosphate-isomerase (gn6pi) and cyst wall protein (cwp) genes during encystment of the Portland-1 and Portland-1R strains of Giardia intestinalis. Encystment was induced with bile for different time periods. The presence of encystment-specific vesicles (ESVs) and the relative expression of genes (log(10)ΔRn) were determined by transmission electron microscopy and real-time PCR, respectively.